Now showing items 1-20 of 40

    • The 15kDa Selenoprotein and Thioredoxin Reductase 1 Promote Colon Cancer by Different Pathways 

      Tsuji, Petra A.; Carlson, Bradley A.; Yoo, Min-Hyuk; Naranjo-Suarez, Salvador; Xu, Xue-Ming; He, Yiwen; Asaki, Esther; Seifried, Harold E.; Reinhold, William C.; Davis, Cindy D.; Gladyshev, Vadim N.; Hatfield, Dolph L. (Public Library of Science, 2015)
      Selenoproteins mediate much of the cancer-preventive properties of the essential nutrient selenium, but some of these proteins have been shown to also have cancer-promoting effects. We examined the contributions of the ...
    • Abrogated Thioredoxin System Causes Increased Sensitivity to TNF-α-Induced Apoptosis via Enrichment of p-ERK 1/2 in the Nucleus 

      Yoo, Min-Hyuk; Carlson, Bradley A.; Gladyshev, Vadim N.; Hatfield, Dolph L. (Public Library of Science, 2013)
      Thioredoxin (Trx) and thioredoxin reductase 1 (TR1) are among the major redox regulators in mammalian cells and have a wide variety of roles, including removal of intracellular reactive oxygen species (ROS) and prevention ...
    • Aging: progressive decline in fitness due to the rising deleteriome adjusted by genetic, environmental, and stochastic processes 

      Gladyshev, Vadim N. (John Wiley and Sons Inc., 2016)
      Summary Different theories posit that aging is caused by molecular damage, genetic programs, continued development, hyperfunction, antagonistic pleiotropy alleles, mutations, trade‐offs, incomplete repair, etc. Here, I ...
    • Analysis of cancer genomes reveals basic features of human aging and its role in cancer development 

      Podolskiy, Dmitriy I.; Lobanov, Alexei V.; Kryukov, Gregory V.; Gladyshev, Vadim N. (Nature Publishing Group, 2016)
      Somatic mutations have long been implicated in aging and disease, but their impact on fitness and function is difficult to assess. Here by analysing human cancer genomes we identify mutational patterns associated with ...
    • Cell culture-based profiling across mammals reveals DNA repair and metabolism as determinants of species longevity 

      Ma, Siming; Upneja, Akhil; Galecki, Andrzej; Tsai, Yi-Miau; Burant, Charles F; Raskind, Sasha; Zhang, Quanwei; Zhang, Zhengdong D; Seluanov, Andrei; Gorbunova, Vera; Clish, Clary B; Miller, Richard A; Gladyshev, Vadim N (eLife Sciences Publications, Ltd, 2016)
      Mammalian lifespan differs by >100 fold, but the mechanisms associated with such longevity differences are not understood. Here, we conducted a study on primary skin fibroblasts isolated from 16 species of mammals and ...
    • Cell Proliferation and Motility Are Inhibited by G1 Phase Arrest in 15-kDa Selenoprotein-Deficient Chang Liver Cells 

      Bang, Jeyoung; Huh, Jang Hoe; Na, Ji-Woon; Lu, Qiao; Carlson, Bradley A.; Tobe, Ryuta; Tsuji, Petra A.; Gladyshev, Vadim N.; Hatfield, Dolph L.; Lee, Byeong Jae (Korean Society for Molecular and Cellular Biology, 2015)
      The 15-kDa selenoprotein (Sep15) is a selenoprotein residing in the lumen of the endoplasmic reticulum (ER) and implicated in quality control of protein folding. Herein, we established an inducible RNAi cell line that ...
    • Characterization of Mammalian Selenoprotein O: A Redox-Active Mitochondrial Protein 

      Han, Seong-Jeong; Lee, Byung Cheon; Yim, Sun Hee; Gladyshev, Vadim N.; Lee, Seung-Rock (Public Library of Science, 2014)
      Selenoproteins exhibit diverse biological functions, most of which are associated with redox control. However, the functions of approximately half of mammalian selenoproteins are not known. One such protein is Selenoprotein ...
    • Characterization of methionine oxidation and methionine sulfoxide reduction using methionine-rich cysteine-free proteins 

      Liang, Xinwen; Kaya, Alaattin; Zhang, Yan; Le, Dung T; Hua, Deame; Gladyshev, Vadim N (BioMed Central, 2012)
      Background: Methionine (Met) residues in proteins can be readily oxidized by reactive oxygen species to Met sulfoxide (MetO). MetO is a promising physiological marker of oxidative stress and its inefficient repair by MetO ...
    • Composition and Evolution of the Vertebrate and Mammalian Selenoproteomes 

      Mariotti, Marco; Ridge, Perry G.; Zhang, Yan; Lobanov, Alexei; Pringle, Thomas H.; Guigo, Roderic; Hatfield, Dolph L.; Gladyshev, Vadim (Public Library of Science, 2012)
      Background: Selenium is an essential trace element in mammals due to its presence in proteins in the form of selenocysteine (Sec). Human genome codes for 25 Sec-containing protein genes, and mouse and rat genomes for 24. ...
    • Convergent evolution of marine mammals is associated with distinct substitutions in common genes 

      Zhou, Xuming; Seim, Inge; Gladyshev, Vadim N. (Nature Publishing Group, 2015)
      Phenotypic convergence is thought to be driven by parallel substitutions coupled with natural selection at the sequence level. Multiple independent evolutionary transitions of mammals to an aquatic environment offer an ...
    • Differences in Redox Regulatory Systems in Human Lung and Liver Tumors Suggest Different Avenues for Therapy 

      Tobe, Ryuta; Carlson, Bradley A.; Tsuji, Petra A.; Lee, Byeong Jae; Gladyshev, Vadim N.; Hatfield, Dolph L. (MDPI, 2015)
      A common characteristic of many cancer cells is that they suffer from oxidative stress. They, therefore, require effective redox regulatory systems to combat the higher levels of reactive oxygen species that accompany ...
    • Diversity of Protein and mRNA Forms of Mammalian Methionine Sulfoxide Reductase B1 Due to Intronization and Protein Processing 

      Liang, Xinwen; Fomenko, Dmitri E.; Hua, Deame; Kaya, Alaattin; Gladyshev, Vadim (Public Library of Science, 2010)
      Background: Methionine sulfoxide reductases (Msrs) are repair enzymes that protect proteins from oxidative stress by catalyzing stereospecific reduction of oxidized methionine residues. MsrB1 is a selenocysteine-containing ...
    • DNA repair in species with extreme lifespan differences 

      MacRae, Sheila L.; Croken, Matthew McKnight; Calder, R.B.; Aliper, Alexander; Milholland, Brandon; White, Ryan R.; Zhavoronkov, Alexander; Gladyshev, Vadim N.; Seluanov, Andrei; Gorbunova, Vera; Zhang, Zhengdong D.; Vijg, Jan (Impact Journals LLC, 2015)
      Differences in DNA repair capacity have been hypothesized to underlie the great range of maximum lifespans among mammals. However, measurements of individual DNA repair activities in cells and animals have not substantiated ...
    • Evidence that mutation accumulation does not cause aging in Saccharomyces cerevisiae 

      Kaya, Alaattin; Lobanov, Alexei V; Gladyshev, Vadim N (BlackWell Publishing Ltd, 2014)
      The concept that mutations cause aging phenotypes could not be directly tested previously due to inability to identify age-related mutations in somatic cells and determine their impact on organismal aging. Here, we subjected ...
    • The First International Mini-Symposium on Methionine Restriction and Lifespan 

      Ables, Gene P.; Brown-Borg, Holly M.; Buffenstein, Rochelle; Church, Christopher D.; Elshorbagy, Amany K.; Gladyshev, Vadim N.; Huang, Tsang-hai; Miller, Richard A.; Mitchell, James R.; Richie, John P.; Rogina, Blanka; Stipanuk, Martha H.; Orentreich, David S.; Orentreich, Norman (Frontiers Media S.A., 2014)
      It has been 20 years since the Orentreich Foundation for the Advancement of Science, under the leadership Dr. Norman Orentreich, first reported that low methionine (Met) ingestion by rats extends lifespan (Orentreich et ...
    • Gene expression defines natural changes in mammalian lifespan 

      Fushan, Alexey A; Turanov, Anton A; Lee, Sang-Goo; Kim, Eun Bae; Lobanov, Alexei V; Yim, Sun Hee; Buffenstein, Rochelle; Lee, Sang-Rae; Chang, Kyu-Tae; Rhee, Hwanseok; Kim, Jong-So; Yang, Kap-Seok; Gladyshev, Vadim N (BlackWell Publishing Ltd, 2015)
      Mammals differ more than 100-fold in maximum lifespan, which can be altered in either direction during evolution, but the molecular basis for natural changes in longevity is not understood. Divergent evolution of mammals ...
    • Genome analysis reveals insights into physiology and longevity of the Brandt’s bat Myotis brandtii 

      Seim, Inge; Fang, Xiaodong; Xiong, Zhiqiang; Lobanov, Alexey V.; Huang, Zhiyong; Ma, Siming; Feng, Yue; Turanov, Anton A.; Zhu, Yabing; Lenz, Tobias L.; Gerashchenko, Maxim V.; Fan, Dingding; Hee Yim, Sun; Yao, Xiaoming; Jordan, Daniel; Xiong, Yingqi; Ma, Yong; Lyapunov, Andrey N.; Chen, Guanxing; Kulakova, Oksana I.; Sun, Yudong; Lee, Sang-Goo; Bronson, Roderick T.; Moskalev, Alexey A.; Sunyaev, Shamil R.; Zhang, Guojie; Krogh, Anders; Wang, Jun; Gladyshev, Vadim N. (Nature Pub. Group, 2013)
      Bats account for one-fifth of mammalian species, are the only mammals with powered flight, and are among the few animals that echolocate. The insect-eating Brandt’s bat (Myotis brandtii) is the longest-lived bat species ...
    • Glutathione peroxidase 4 and vitamin E cooperatively prevent hepatocellular degeneration 

      Carlson, Bradley A.; Tobe, Ryuta; Yefremova, Elena; Tsuji, Petra A.; Hoffmann, Victoria J.; Schweizer, Ulrich; Gladyshev, Vadim N.; Hatfield, Dolph L.; Conrad, Marcus (Elsevier, 2016)
      The selenoenzyme glutathione peroxidase 4 (Gpx4) is an essential mammalian glutathione peroxidase, which protects cells against detrimental lipid peroxidation and governs a novel form of regulated necrotic cell death, ...
    • HIF-Independent Regulation of Thioredoxin Reductase 1 Contributes to the High Levels of Reactive Oxygen Species Induced by Hypoxia 

      Naranjo-Suarez, Salvador; Carlson, Bradley A.; Tsuji, Petra A.; Yoo, Min-Hyuk; Gladyshev, Vadim; Hatfield, Dolph L. (Public Library of Science, 2012)
      Cellular adaptation to hypoxic conditions mainly involves transcriptional changes in which hypoxia inducible factors (HIFs) play a critical role. Under hypoxic conditions, HIF protein is stabilized due to inhibition of the ...
    • Identification of Thioredoxin Glutathione Reductase Inhibitors That Kill Cestode and Trematode Parasites 

      Ross, Fabiana; Hernández, Paola; Porcal, Williams; López, Gloria V.; Cerecetto, Hugo; González, Mercedes; Basika, Tatiana; Carmona, Carlos; Fló, Martín; Maggioli, Gabriela; Bonilla, Mariana; Boiani, Mariana; Salinas, Gustavo; Gladyshev, Vadim (Public Library of Science, 2012)
      Parasitic flatworms are responsible for serious infectious diseases that affect humans as well as livestock animals in vast regions of the world. Yet, the drug armamentarium available for treatment of these infections is ...