Now showing items 1-20 of 20

    • Acute Schistosoma mansoni Infection Increases Susceptibility to Systemic SHIV Clade C Infection in Rhesus Macaques after Mucosal Virus Exposure 

      Chenine, Agnès-Laurence; Shai-Kobiler, Ela; Steele, Lisa N.; Ong, Helena; Augostini, Peter; Song, Ruijiang; Lee, Sandra J.; Autissier, Patrick; Ruprecht, Ruth Margrit; Secor, W. Evan (Public Library of Science, 2008)
      Background: Individuals living in sub-Saharan Africa represent 10% of the world's population but almost 2/3 of all HIV-1/AIDS cases. The disproportionate HIV-1 infection rates in this region may be linked to helminthic ...
    • An Anti-HIV-1 V3 Loop Antibody Fully Protects Cross-Clade and Elicits T-Cell Immunity in Macaques Mucosally Challenged with an R5 Clade C SHIV 

      Hemashettar, Girish; Wang, Wendy; Novembre, Francis J.; Villinger, Francois; Ibegbu, Chris; Patel, Kalpana; Corti, Davide; Agatic, Gloria; Vanzetta, Fabrizia; Bianchi, Siro; Heeney, Jonathan L.; Sallusto, Federica; Lanzavecchia, Antonio; Watkins, Jennifer D; Siddappa, Nagadenahalli B.; Lakhashe, Samir; Humbert, Michael; Sholukh, Anton M; Wong, Yin Ling; Yoon, John C.; Ruprecht, Ruth Margrit (Public Library of Science, 2011)
      Neutralizing antibodies have been shown to protect macaques against SHIV challenge. However, genetically diverse HIV-1 clades have evolved, and a key question left unanswered is whether neutralizing antibodies can confer ...
    • CD1c bypasses lysosomes to present a lipopeptide antigen with 12 amino acids 

      Van Rhijn, Ildiko; De Jong, Annemieke; Vazquez, Jenny; Cheng, Tan-Yun; Barral, Duarte C.; León, Luis; Riese, Richard; Costello, Catherine E.; Porcelli, Steven A.; Briken, Volker; Young, David Stephenson; Young, David C.; Talekar, Rahul Subhash; Brenner, Michael Barry; Katz, Joel Thorp; Ruprecht, Ruth Margrit; O'Connor, Peter B.; Moody, D Branch (The Rockefeller University Press, 2009)
      The recent discovery of dideoxymycobactin (DDM) as a ligand for CD1a demonstrates how a nonribosomal lipopeptide antigen is presented to T cells. DDM contains an unusual acylation motif and a peptide sequence present only ...
    • CD1c Bypasses Lysosomes to Present a Lipopeptide Antigen with 12 Amino Acids 

      De Jong, Annemieke; Vazquez, Jenny; Cheng, Tan-Yun; Barral, Duarte C.; León, Luis; Riese, Richard; Costello, Catherine E.; Porcelli, Steven A.; Briken, Volker; Van Rhijn, Ildiko; Young, David C.; Talekar, Rahul Subhash; Brenner, Michael Barry; Katz, Joel Thorp; Ruprecht, Ruth Margrit; O'Connor, Peter B.; Moody, D Branch (The Rockefeller University Press, 2009)
      The recent discovery of dideoxymycobactin (DDM) as a ligand for CD1a demonstrates how a nonribosomal lipopeptide antigen is presented to T cells. DDM contains an unusual acylation motif and a peptide sequence present only ...
    • Characterization of HIV-1 Subtype C Envelope Glycoproteins From Perinatally Infected Children With Different Courses of Disease 

      Hoffmann, Federico; He, Jun; He, Xiang; Kankasa, Chipepo; West, John T; Mitchell, Charles D; Orti, Guillermo; Wood, Charles; Zhang, Hong; Ruprecht, Ruth Margrit (BioMed Central, 2006)
      Background: The causal mechanisms of differential disease progression in HIV-1 infected children remain poorly defined, and much of the accumulated knowledge comes from studies of subtype B infected individuals. The ...
    • Clade C HIV-1 isolates circulating in Southern Africa exhibit a greater frequency of dicysteine motif-containing Tat variants than those in Southeast Asia and cause increased neurovirulence 

      Rao, Vasudev R; Neogi, Ujjwal; Talboom, Joshua S; Padilla, Ligia; Rahman, Mustafizur; Fritz-French, Cari; Gonzalez-Ramirez, Sandra; Verma, Anjali; Wood, Charles; Ruprecht, Ruth M; Ranga, Udaykumar; Azim, Tasnim; Joska, John; Eugenin, Eliseo; Shet, Anita; Bimonte-Nelson, Heather; Tyor, William R; Prasad, Vinayaka R (BioMed Central, 2013)
      Background: HIV-1 Clade C (Subtype C; HIV-1C) is responsible for greater than 50% of infections worldwide. Unlike clade B HIV-1 (Subtype B; HIV-1B), which is known to cause HIV associated dementia (HAD) in approximately ...
    • Dynamics of Envelope Evolution in Clade C SHIV-Infected Pig-Tailed Macaques during Disease Progression Analyzed by Ultra-Deep Pyrosequencing 

      Tso, For Yue; Tully, Damien C; Gonzalez, Sandra; Quince, Christopher; Ho, On; Polacino, Patricia; Ruprecht, Ruth Margrit; Hu, Shiu-Lok; Wood, Charles (Public Library of Science, 2012)
      Understanding the evolution of the human immunodeficiency virus type 1 (HIV-1) envelope during disease progression can provide tremendous insights for vaccine development, and simian-human immunodeficiency virus (SHIV) ...
    • Evolution of subtype C HIV-1 Env in a slowly progressing Zambian infant 

      Hoffmann, Federico; He, Jun; He, Xiang; Kankasa, Chipepo; West, John T; Orti, Guillermo; Wood, Charles; Zhang, Hong; Ruprecht, Ruth Margrit (BioMed Central, 2005)
      Background: Given the high prevalence of mother to child infection, the development of a better understanding of African subtype C HIV-1 transmission and natural evolution is of significant importance. In this study, we ...
    • Identification and Characterization of a Broadly Cross-Reactive HIV-1 Human Monoclonal Antibody That Binds to Both gp120 and gp41 

      Zhang, Mei-Yun; Yuan, Tingting; Rosa Borges, Andrew; Guenaga, Javier; Yang, Zheng; Wang, Yanping; Li, Yuxing; Polonis, Victoria R.; Pincus, Seth H.; Dimitrov, Dimiter S.; Li, Jingjing; Watkins, Jennifer D; Ruprecht, Ruth Margrit; Wilson, Richard (Public Library of Science, 2012)
      Identification of broadly cross-reactive HIV-1-neutralizing antibodies (bnAbs) may assist vaccine immunogen design. Here we report a novel human monoclonal antibody (mAb), designated m43, which co-targets the gp120 and ...
    • Inducing Cross-Clade Neutralizing Antibodies against HIV-1 by Immunofocusing 

      Humbert, Michael; Rasmussen, Robert Anthony; Ong, Helena; Kaiser, Fabian M. P.; Hu, Shiu-Lok; Ruprecht, Ruth Margrit (Public Library of Science, 2008)
      Background: Although vaccines are important in preventing viral infections by inducing neutralizing antibodies (nAbs), HIV-1 has proven to be a difficult target and escapes humoral immunity through various mechanisms. We ...
    • Isolation of Monoclonal Antibodies with Predetermined Conformational Epitope Specificity 

      Sholukh, Anton M.; Mukhtar, Muhammad Mahmood; Humbert, Michael; Essono, Sosthène S.; Watkins, Jennifer D.; Vyas, Hemant Kumar; Shanmuganathan, Vivekanandan; Hemashettar, Girish; Kahn, Maria; Hu, Shiu-Lok; Montefiori, David C.; Polonis, Victoria R.; Schur, Peter Henry; Ruprecht, Ruth Margrit (Public Library of Science, 2012)
      Existing technologies allow isolating antigen-specific monoclonal antibodies (mAbs) from B cells. We devised a direct approach to isolate mAbs with predetermined conformational epitope specificity, using epitope mimetics ...
    • Live Attenuated Rev-Independent Nef¯SIV Enhances Acquisition of Heterologous SIVsmE660 in Acutely Vaccinated Rhesus Macaques 

      Byrareddy, Siddappa N.; Ayash-Rashkovsky, Mila; Kramer, Victor G.; Lee, Sandra J.; Correll, Mick; Novembre, Francis J.; Villinger, Francois; Johnson, Welkin E.; von Gegerfelt, Agneta; Felber, Barbara K.; Ruprecht, Ruth M. (Public Library of Science, 2013)
      Background: Rhesus macaques (RMs) inoculated with live-attenuated Rev-Independent Nef¯ simian immunodeficiency virus (Rev-Ind Nef¯SIV) as adults or neonates controlled viremia to undetectable levels and showed no signs of ...
    • Live-virus exposure of vaccine-protected macaques alters the anti-HIV-1 antibody repertoire in the absence of viremia 

      Bachler, Barbara C; Humbert, Michael; Lakhashe, Samir K; Rasmussen, Robert A; Ruprecht, Ruth M (BioMed Central, 2013)
      Background: We addressed the question whether live-virus challenges could alter vaccine-induced antibody (Ab) responses in vaccinated rhesus macaques (RMs) that completely resisted repeated exposures to R5-tropic simian-human ...
    • Passive immunization of macaques with polyclonal anti-SHIV IgG against a heterologous tier 2 SHIV: outcome depends on IgG dose 

      Sholukh, Anton M; Byrareddy, Siddappa N; Shanmuganathan, Vivekanandan; Hemashettar, Girish; Lakhashe, Samir K; Rasmussen, Robert A; Watkins, Jennifer D; Vyas, Hemant K; Thorat, Swati; Brandstoetter, Tania; Mukhtar, Muhammad M; Yoon, John K; Novembre, Francis J; Villinger, Francois; Landucci, Gary; Forthal, Donald N; Ratcliffe, Sarah; Tuero, Iskra; Robert-Guroff, Marjorie; Polonis, Victoria R; Bilska, Miroslawa; Montefiori, David C; Johnson, Welkin E; Ertl, Hildegund C; Ruprecht, Ruth M (BioMed Central, 2014)
      Background: A key goal for HIV-1 envelope immunogen design is the induction of cross-reactive neutralizing antibodies (nAbs). As AIDS vaccine recipients will not be exposed to strains exactly matching any immunogens due ...
    • Passive immunization with polyclonal anti-SHIV IgG: partial protection or increased acquisition of heterologous tier 2 SHIV – depending on IgG dose 

      Sholukh, Anton M; Siddappa, NB; Shanmuganathan, V; Lakhashe, Samir; Rasmussen, Robert Anthony; Watkins, Jennifer D; Vyas, Hemant Kumar; Mukhtar, Muhammad Mahmood; Hemashettar, G; Thorat, Swati; Yoon, JK; Villinger, F; Novembre, FJ; Landucci, G; Forthal, DN; Ratcliffe, S; Robert-Guroff, M; Polonis, V; Montefiori, DC; Ertl, HC; Ruprecht, Ruth Margrit (BioMed Central, 2012)
    • Pathogenic Infection of Macaca Nemestrina with a CCR5-tropic Subtype-C Simian-human Immunodeficiency Virus 

      Ho, On; Larsen, Kay; Polacino, Patricia; Li, Yun; Anderson, David; Song, Ruijiang; Ruprecht, Ruth Margrit; Hu, Shiu-Lok (BioMed Central, 2009)
      Background: Although pig-tailed macaques (Macaca nemestrina) have been used in AIDS research for years, less is known about the early immunopathogenic events in this species, as compared to rhesus macaques (Macaca mulatta). ...
    • R5 Clade C SHIV Strains with Tier 1 or 2 Neutralization Sensitivity: Tools to Dissect Env Evolution and to Develop AIDS Vaccines in Primate Models 

      Wassermann, Klemens J.; Song, Ruijiang; Wang, Wendy; Kramer, Victor G.; Novembre, Francis J.; Villinger, François; Else, James G.; Montefiori, David C.; Watkins, Jennifer D; Siddappa, Nagadenahalli B.; Lakhashe, Samir; Santosuosso, Michael Robert; Poznansky, Mark; Rasmussen, Robert Anthony; Ruprecht, Ruth Margrit (Public Library of Science, 2010)
      Background: HIV-1 clade C (HIV-C) predominates worldwide, and anti-HIV-C vaccines are urgently needed. Neutralizing antibody (nAb) responses are considered important but have proved difficult to elicit. Although some current ...
    • R5-SHIV Induces Multiple Defects in T Cell Function during Early Infection of Rhesus Macaques Including Accumulation of T Reg Cells in Lymph Nodes 

      Righi, Elda; Leblanc, Pierre R.; Kodish, Brett; Mylvaganam, Hari N.; Stevceva, Liljana; Hu, Shiu-Lok; Chenine, Agnes-L.; Hovav, Avi-Hai; Unutmaz, Derya; Santosuosso, Michael Robert; Siddappa, Nagadenahalli B.; Ghebremichael, Musie Syum; Ruprecht, Ruth Margrit; Poznansky, Mark; Hill, David E. (Public Library of Science, 2011)
      Background: HIV-1 is a pathogen that T cell responses fail to control. HIV-1gp120 is the surface viral envelope glycoprotein that interacts with CD4 T cells and mediates entry. HIV-1gp120 has been implicated in immune ...
    • SHIV-1157i and Passaged Progeny Viruses Encoding R5 HIV-1 Clade C Env Cause AIDS in Rhesus Monkeys 

      Song, Ruijiang; Ong, Helena; Sharma, Prachi; Chenine, Agnès L; Kramer, Victor G; Xu, Weidong; Else, James G; Novembre, Francis J; Strobert, Elizabeth; O'Neil, Shawn P; Humbert, Michael; Rasmussen, Robert Anthony; Siddappa, Nagadenahalli B.; Ruprecht, Ruth Margrit (BioMed Central, 2008)
      Background: Infection of nonhuman primates with simian immunodeficiency virus (SIV) or chimeric simian-human immunodeficiency virus (SHIV) strains is widely used to study lentiviral pathogenesis, antiviral immunity and the ...
    • Variations in the Biological Functions of HIV-1 Clade C Envelope in a SHIV-Infected Rhesus Macaque during Disease Progression 

      Tso, For Yue; Abrahamyan, Levon; Hu, Shiu-Lok; Ruprecht, Ruth M.; Wood, Charles (Public Library of Science, 2013)
      A better understanding of how the biological functions of the HIV-1 envelope (Env) changes during disease progression may aid the design of an efficacious anti-HIV-1 vaccine. Although studies from patient had provided some ...