Now showing items 1-6 of 6

    • Advances in Phage-Assisted Continuous Evolution and Application to Overcoming Bioinsecticide Resistance 

      Badran, Ahmed (2016-05-20)
      The Bacillus thuringiensis δ-endotoxins (Bt toxins) are widely used insecticidal proteins in engineered crops that provide agricultural, economic, and environmental benefits, constituting a substantial and increasingly ...
    • Continuous directed evolution of DNA-binding proteins to improve TALEN specificity 

      Hubbard, Basil P.; Badran, Ahmed H.; Zuris, John A.; Guilinger, John P.; Davis, Kevin M.; Chen, Liwei; Tsai, Shengdar Q.; Sander, Jeffry D.; Joung, J. Keith; Liu, David R. (2015)
      Nucleases containing programmable DNA-binding domains can alter the genomes of model organisms and have the potential to become human therapeutics. Here we present DNA-binding phage-assisted continuous evolution (DB-PACE) ...
    • Continuous evolution of B. thuringiensis toxins overcomes insect resistance 

      Badran, Ahmed H.; Guzov, Victor M.; Huai, Qing; Kemp, Melissa M.; Vishwanath, Prashanth; Kain, Wendy; Nance, Autumn M.; Evdokimov, Artem; Moshiri, Farhad; Turner, Keith H.; Wang, Ping; Malvar, Thomas; Liu, David R. (2016)
      The Bacillus thuringiensis δ-endotoxins (Bt toxins) are widely used insecticidal proteins in engineered crops that provide agricultural, economic, and environmental benefits. The development of insect resistance to Bt ...
    • Development of potent in vivo mutagenesis plasmids with broad mutational spectra 

      Badran, Ahmed H.; Liu, David R. (Nature Pub. Group, 2015)
      Methods to enhance random mutagenesis in cells offer advantages over in vitro mutagenesis, but current in vivo methods suffer from a lack of control, genomic instability, low efficiency and narrow mutational spectra. Using ...
    • In vivo continuous directed evolution 

      Badran, Ahmed Hussein; Liu, David Ruchien (Elsevier BV, 2015)
      The development and application of methods for the laboratory evolution of biomolecules has rapidly progressed over the last few decades. Advancements in continuous microbe culturing and selection design have facilitated ...
    • Negative selection and stringency modulation in phage-assisted constinuous evolution 

      Carlson, Jacob C.; Badran, Ahmed H.; Guggiana-Nilo, Drago A.; Liu, David R. (2014)
      Phage-assisted continuous evolution (PACE) uses a modified filamentous bacteriophage life cycle to dramatically accelerate laboratory evolution experiments. In this work we expand the scope and capabilities of the PACE ...