Browsing by Author "Reinherz, Ellis"
Now showing items 1-20 of 23
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A computational method for identification of vaccine targets from protein regions of conserved human leukocyte antigen binding
Olsen, Lars R; Simon, Christian; Kudahl, Ulrich J; Bagger, Frederik O; Winther, Ole; Reinherz, Ellis L; Zhang, Guang L; Brusic, Vladimir (BioMed Central, 2015)Background: Computational methods for T cell-based vaccine target discovery focus on selection of highly conserved peptides identified across pathogen variants, followed by prediction of their binding of human leukocyte ... -
Conservation Analysis of Dengue Virus T-cell Epitope-Based Vaccine Candidates Using Peptide Block Entropy
Olsen, Lars Rønn; Zhang, Guang Lan; Keskin, Derin Benerci; Reinherz, Ellis Leonard; Brusic, Vladimir (Frontiers Research Foundation, 2011)Broad coverage of the pathogen population is particularly important when designing CD8+ T-cell epitope vaccines against viral pathogens. Traditional approaches are based on combinations of highly conserved T-cell epitopes. ... -
A Conserved Hydrophobic Patch on V\(\beta\) Domains Revealed by TCR\(\beta\) Chain Crystal Structures: Implications for Pre-TCR Dimerization
Zhou, Bo; Chen, Qiang; Mallis, Robert J.; Zhang, Hongmin; Liu, Jin-Huan; Reinherz, Ellis Leonard; Wang, Jiahuai (Frontiers Research Foundation, 2011)The \(\alpha\beta\) T cell receptor (TCR) is a multimeric complex whose \(\beta\) chain plays a crucial role in thymocyte development as well as antigen recognition by mature T lymphocytes. We report here crystal structures ... -
Direct Identification of an HPV-16 Tumor Antigen from Cervical Cancer Biopsy Specimens
Keskin, Derin Benerci; Reinhold, Bruce B.; Lee, Sun Young; Zhang, Guanglan; Lank, Simon; O’Connor, David H.; Berkowitz, Ross Stuart; Brusic, Vladimir; Kim, Seung Jo; Reinherz, Ellis Leonard (Frontiers Research Foundation, 2011)Persistent infection with high-risk human papilloma viruses (HPV) is the worldwide cause of many cancers, including cervical, anal, vulval, vaginal, penile, and oropharyngeal. Since T cells naturally eliminate the majority ... -
Elicitation from Virus-Naive Individuals of Cytotoxic T Lymphocytes Directed Against Conserved HIV-1 Epitopes
Reche, Pedro A.; Keskin, Derin Benerci; Hussey, Rebecca E.; Ancuta, Petronela; Gabuzda, Dana Helga; Reinherz, Ellis Leonard (BioMed Central, 2006)Cytotoxic T lymphocytes (CTL) protect against viruses including HIV-1. To avoid viral escape mutants that thwart immunity, we chose 25 CTL epitopes defined in the context of natural infection with functional and/or structural ... -
Elicitation of antibody responses against the HIV-1 gp41 Membrane Proximal External Region (MPER)
Cheng, Yuxing (2014-06-06)An effective vaccine to protect against HIV-1/AIDS remains elusive due to the extensive mechanisms employed by the HIV-1 virus to evade immune attack. Highly potent broadly neutralizing antibodies isolated from chronically ... -
Evaluation of MHC Class I Peptide Binding Prediction Servers: Applications for Vaccine Research
Lin, Hong Huang; Ray, Surajit; Tongchusak, Songsak; Reinherz, Ellis Leonard; Brusic, Vladimir (BioMed Central, 2008)Background: Protein antigens and their specific epitopes are formulation targets for epitope-based vaccines. A number of prediction servers are available for identification of peptides that bind major histocompatibility ... -
FluKB: A Knowledge-Based System for Influenza Vaccine Target Discovery and Analysis of the Immunological Properties of Influenza Viruses
Simon, Christian; Kudahl, Ulrich J.; Sun, Jing; Olsen, Lars Rønn; Zhang, Guang Lan; Reinherz, Ellis L.; Brusic, Vladimir (Hindawi Publishing Corporation, 2015)FluKB is a knowledge-based system focusing on data and analytical tools for influenza vaccine discovery. The main goal of FluKB is to provide access to curated influenza sequence and epitope data and enhance the analysis ... -
Forward Vaccinology: CTL Targeting Based upon Physical Detection of HLA-Bound Peptides
Reinherz, Ellis L.; Keskin, Derin B.; Reinhold, Bruce (Frontiers Media S.A., 2014)Vaccine-elicited cytotoxic T lymphocytes (CTL) recognizing conserved fragments of a pathogen’s proteome could greatly impact infectious diseases and cancers. Enabling this potential are recent advances in mass spectrometry ... -
HPVdb: a data mining system for knowledge discovery in human papillomavirus with applications in T cell immunology and vaccinology
Zhang, Guang Lan; Riemer, Angelika B.; Keskin, Derin B.; Chitkushev, Lou; Reinherz, Ellis L.; Brusic, Vladimir (Oxford University Press, 2014)High-risk human papillomaviruses (HPVs) are the causes of many cancers, including cervical, anal, vulvar, vaginal, penile and oropharyngeal. To facilitate diagnosis, prognosis and characterization of these cancers, it is ... -
Human Leukocyte Antigen Typing Using a Knowledge Base Coupled with a High-Throughput Oligonucleotide Probe Array Analysis
Zhang, Guang Lan; Keskin, Derin B.; Lin, Hsin-Nan; Lin, Hong Huang; DeLuca, David S.; Leppanen, Scott; Milford, Edgar L.; Reinherz, Ellis L.; Brusic, Vladimir (Frontiers Media S.A., 2014)Human leukocyte antigens (HLA) are important biomarkers because multiple diseases, drug toxicity, and vaccine responses reveal strong HLA associations. Current clinical HLA typing is an elimination process requiring serial ... -
Large-Scale Analysis of B-Cell Epitopes on Influenza Virus Hemagglutinin – Implications for Cross-Reactivity of Neutralizing Antibodies
Sun, Jing; Kudahl, Ulrich J.; Simon, Christian; Cao, Zhiwei; Reinherz, Ellis L.; Brusic, Vladimir (Frontiers Media S.A., 2014)Influenza viruses continue to cause substantial morbidity and mortality worldwide. Fast gene mutation on surface proteins of influenza virus result in increasing resistance to current vaccines and available antiviral drugs. ... -
Molecular T Cell Biology – Basic and Translational Challenges in the Twenty-First Century
Reinherz, Ellis Leonard; Acuto, Oreste (Frontiers Research Foundation, 2011) -
New Approaches to Eliciting Protective Immunity Through T Cell Repertoire Manipulation: The Concept of Thymic Vaccination
Fridkis-Hareli, Masha; Reinherz, Ellis Leonard (BioMed Central, 2004)Conventional vaccines afford protection against infectious diseases by expanding existing pathogen-specific peripheral lymphocytes, both CD8 cytotoxic effector (CTL) and CD4 helper T cells. The latter induce B cell maturation ... -
Plxnd1 Expression in Thymocytes Regulates Their Intrathymic Migration While That in Thymic Endothelium Impacts Medullary Topology
Choi, Young I.; Duke-Cohan, Jonathan S.; Tan, Jing; Gui, Jingang; Singh, Manvendra K.; Epstein, Jonathan A.; Reinherz, Ellis L. (Frontiers Media S.A., 2013)An important role for plexinD1 in thymic development is inferred from studies of germline Plxnd1 knockout (KO) mice where mislocalized CD69+ thymocytes as well as ectopic thymic subcapsular medullary structures were observed. ... -
PVS: A Web Server for Protein Sequence Variability Analysis Tuned to Facilitate Conserved Epitope Discovery
Garcia-Boronat, Maria; Diez-Rivero, Carmen M.; Reinherz, Ellis Leonard; Reche, Pedro A. (Oxford University Press, 2008)We have developed PVS (Protein Variability Server), a web-based tool that uses several variability metrics to compute the absolute site variability in multiple protein-sequence alignments (MSAs). The variability is then ... -
Pyrin Modulates the Intracellular Distribution of PSTPIP1
Waite, Andrea L.; Schaner, Philip; Richards, Neil; Balci-Peynircioglu, Banu; Masters, Seth L.; Brydges, Susannah D.; Hong, Arthur; Yilmaz, Engin; Kastner, Daniel L.; Gumucio, Deborah L.; May, Robin Charles; Fox, Michelle; Reinherz, Ellis Leonard (Public Library of Science, 2009)PSTPIP1 is a cytoskeleton-associated adaptor protein that links PEST-type phosphatases to their substrates. Mutations in PSTPIP1 cause PAPA syndrome (Pyogenic sterile Arthritis, Pyoderma gangrenosum, and Acne), an ... -
Revisiting the putative TCR Cα dimerization model through structural analysis
Wang, Jiahuai; Reinherz, Ellis Leonard (Frontiers Media S.A., 2013)Despite major advances in T cell receptor (TCR) biology and structure, how peptide–MHC complex (pMHC) ligands trigger αβ TCR activation remains unresolved. Two views exist. One model postulates that monomeric TCR–pMHC ... -
Significant Impact of Sequence Variations in the Nucleoprotein on CD8 T Cell-Mediated Cross-Protection against Influenza A Virus Infections
Zhong, Weimin; Liu, Feng; Dong, Libo; Lu, Xiuhua; Hancock, Kathy; Reinherz, Ellis Leonard; Katz, Jacqueline M.; Sambhara, Suryaprakash (Public Library of Science, 2010)Background: Memory CD8 T cells to influenza A viruses are widely detectable in healthy human subjects and broadly cross-reactive for serologically distinct influenza A virus subtypes. However, it is not clear to what extent ... -
Strict Major Histocompatibility Complex Molecule Class-Specific Binding by Co-Receptors Enforces MHC-Restricted αβ TCR Recognition during T Lineage Subset Commitment
Li, Xiao-Long; Teng, Mai-Kun; Reinherz, Ellis L.; Wang, Jia-Huai (Frontiers Media S.A., 2013)Since the discovery of co-receptor dependent αβTCR recognition, considerable effort has been spent on elucidating the basis of CD4 and CD8 lineage commitment in the thymus. The latter is responsible for generating mature ...