Now showing items 1-7 of 7

    • ARTIST: High-Resolution Genome-Wide Assessment of Fitness Using Transposon-Insertion Sequencing 

      Pritchard, Justin R.; Chao, Michael C.; Abel, Sören; Davis, Brigid M.; Baranowski, Catherine; Zhang, Yanjia J.; Rubin, Eric J.; Waldor, Matthew K. (Public Library of Science, 2014)
      Transposon-insertion sequencing (TIS) is a powerful approach for deciphering genetic requirements for bacterial growth in different conditions, as it enables simultaneous genome-wide analysis of the fitness of thousands ...
    • Compound-gene interaction mapping reveals distinct roles for Staphylococcus aureus teichoic acids 

      Santa Maria, J. P.; Sadaka, A.; Moussa, S; Brown, Stephanie Michelle; Zhang, Yanjia Jason; Rubin, Eric Joseph; Gilmore, Michael S.; Walker, Susan Saks (Proceedings of the National Academy of Sciences, 2014)
      Staphylococcus aureus contains two distinct teichoic acid (TA) polymers, lipoteichoic acid (LTA) and wall teichoic acid (WTA), which are proposed to play redundant roles in regulating cell division. To gain insight into ...
    • Global Assessment of Genomic Regions Required for Growth in Mycobacterium tuberculosis 

      Zhang, Yanjia Jason; Ioerger, Thomas R.; Huttenhower, Curtis; Long, Jarukit E.; Sassetti, Christopher M.; Sacchettini, James C.; Rubin, Eric Joseph (Public Library of Science, 2012)
      Identifying genomic elements required for viability is central to our understanding of the basic physiology of bacterial pathogens. Recently, the combination of high-density mutagenesis and deep sequencing has allowed for ...
    • High-resolution definition of the Vibrio cholerae essential gene set with hidden Markov model–based analyses of transposon-insertion sequencing data 

      Chao, Michael C.; Pritchard, Justin R.; Zhang, Yanjia J.; Rubin, Eric J.; Livny, Jonathan; Davis, Brigid M.; Waldor, Matthew K. (Oxford University Press, 2013)
      The coupling of high-density transposon mutagenesis to high-throughput DNA sequencing (transposon-insertion sequencing) enables simultaneous and genome-wide assessment of the contributions of individual loci to bacterial ...
    • High-Throughput Sequencing Enhanced Phage Display Identifies Peptides That Bind Mycobacteria 

      Ngubane, Nqobile A. C.; Gresh, Lionel; Ioerger, Thomas R.; Sacchettini, James C.; Zhang, Yanjia J.; Rubin, Eric J.; Pym, Alexander; Khati, Makobetsa (Public Library of Science, 2013)
      Bacterial cell wall components have been previously used as infection biomarkers detectable by antibodies. However, it is possible that the surface of the Mycobacterium tuberculosis (M. tb), the causative agent of tuberculosis ...
    • Inflammatory signaling in human Tuberculosis granulomas is spatially organized 

      Marakalala, Mohlopheni J.; Raju, Ravikiran M.; Sharma, Kirti; Zhang, Yanjia J.; Eugenin, Eliseo A.; Prideaux, Brendan; Daudelin, Isaac B.; Chen, Pei-Yu; Booty, Matthew G.; Kim, Jin Hee; Eum, Seok Yong; Via, Laura E.; Behar, Samuel M.; Barry, Clifton E.; Mann, Matthias; Dartois, Véronique; Rubin, Eric J. (2016)
      Granulomas are the pathological hallmark of tuberculosis (TB). However, their function and mechanisms of formation remain poorly understood. To understand the role of granulomas in TB, we analyzed the proteomes of granulomas ...
    • Turning the tide against TB: Remaking ineffective host defenses into mechanisms for tuberculosis control 

      Zhang, Yanjia Jason (2013-10-08)
      Most antibiotics, including the drugs currently used for treating tuberculosis (TB), were first discovered as molecules that inhibit bacterial growth in laboratory culture conditions and later translated to infection models ...