Browsing by Author "Shalek, Alexander"

Now showing items 1-4 of 4

    • Integrated Single-Cell Analysis of Multicellular Immune Dynamics during Hyper-Acute HIV-1 Infection 

      Kazer, Samuel W.; Aicher, Toby P.; Muema, Daniel M.; Carroll, Shaina L.; Ordovas-Montanes, Jose; Maio, Vincent M.; Tu, Andy A.; Ziegler, Carly G.K.; Nyquist, Sarah K.; Wong, Emily; Ismail, Nasreen; Dong, Mary; Moodley, Amber; Berger Leighton, Bonnie; Love, J. Christopher; Dong, Krista L.; Leslie, Alasdair; Ndhlovu, Zaza; Ndung'u, Thumbi; Walker, Bruce; Shalek, Alexander (Cold Spring Harbor Laboratory, 2020-03-23)
      Cellular immunity is critical for controlling intracellular pathogens, but the dynamics and cooperativity of the evolving host response to infection are not well defined. Here, we apply single-cell RNA-sequencing to ...

    • Nanowire-Mediated Delivery Enables Functional Interrogation of Primary Immune Cells: Application to the Analysis of Chronic Lymphocytic Leukemia 

      Shalek, Alexander Kann; Gaublomme, Jellert Thomas; Wang, Lili; Yosef, Nir; Chevrier, Nicolas; Andersen, Mette S.; Robinson, Jacob T.; Pochet, Nathalie; Neuberg, Donna S.; Gertner, Rona S.; Amit, Ido; Brown, Jennifer Ruth; Hacohen, Nir; Regev, Aviv; Wu, Cathy Ju-Ying; Park, Hongkun (American ChemicalSociety, 2012)
      A circuit level understanding of immune cells and hematological cancers has been severely impeded by a lack of techniques that enable intracellular perturbation without significantly altering cell viability and function. ...

    • Prevention of tuberculosis in macaques after intravenous BCG immunization 

      Darrah, Patricia A.; Zeppa, Joseph J.; Maiello, Pauline; Hackney, Joshua A.; Wadsworth, Marc H.; Hughes, Travis K.; Pokkali, Supriya; Swanson, Phillip A.; Grant, Nicole L.; Rodgers, Mark A.; Kamath, Megha; Causgrove, Chelsea M.; Laddy, Dominick J.; Bonavia, Aurelio; Casimiro, Danilo; Lin, Philana Ling; Klein, Edwin; White, Alexander G.; Scanga, Charles A.; Shalek, Alexander; Roederer, Mario; Flynn, JoAnne L.; Seder, Robert A.; Wadsworth, Marc (Springer Science and Business Media LLC, 2020-01-01)
      Mycobacterium tuberculosis (Mtb) is the leading cause of death from infection worldwide1. The only available vaccine, BCG (Bacillus Calmette–Guérin), is given intradermally and has variable efficacy against pulmonary ...

    • Single-cell RNA-seq highlights intratumoral heterogeneity in primary glioblastoma 

      Patel, Anoop Premswaroop; Tirosh, I.; Trombetta, J. J.; Shalek, Alexander Kann; Gillespie, S. M.; Wakimoto, Hiroaki; Cahill, Daniel P.; Nahed, Brian Vala; Curry, William Thomas; Martuza, Robert Lawrence; Louis, David N.; Rozenblatt-Rosen, O.; Suva, Mario Luca; Regev, A.; Bernstein, Bradley E. (American Association for the Advancement of Science (AAAS), 2014)
      Human cancers are complex ecosystems composed of cells with distinct phenotypes, genotypes and epigenetic states, but current models do not adequately reflect tumor composition in patients. We used single cell RNA-seq to ...