Browsing by Author "Christodoulou, Danos"
Now showing items 1-3 of 3
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Methods for comprehensive transcriptome analysis using next-generation sequencing and application in hypertrophic cardiomyopathy
Christodoulou, Danos C. (2013-10-08)Characterization of the RNA transcriptome by next-generation sequencing can produce an unprecedented yield of information that provides novel biologic insights. I describe four approaches for sequencing different aspects ... -
Stable Gene Targeting in Human Cells Using Single-Strand Oligonucleotides with Modified Bases
Rios, Xavier; Briggs, Adrian; Christodoulou, Danos C.; Gorham, Josh McClean; Seidman, Jonathan G.; Church, George McDonald (Public Library of Science, 2012)Recent advances allow multiplexed genome engineering in E. coli, employing easily designed oligonucleotides to edit multiple loci simultaneously. A similar technology in human cells would greatly expedite functional genomics, ... -
Transcriptional Profiling of Cultured, Embryonic Epicardial Cells Identifies Novel Genes and Signaling Pathways Regulated by TGFβR3 In Vitro
DeLaughter, Daniel M.; Clark, Cynthia R.; Christodoulou, Danos C.; Seidman, Christine E.; Baldwin, H. Scott; Seidman, J. G.; Barnett, Joey V. (Public Library of Science, 2016)The epicardium plays an important role in coronary vessel formation and Tgfbr3-/- mice exhibit failed coronary vessel development associated with decreased epicardial cell invasion. Immortalized Tgfbr3-/- epicardial cells ...