Browsing by Author "Walz, Thomas"

Now showing items 1-17 of 17

    • Biochemical and Structural Studies of Membrane Proteins 

      Wang, Ruiqi Rachel (2012-08-10)
      Membrane proteins live at the interface between a cell and its environment; hence, they play a variety of important physiological roles such as transmembrane transport, signal transduction, and cell adhesion. The importance ...

    • CATCHR and HOPS-CORVET tethering complexes share a similar architecture 

      Chou, Hui-Ting; Dukovski, Danijela; Chambers, Melissa G.; Reinisch, Karin M.; Walz, Thomas (2016)
      We show that the Saccharomyces cerevisiae GARP complex and the Cog1-4 subcomplex of the COG complex, both members of the complexes associated with tethering containing helical rods (CATCHR) family of multisubunit tethering ...

    • Chemical and Cellular Defenses against Foreign Pathogens 

      Lin, Xiaonan (2012-09-07)
      Bacterial and viral infections affect billions of people each year. While bacterial infections are treated by the use of antibiotics, viral infections are eradicated by the immune system. This thesis comprises two parts. ...

    • Effect of Lipid Head Groups on Double-Layered Two-Dimensional Crystals Formed by Aquaporin-0 

      Hite, Richard Kevin; Chiu, Po-Lin; Schuller, Jan Michael; Walz, Thomas (Public Library of Science, 2015)
      Aquaporin-0 (AQP0) is a lens-specific water channel that also forms membrane junctions. Reconstitution of AQP0 with dimyristoyl phosphatidylcholine (DMPC) and E. coli polar lipids (EPL) yielded well-ordered, double-layered ...

    • The Hexamer Structure of the Rift Valley Fever Virus Nucleoprotein Suggests a Mechanism for its Assembly into Ribonucleoprotein Complexes 

      Danek, Eric I.; Luo, Dahai; Wong, Yeehwa; Coutard, Bruno; Lantez, Violaine; Canard, Bruno; Lescar, Julien; Ferron, François; Charrel, Rémi; Li, Zongli; Walz, Thomas (Public Library of Science, 2011)
      Rift Valley fever virus (RVFV), a Phlebovirus with a genome consisting of three single-stranded RNA segments, is spread by infected mosquitoes and causes large viral outbreaks in Africa. RVFV encodes a nucleoprotein (N) ...

    • High-Density Reconstitution of Functional Water Channels into Vesicular and Planar Block Copolymer Membranes 

      Kumar, Manish; Habel, Joachim E. O.; Shen, Yue-xiao; Meier, Wolfgang P.; Walz, Thomas (American Chemical Society, 2012)
      The exquisite selectivity and unique transport properties of membrane proteins can be harnessed for a variety of engineering and biomedical applications if suitable membranes can be produced. Amphiphilic block copolymers ...

    • Huntingtin Facilitates Polycomb Repressive Complex 2 

      Woda, Juliana M.; Song, Ji-Joon; Lloret, Alejandro; Abeyrathne, Priyanka D.; Gregory, Gillian; Lee, Jong-Min; Conlon, Ronald A.; Seong, Ihn Sik; Woo, Caroline; Wheeler, Vanessa Chantal; Walz, Thomas; Kingston, Robert Edward; Gusella, James Francis; MacDonald, Marcy Elizabeth (Oxford University Press, 2009)
      Huntington's disease (HD) is caused by expansion of the polymorphic polyglutamine segment in the huntingtin protein. Full-length huntingtin is thought to be a predominant HEAT repeat α-solenoid, implying a role as a ...

    • Interactions of Lipids with Aquaporin-0 and Other Membrane Proteins 

      Hite, Richard; Tamir, Gonen; Harrison, Stephen; Walz, Thomas (Springer Verlag, 1995)
      The structure of aquaporin-0 (AQP0) has recently been determined by electron crystallography of two-dimensional (2D) crystals and by x-ray crystallography of three-dimensional (3D) crystals. The electron crystallographic ...

    • Lipid-Protein Interactions in Double-Layered Two-Dimensional AQP0 Crystals 

      Gonen, Tamir; Cheng, Yifan; Sliz, Piotr; Hiroaki, Yoko; Fujiyoshi, Yoshinori; Harrison, Stephen; Walz, Thomas (Nature Publishing Group, 2005)
      Lens-specific aquaporin-0 (AQP0) functions as a specific water pore and forms the thin junctions between fibre cells. We describe a 1.9 Å resolution structure of junctional AQP0, determined by electron crystallography of ...

    • Molecular architecture of the complete COG tethering complex 

      Ha, Jun Yong; Chou, Hui-Ting; Ungar, Daniel; Yip, Calvin K.; Walz, Thomas; Hughson, Frederick M. (2016)
      The conserved oligomeric Golgi (COG) complex orchestrates vesicular trafficking to and within the Golgi apparatus. Here, we use negative-stain electron microscopy to elucidate the architecture of the hetero-octameric COG ...

    • Molecular Structure and Dimeric Organization of the Notch Extracellular Domain as Revealed by Electron Microscopy 

      Lake, Robert J.; Middelkoop, Teije C.; Fan, Hua-Ying; Lashuel, Hilal; Kelly, Deborah F.; Artavanis-Tsakonas, Spiro; Walz, Thomas (Public Library of Science, 2010)
      Background: The Notch receptor links cell fate decisions of one cell to that of the immediate cellular neighbor. In humans, malfunction of Notch signaling results in diseases and congenital disorders. Structural information ...

    • Negative Staining and Image Classification – Powerful Tools in Modern Electron Microscopy 

      Ohi, Melanie; Li, Ying; Cheng, Yifan; Walz, Thomas (Biological Procedures Online, 2004)
      Vitrification is the state-of-the-art specimen preparation technique for molecular electron microscopy (EM) and therefore negative staining may appear to be an outdated approach. In this paper we illustrate the specific ...

    • Principles of Membrane Protein Interactions with Annular Lipids Deduced from Aquaporin-0 2D Crystals 

      Hite, Richard K; Li, Zongli; Walz, Thomas (Nature Publishing Group, 2010)
      We have previously described the interactions of aquaporin-0 (AQP0) with dimyristoyl phosphatidylcholine (DMPC) lipids. We have now determined the 2.5 Å structure of AQP0 in two-dimensional (2D) crystals formed with ...

    • Structural analyses of the chromatin remodeling enzymes INO80-C and SWR-C 

      Watanabe, Shinya; Tan, Dongyan; Lakshminarasimhan, Mahadevan; Washburn, Michael P.; Hong, Eun-Jin Erica; Walz, Thomas; Peterson, Craig L. (2015)
      INO80-C and SWR-C are conserved members of a subfamily of ATP-dependent chromatin remodeling enzymes that function in transcription and genome-maintenance pathways. A crucial role for these enzymes is to control chromosomal ...

    • Structural specializations of \(\alpha_4 \beta_7\), an integrin that mediates rolling adhesion 

      Yu, Yamei; Zhu, Jianghai; Mi, Li-Zhi; Walz, Thomas; Sun, Hao; Chen, JianFeng; Springer, Timothy A. (The Rockefeller University Press, 2012)
      The lymphocyte homing receptor integrin \(\alpha_4 \beta_7\) is unusual for its ability to mediate both rolling and firm adhesion. \(\alpha_4 \beta_1\) and \(\alpha_4 \beta_7\) are targeted by therapeutics approved for ...

    • Structural Study of the RIPoptosome Core Reveals a Helical Assembly for Kinase Recruitment 

      Jang, Tae-ho; Zheng, Chao; Li, Jixi; Richards, Claire; Hsiao, Yu-Shan; Walz, Thomas; Wu, Hao; Park, Hyun Ho (American Chemical Society, 2014)
      Receptor interaction protein kinase 1 (RIP1) is a molecular cell-fate switch. RIP1, together with Fas-associated protein with death domain (FADD) and caspase-8, forms the RIPoptosome that activates apoptosis. RIP1 also ...

    • Structure and function of a single-chain, multi-domain long-chain acyl-CoA carboxylase 

      Tran, Timothy H.; Hsiao, Yu-Shan; Jo, Jeanyoung; Chou, Chi-Yuan; Dietrich, Lars E.P.; Walz, Thomas; Tong, Liang (2014)
      Biotin-dependent carboxylases are widely distributed in nature and have important functions in the metabolism of fatty acids, amino acids, carbohydrates, cholesterol and other compounds 1–6. Defective mutations in several ...