Browsing by Author "Freudiger, Christian Wilhelm"

Now showing items 1-14 of 14

    • Coherent Nonlinear Optical Imaging: Beyond Fluorescence Microscopy 

      Min, Wei; Freudiger, Christian Wilhelm; Lu, Sijia; Xie, Xiaoliang Sunney (Annual Reviews, 2011)
      The quest for ultrahigh detection sensitivity with spectroscopic contrasts other than fluorescence has led to various novel approaches to optical microscopy of biological systems. Coherent nonlinear optical imaging, ...

    • Hyperspectral Imaging with Stimulated Raman Scattering by Chirped Femtosecond Lasers 

      Fu, Dan; Holtom, Gary; Freudiger, Christian Wilhelm; Zhang, Xu; Xie, Xiaoliang Sunney (American Chemical Society, 2013-03-22)
      Raman microscopy is a quantitative, label-free, and noninvasive optical imaging technique for studying inhomogeneous systems. However, the feebleness of Raman scattering significantly limits the use of Raman microscopy to ...

    • Intracavity Wavelength Modulation of an Optical Parametric Oscillator for Coherent Raman Microscopy 

      Saar, Brian Grady; Holtom, Gary; Freudiger, Christian Wilhelm; Ackermann, Chrisita; Hill, I. Winfield; Xie, Xiaoliang Sunney (Optical Society of America, 2009)
      We present a novel intracavity frequency modulation scheme in a tunable, picosecond optical parametric oscillator (OPO). The OPO signal wavelength can be modulated with a depth of more than 10 nm at a rate of 38 MHz (one ...

    • Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy 

      Freudiger, Christian Wilhelm; Min, Wei; Saar, Brian G.; Lu, Sijia; Holtom, Gary R.; He, Chengwei; Tsai, Jason C.; Kang, Jing Xuan; Xie, Xiaoliang Sunney (American Association for the Advancement of Science, 2008)
      Label-free chemical contrast is highly desirable in biomedical imaging. Spontaneous Raman microscopy provides specific vibrational signatures of chemical bonds, but is often hindered by low sensitivity. Here we report a ...

    • Label-Free Live-Cell Imaging of Nucleic Acids Using Stimulated Raman Scattering Microscopy 

      Zhang, Xu; Roeffaers, Maarten B. J.; Basu, Srinjan; Daniele, Joseph R.; Fu, Dan; Freudiger, Christian Wilhelm; Holtom, Gary R.; Xie, Xiaoliang Sunney (John Wiley and Sons, 2012)
      Imaging of nucleic acids is important for studying cellular processes such as cell division and apoptosis. A noninvasive label-free technique is attractive. Raman spectroscopy provides rich chemical information based on ...

    • Multicolor stimulated Raman scattering microscopy 

      Lu, Fake; Ji, Minbiao; Fu, Dan; Ni, Xiaohui; Freudiger, Christian Wilhelm; Holtom, Gary; Xie, Xiaoliang Sunney (Informa UK (Taylor & Francis), 2012)
      Stimulated Raman scattering (SRS) microscopy has opened up a wide range of biochemical imaging applications by probing a particular Raman-active molecule vibrational mode in the specimen. However, the original implementation ...

    • Multicolor Stimulated Raman Scattering Microscopy with a Rapidly Tunable Optical Parametric Oscillator 

      Kong, Lingjie; Ji, Minbiao; Holtom, Gary R.; Fu, Dan; Freudiger, Christian Wilhelm; Xie, Xiaoliang Sunney (Optical Society of America, 2013)
      Stimulated Raman scattering (SRS) microscopy allows label-free chemical imaging based on vibrational spectroscopy. Narrowband excitation with picosecond lasers creates the highest signal levels and enables imaging speeds ...

    • Multicolored Stain-Free Histopathology with Coherent Raman Imaging 

      Xie, Xiaoliang Sunney; Freudiger, Christian Wilhelm; Orringer, Daniel A.; Saar, Brian G.; Ji, Minbiao; Zeng, Qing; Ottoboni, Linda; Ying, Wei; Waeber, Christian; Sims, John R.; De Jager, Philip Lawrence; Sagher, Oren; Philbert, Martin A.; Xu, Xiaoyin; Kesari, Santosh; Young, Geoffrey; Pfannl, Rolf (Nature Publishing Group, 2012)
      Conventional histopathology with hematoxylin & eosin (H&E) has been the gold standard for histopathological diagnosis of a wide range of diseases. However, it is not performed in vivo and requires thin tissue sections ...

    • Quantitative Chemical Imaging with Multiplex Stimulated Raman Scattering Microscopy 

      Fu, Dan; Lu, Fake; Zhang, Xu; Freudiger, Christian Wilhelm; Pernik, Douglas R.; Holtom, Gary; Xie, Xiaoliang Sunney (American Chemical Society, 2012)
      Stimulated Raman scattering (SRS) microscopy is a newly developed label-free chemical imaging technique that overcomes the speed limitation of confocal Raman microscopy while avoiding the nonresonant background problem of ...

    • Raman Spectroscopy: Stimulated Raman Scattering Microscopy for Label-Free Chemical Imaging 

      Freudiger, Christian Wilhelm; Min, Wei; Saar, Brian; Xie, Xiaoliang Sunney (Optical Society of America, 2009)
      Coherent anti-Stokes Raman techniques are increasing the utility of Raman scattering for chemical and biological diagnostics.

    • Rapid, Label-Free Detection of Brain Tumors with Stimulated Raman Scattering Microscopy 

      Ji, Minbiao; Orringer, Daniel A.; Freudiger, Christian Wilhelm; Ramkissoon, Shakti; Liu, Xiaohui; Lau, Darryl; Golby, Alexandra Jacqueline; Norton, Isaiah Hakim; Hayashi, Marika; Agar, Nathalie Marie Yvonne Rachel; Young, Geoffrey; Spino, Cathie; Santagata, Sandro; Camelo-Piragua, Sandra; Ligon, Keith Lloyd; Sagher, Oren; Xie, Xiaoliang Sunney (American Association for the Advancement of Science (AAAS), 2013)
      Surgery is an essential component in the treatment of brain tumors. However, delineating tumor from normal brain remains a major challenge. We describe the use of stimulated Raman scattering (SRS) microscopy for ...

    • Stimulated Raman Scattering Microscopy with a Robust Fibre Laser Source 

      Freudiger, Christian; Yang, Wenlong; Holtom, Gary; Peyghambarian, Nasser; Xie, Xiaoliang Sunney; Kieu, Khanh Q. (Nature Publishing Group, 2014)
      Stimulated Raman scattering microscopy allows label-free chemical imaging and has enabled exciting applications in biology, material science and medicine. It provides a major advantage in imaging speed over spontaneous ...

    • Synchronized Time-Lens Source for Coherent Raman Scattering Microscopy 

      Wang, Ke; Freudiger, Christian Wilhelm; Lee, Jennifer H.; Saar, Brian G.; Xie, Xiaoliang Sunney; Xu, Chris (Optical Society of America, 2010)
      We use the time-lens concept to demonstrate a new scheme for synchronization of two pulsed light sources for biological imaging. An all fiber, 1064 nm time-lens source is synchronized to a picosecond solid-state Ti: Sapphire ...

    • Video Rate Molecular Imaging In Vivo with Stimulated Raman Scattering 

      Saar, Brian; Freudiger, Christian Wilhelm; Reichman, Jay; Stanley, C. Michael; Holtom, Gary; Xie, Xiaoliang Sunney (American Association for the Advancement of Science, 2010)
      Optical imaging in vivo with molecular specificity is important in biomedicine because of its high spatial resolution and sensitivity compared with magnetic resonance imaging. Stimulated Raman scattering (SRS) microscopy ...