Now showing items 1-4 of 4

    • High-Resolution Analysis of Parent-of-Origin Allelic Expression in the Mouse Brain 

      Gregg, Christopher; Zhang, Jiangwen; Weissbourd, Brandon; Luo, Shujun; Schroth, Gary P.; Haig, David Addison; Dulac, Catherine (American Association for the Advancement of Science (AAAS), 2010)
      Genomic imprinting results in preferential expression of the paternal or maternal allele of certain genes. We have performed a genome-wide characterization of imprinting in the mouse embryonic and adult brain. This approach ...
    • Improving Lambda Red Genome Engineering in Escherichia coli via Rational Removal of Endogenous Nucleases 

      Mosberg, Joshua Adam Weintrob; Gregg, Christopher; Lajoie, Marc Joseph; Wang, Harris He; Church, George McDonald (Public Library of Science, 2012)
      Lambda Red recombineering is a powerful technique for making targeted genetic changes in bacteria. However, many applications are limited by the frequency of recombination. Previous studies have suggested that endogenous ...
    • Manipulating Replisome Dynamics to Enhance Lambda Red-Mediated Multiplex Genome Engineering 

      Lajoie, Marc Joseph; Gregg, Christopher; Mosberg, Joshua Adam Weintrob; Washington, G. C.; Church, George McDonald (Oxford University Press, 2012)
      Disrupting the interaction between primase and helicase in Escherichia coli increases Okazaki fragment (OF) length due to less frequent primer synthesis. We exploited this feature to increase the amount of ssDNA at the ...
    • Rational optimization of tolC as a powerful dual selectable marker for genome engineering 

      Gregg, Christopher J.; Lajoie, Marc J.; Napolitano, Michael G.; Mosberg, Joshua A.; Goodman, Daniel B.; Aach, John; Isaacs, Farren J.; Church, George M. (Oxford University Press, 2014)
      Selection has been invaluable for genetic manipulation, although counter-selection has historically exhibited limited robustness and convenience. TolC, an outer membrane pore involved in transmembrane transport in E. coli, ...