Browsing by Author "Jacobs, Pieter P."

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Fed-Batch Fermentation of GM-CSF-Producing Glycoengineered Pichia pastoris Under Controlled Specific Growth Rate

Jacobs, Pieter P.; Inan, Mehmet; Festjens, Nele; Haustraete, Jurgen; Van Hecke, Annelies; Contreras, Roland; Meagher, Michael M; Callewaert, Nico (BioMed Central, 2010)

Background: Yeast expression systems with altered N-glycosylation are now available to produce glycoproteins with homogenous, defined N-glycans. However, data on the behaviour of these strains in high cell density cultivation ...
The HAC1 Gene from Pichia Pastoris: Characterization and Effect of its Overexpression on the Production of Secreted, Surface Displayed and Membrane Proteins

Guerfal, Mouna; Ryckaert, Stefan; Jacobs, Pieter P.; Ameloot, Paul; Van Craenenbroeck, Kathleen; Derycke, Riet; Callewaert, Nico (BioMed Central, 2010)

Background: The unfolded protein response (UPR) in eukaryotes upregulates factors that restore ER homeostasis upon protein folding stress and in yeast is activated by a non-conventional splicing of the HAC1 mRNA. The spliced ...