Browsing by Author "Byrne, Susan M"

Now showing items 1-4 of 4

    • Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells 

      Byrne, Susan M.; Ortiz, Luis; Mali, Prashant; Aach, John; Church, George M. (Oxford University Press, 2015)
      Sequence-specific nucleases such as TALEN and the CRISPR/Cas9 system have so far been used to disrupt, correct or insert transgenes at precise locations in mammalian genomes. We demonstrate efficient ‘knock-in’ targeted ...

    • On the design of clone-based haplotyping 

      Lo, Christine; Liu, Rui; Lee, Jehyuk; Robasky, Kimberly; Byrne, Susan; Lucchesi, Carolina; Aach, John; Church, George; Bafna, Vineet; Zhang, Kun (BioMed Central, 2013)
      Background: Haplotypes are important for assessing genealogy and disease susceptibility of individual genomes, but are difficult to obtain with routine sequencing approaches. Experimental haplotype reconstruction based on ...

    • Optimization of scarless human stem cell genome editing 

      Yang, Luhan; Guell, Marc; Byrne, Susan; Yang, Joyce L.; De Los Angeles, Alejandro; Mali, Prashant; Aach, John; Kim-Kiselak, Caroline; Briggs, Adrian W; Rios, Xavier; Huang, Po-Yi; Daley, George; Church, George (Oxford University Press, 2013)
      Efficient strategies for precise genome editing in human-induced pluripotent cells (hiPSCs) will enable sophisticated genome engineering for research and clinical purposes. The development of programmable sequence-specific ...

    • Rapid neurogenesis through transcriptional activation in human stem cells 

      Busskamp, Volker; Lewis, Nathan E; Guye, Patrick; Ng, Alex HM; Shipman, Seth L; Byrne, Susan M; Sanjana, Neville E; Murn, Jernej; Li, Yinqing; Li, Shangzhong; Stadler, Michael; Weiss, Ron; Church, George M (Blackwell Publishing Ltd, 2014)
      Advances in cellular reprogramming and stem cell differentiation now enable ex vivo studies of human neuronal differentiation. However, it remains challenging to elucidate the underlying regulatory programs because ...