Now showing items 1-20 of 32

    • Adenosine signaling promotes hematopoietic stem and progenitor cell emergence 

      Jing, Lili; Tamplin, Owen J.; Chen, Michael J.; Deng, Qing; Patterson, Shenia; Kim, Peter G.; Durand, Ellen M.; McNeil, Ashley; Green, Julie M.; Matsuura, Shinobu; Ablain, Julien; Brandt, Margot K.; Schlaeger, Thorsten M.; Huttenlocher, Anna; Daley, George Q.; Ravid, Katya; Zon, Leonard I. (The Rockefeller University Press, 2015)
      Hematopoietic stem cells (HSCs) emerge from aortic endothelium via the endothelial-to-hematopoietic transition (EHT). The molecular mechanisms that initiate and regulate EHT remain poorly understood. Here, we show that ...
    • All-Trans Retinoic Acid Directs Urothelial Specification of Murine Embryonic Stem Cells via GATA4/6 Signaling Mechanisms 

      Mauney, Joshua Robert; Ramachandran, Aruna; Yu, Richard N.; Daley, George Quentin; Adam, Rosalyn Mare; Estrada, Carlos R. (Public Library of Science, 2010)
      The urinary bladder and associated tract are lined by the urothelium, a transitional epithelium that acts as a specialized permeability barrier that protects the underlying tissue from urine via expression of a highly ...
    • Biomechanical forces promote blood development through prostaglandin E2 and the cAMP–PKA signaling axis 

      Diaz, Miguel F.; Li, Nan; Lee, Hyun Jung; Adamo, Luigi; Evans, Siobahn M.; Willey, Hannah E.; Arora, Natasha; Torisawa, Yu-suke; Vickers, Dwayne A.; Morris, Samantha A.; Naveiras, Olaia; Murthy, Shashi K.; Ingber, Donald E.; Daley, George Q.; García-Cardeña, Guillermo; Wenzel, Pamela L. (The Rockefeller University Press, 2015)
      Blood flow promotes emergence of definitive hematopoietic stem cells (HSCs) in the developing embryo, yet the signals generated by hemodynamic forces that influence hematopoietic potential remain poorly defined. Here we ...
    • Broader Implications of Defining Standards for the Pluripotency of iPSCs 

      Daley, George Quentin; Lensch, Mathew William; Jaenisch, Rudolf; Meissner, Alexander; Plath, Kathrin; Yamanaka, Shinya (Elsevier BV, 2009)
      We read with great interest the excellent review by Maherali and Hochedlinger (2008) that recommends standards for characterization of pluripotent stem cell lines, especially the many new lines being generated using ...
    • Cdx4 Is Dispensable for Murine Adult Hematopoietic Stem Cells but Promotes MLL-AF9-Mediated Leukemogenesis 

      Koo, Sumin; Huntly, Brian J.; Wang, Yuan; Chen, Jing; Brumme, Kristina; Ball, Brian; McKinney-Freeman, Shannon L.; Yabuuchi, Akiko; Scholl, Claudia; Bansal, Dimple; Zon, Leonard Ira; Fröhling, Stefan; Daley, George Quentin; Gilliland, D. Gary; Mercher, Thomas (Ferrata Storti Foundation (Haematologica), 2010)
      Background: Cdx4 is a homeobox gene essential for normal blood formation during embryonic development in the zebrafish, through activation of posterior Hox genes. However, its role in adult mammalian hematopoiesis has not ...
    • Chromatin Modifying Enzymes as Modulators of Reprogramming 

      Onder, Tamer T.; Kara, Nergis; Sinha, Amit U.; Bernt, Kathrin M.; Mancarci, Ogan. B.; Gupta, Piyush B.; Cherry, Anne Blanche Cresswell; Zhu, Nan; Cahan, Patrick; Unternaehrer, Juli; Lander, Eric Steven; Armstrong, Scott A.; Daley, George Quentin (Nature Publishing Group, 2012)
      Generation of induced pluripotent stem cells (iPSCs) by somatic cell reprogramming involves global epigenetic remodeling. While several proteins are known to regulate chromatin marks associated with the distinct epigenetic ...
    • A comparison of non-integrating reprogramming methods 

      Schlaeger, Thorsten M.; Daheron, Laurence Marie; Brickler, Thomas R; Entwisle, Samuel; Chan, Karrie; Cianci, Amelia; DeVine, Alexander; Ettenger, Andrew; Fitzgerald, Kelly; Godfrey, Michelle; Gupta, Dipti; McPherson, Jade; Malwadkar, Prerana; Gupta, Manav; Bell, Blair; Doi, Akiko; Jung, Namyoung; Li, Xin; Lynes, Maureen M.; Brookes, Emily; Cherry, Anne B C; Demirbas, Didem; Tsankov, Alexander; Zon, Leonard Ira; Rubin, Lee; Feinberg, Andrew P; Meissner, Alexander; Cowan, Chad A.; Daley, George Quentin (Nature Publishing Group, 2014)
      Human induced pluripotent stem cells (hiPSCs1, 2, 3) are useful in disease modeling and drug discovery, and they promise to provide a new generation of cell-based therapeutics. To date there has been no systematic evaluation ...
    • Deconstructing transcriptional heterogeneity in pluripotent stem cells 

      Kumar, Roshan M.; Cahan, Patrick; Shalek, Alex K.; Satija, Rahul; DaleyKeyser, AJay; Li, Hu; Zhang, Jin; Pardee, Keith; Gennert, David; Trombetta, John J.; Ferrante, Thomas C.; Regev, Aviv; Daley, George Q.; Collins, James J. (2014)
      SUMMARY Pluripotent stem cells (PSCs) are capable of dynamic interconversion between distinct substates, but the regulatory circuits specifying these states and enabling transitions between them are not well understood. ...
    • Defining markers and mechanisms of human somatic cell reprogramming 

      Ratanasirintrawoot, Sutheera (2014-02-25)
      Somatic cells can be reprogrammed into induced pluripotent stem (iPS) cells by over expression of the transcription factors OCT4, SOX2, KLF4 and c-MYC. Using serial live cell immunofluorescence imaging of human fibroblasts ...
    • Developmental Maturation within the Hematopoietic System 

      Arora, Natasha (2014-06-06)
      Stem cell biologists creating cells and tissues for therapies, disease modeling, and drug screening have observed that differentiating pluripotent stem cells (PSCs) tend to produce cells at an embryonic stage of development ...
    • Developmental regulation of myeloerythroid progenitor function by the Lin28b–let-7–Hmga2 axis 

      Rowe, R. Grant; Wang, Leo D.; Coma, Silvia; Han, Areum; Mathieu, Ronald; Pearson, Daniel S.; Ross, Samantha; Sousa, Patricia; Nguyen, Phi T.; Rodriguez, Antony; Wagers, Amy J.; Daley, George Q. (The Rockefeller University Press, 2016)
      For appropriate development, tissue and organ system morphogenesis and maturation must occur in synchrony with the overall developmental requirements of the host. Mistiming of such developmental events often results in ...
    • Epoxyeicosatrienoic Acids Enhance Embryonic Haematopoiesis and Adult Marrow Engraftment 

      Li, Pulin; Lahvic, Jamie L.; Binder, Vera; Pugach, Emily K.; Riley, Elizabeth B.; Tamplin, Owen J.; Panigrahy, Dipak; Bowman, Teresa V.; Barrett, Francesca G.; Heffner, Garrett C.; McKinney-Freeman, Shannon; Schlaeger, Thorsten M.; Daley, George Q.; Zeldin, Darryl C.; Zon, Leonard I. (2015)
      Haematopoietic stem and progenitor cell (HSPC) transplant is a widely used treatment for life-threatening conditions including leukemia; however, the molecular mechanisms regulating HSPC engraftment of the recipient niche ...
    • Flow-induced protein kinase A–CREB pathway acts via BMP signaling to promote HSC emergence 

      Kim, Peter Geon; Nakano, Haruko; Das, Partha P.; Chen, Michael J.; Rowe, R. Grant; Chou, Stephanie S.; Ross, Samantha J.; Sakamoto, Kathleen M.; Zon, Leonard I.; Schlaeger, Thorsten M.; Orkin, Stuart H.; Nakano, Atsushi; Daley, George Q. (The Rockefeller University Press, 2015)
      Fluid shear stress promotes the emergence of hematopoietic stem cells (HSCs) in the aorta–gonad–mesonephros (AGM) of the developing mouse embryo. We determined that the AGM is enriched for expression of targets of protein ...
    • Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA 

      Warren, Luigi; Manos, Philip D.; Ahfeldt, Tim; Loh, Yuin-Han; Li, Hu; Lau, Frank; Ebina, Wataru; Mandal, Pankaj Kumar; Smith, Zachary D; Meissner, Alexander; Daley, George Quentin; Brack, Andrew Stephen; Collins, James J.; Cowan, Chad A.; Schlaeger, Thorsten M.; Rossi, Derrick J (Elsevier BV, 2010)
      Clinical application of induced pluripotent stem cells (iPSCs) is limited by the low efficiency of iPSC derivation and the fact that most protocols modify the genome to effect cellular reprogramming. Moreover, safe and ...
    • Human endogenous retrovirus K (HML-2) RNA and protein expression is a marker for human embryonic and induced pluripotent stem cells 

      Fuchs, Nina V; Loewer, Sabine; Daley, George Q; Izsvák, Zsuzsanna; Löwer, Johannes; Löwer, Roswitha (BioMed Central, 2013)
      Background: Malignant human embryonal carcinoma cells (ECCs) rely on similar transcriptional networks as non-malignant embryonic stem cells (ESCs) to control selfrenewal, maintain pluripotency, and inhibit differentiation. ...
    • Impaired intrinsic immunity to HSV-1 in human iPSC-derived TLR3-deficient CNS cells 

      Lafaille, Fabien G; Pessach, Itai M.; Zhang, Shen-Ying; Ciancanelli, Michael J.; Herman, Melina; Abhyankar, Avinash; Ying, Shui-Wang; Keros, Sotirios; Goldstein, Peter A.; Mostoslavsky, Gustavo; Ordovas-Montanes, Jose Manuel; Jouanguy, Emmanuelle; Plancoulaine, Sabine; Tu, Edmund; Elkabetz, Yechiel; Al-Muhsen, Saleh; Tardieu, Marc; Schlaeger, Thorsten M.; Daley, George Quentin; Abel, Laurent; Casanova, Jean-Laurent; Studer, Lorenz; Notarangelo, Luigi D. (2012)
      In the course of primary infection with herpes simplex virus 1 (HSV-1), children with inborn errors of TLR3 immunity are prone to HSV-1 encephalitis (HSE) 1–3. We tested the hypothesis that the pathogenesis of HSE involves ...
    • Interaction of Retinoic Acid and scl Controls Primitive Blood Development 

      de Jong, Jill L. O.; Davidson, Alan; Wang, Yuan; Palis, James; Opara, Praise; Pugach, Emily; Daley, George Quentin; Zon, Leonard Ira (American Society of Hematology, 2010)
      Hematopoietic development during embryogenesis involves the interaction of extrinsic signaling pathways coupled to an intrinsic cell fate that is regulated by cell-specific transcription factors. Retinoic acid (RA) has ...
    • Lin28a/b in the Regulation of Developmental Timing and Tumorigenesis 

      Robinton, Daisy Andre (2016-05-10)
      The heterochronic genes Lin28a/b and let-7 have long been known to play critical roles in the development of organisms from worms to humans. These highly conserved genes have emerged as factors that regulate self-renewal, ...
    • Lineage Regulators Direct BMP and Wnt Pathways to Cell-Specific Programs during Differentiation and Regeneration 

      Trompouki, Eirini; Bowman, Teresa Venezia; Lawton, Lee N.; Fan, Zi Peng; Wu, Dai-Chen; DiBiase, Anthony; Martin, Corey S.; Cech, Jennifer N.; Sessa, Anna K.; Leblanc, Jocelyn L.; Li, Pulin; Durand, Ellen Marie; Mosimann, Christian; Heffner, Garrett C.; Daley, George Quentin; Paulson, Robert F.; Young, Richard A.; Zon, Leonard Ira (Elsevier BV, 2011)
      BMP and Wnt signaling pathways control essential cellular responses through activation of the transcription factors SMAD (BMP) and TCF (Wnt). Here, we show that regeneration of hematopoietic lineages following acute injury ...
    • Multiple mechanisms disrupt the let-7 microRNA family in neuroblastoma 

      Powers, John T; Tsanov, Kaloyan M; Pearson, Daniel S; Roels, Frederik; Spina, Catherine S; Ebright, Richard; Seligson, Marc; de Soysa, Yvanka; Cahan, Patrick; Theiβen, Jessica; Tu, Ho-Chou; Han, Areum; Kurek, Kyle C; LaPier, Grace S; Osborne, Jihan K; Ross, Samantha J; Cesana, Marcella; Collins, James J; Berthold, Frank; Daley, George Q (2016)
      Poor prognosis in neuroblastoma is associated with genetic amplification of MYCN. MYCN is itself a target of let-7, a tumor suppressor family of microRNAs implicated in numerous cancers. LIN28B, an inhibitor of let-7 ...