Now showing items 1-11 of 11

    • Analysis of Mice Lacking DNaseI Hypersensitive Sites at the 5′ End of the IgH Locus 

      Perlot, Thomas; Pawlitzky, Inka; Manis, John P.; Zarrin, Ali A.; Brodeur, Peter H.; Alt, Frederick W. (Public Library of Science, 2010)
      The 5′ end of the IgH locus contains a cluster of DNaseI hypersensitive sites, one of which (HS1) was shown to be pro-B cell specific and to contain binding sites for the transcription factors PU.1, E2A, and Pax5. These ...
    • Complex Breakpoints and Template Switching Associated with Non-canonical Termination of Homologous Recombination in Mammalian Cells 

      Hartlerode, Andrea J.; Willis, Nicholas A.; Rajendran, Anbazhagan; Manis, John P.; Scully, Ralph (Public Library of Science, 2016)
      A proportion of homologous recombination (HR) events in mammalian cells resolve by “long tract” gene conversion, reflecting copying of several kilobases from the donor sister chromatid prior to termination. Cells lacking ...
    • Defective lymphoid organogenesis underlies the immune deficiency caused by a heterozygous S32I mutation in IκBα 

      Mooster, Jana L.; Le Bras, Severine; Massaad, Michel J.; Jabara, Haifa; Yoon, Juhan; Galand, Claire; Heesters, Balthasar A.; Burton, Oliver T.; Mattoo, Hamid; Manis, John; Geha, Raif S. (The Rockefeller University Press, 2015)
      Patients with ectodermal dysplasia with immunodeficiency (ED-ID) caused by mutations in the inhibitor of NF-κB α (IκBα) are susceptible to severe recurrent infections, despite normal T and B cell numbers and intact in vitro ...
    • DOCK8 Functions as an Adaptor that Links TLR–MyD88 Signaling to B Cell Activation 

      Rauter, Ingrid; Recher, Mike; Wakim, Rima; Dbaibo, Ghassan; Dasouki, Majed; Barlan, Isil; Baris, Safa; Kutukculer, Necil; Ochs, Hans; Plebani, Alessandro; Kanariou, Maria; Lefranc, Gerard; Reisli, Ismail; Fitzgerald, Katerine; Golenbock, Douglas; Keles, Sevgi; Ceja, Reuben; Jabara, Haifa Halim; McDonald, Douglas Ray; Janssen, Erin Margaret; Massaad, Michel; Ramesh, Narayanaswamy; Borzutzky, Arturo; Benson, Halli Louise; Schneider, Lynda C.; Baxi, Sachin; Notarangelo, Luigi D.; Al-Herz, Waleed; Manis, John P.; Chatila, Talal Amine; Geha, Raif Salim (Nature Publishing Group, 2012)
      DOCK8 and MyD88 have been implicated in serologic memory. Here we report antibody responses were impaired and \(CD27^+\) memory B cells were severely reduced in DOCK8-deficient patients. Toll-like receptor 9 (TLR9)- but ...
    • Immature B cells preferentially switch to IgE with increased direct Sμ to Sε recombination 

      Wesemann, Duane Robert; Magee, Jennifer; Boboila, Cristian; Calado, Dinis Pedro; Gallagher, Michael P.; Portuguese, Andrew J.; Manis, John P.; Zhou, Xiaolong; Recher, Mike; Rajewsky, Klaus; Notarangelo, Luigi D.; Alt, Frederick W. (The Rockefeller University Press, 2011)
      Immunoglobulin heavy chain (IgH) class-switch recombination (CSR) replaces initially expressed Cμ (IgM) constant regions (C(H)) exons with downstream C(H) exons. Stimulation of B cells with anti-CD40 plus interleukin-4 ...
    • Investigating Mechanisms of DNA Double Strand Break Joining of Switch Regions During IgH Class Switch Recombination 

      Panchakshari, Rohit (2016-05-19)
      During B cell development, RAG endonuclease cleaves immunoglobulin heavy chain (IgH) V, D, and J gene segments and orchestrates their fusion as deletional events that assemble a V(D)J exon in the same transcriptional ...
    • Leucine-rich repeat containing 8A (LRRC8A) is essential for T lymphocyte development and function 

      Kumar, Lalit; Chou, Janet; Yee, Christina S.K.; Borzutzky, Arturo; Vollmann, Elisabeth H.; von Andrian, Ulrich H.; Park, Shin-Young; Hollander, Georg; Manis, John P.; Poliani, P. Luigi; Geha, Raif S. (The Rockefeller University Press, 2014)
      Lrrc8a is a ubiquitously expressed gene that encodes a leucine-rich repeat (LRR)–containing protein detected at higher levels on the surface of thymocytes than on other immune cells. We generated Lrrc8a−/− mice to investigate ...
    • Oncogenic transformation in the absence of Xrcc4 targets peripheral B cells that have undergone editing and switching 

      Wang, Jing; Alt, Frederick W.; Gostissa, Monica; Datta, Abhishek; Murphy, Michael C.; Alimzhanov, Marat B.; Coakley, Kristen M.; Rajewsky, Klaus; Manis, John P.; Yan, Catherine T. (The Rockefeller University Press, 2008)
      Nonhomologous end-joining (NHEJ) repairs DNA double-strand breaks (DSBs) during V(D)J recombination in developing lymphocytes and during immunoglobulin (Ig) heavy chain (IgH) class switch recombination (CSR) in peripheral ...
    • Orientation-Specific Joining of AID-initiated DNA Breaks Promotes Antibody Class Switching 

      Dong, Junchao; Panchakshari, Rohit A.; Zhang, Tingting; Zhang, Yu; Hu, Jiazhi; Volpi, Sabrina A.; Meyers, Robin M.; Ho, Yu-Jui; Du, Zhou; Robbiani, Davide F.; Meng, Feilong; Gostissa, Monica; Nussenzweig, Michel C.; Manis, John P.; Alt, Frederick W. (2015)
      During B cell development, RAG endonuclease cleaves immunoglobulin heavy chain (IgH) V, D, and J gene segments and orchestrates their fusion as deletional events that assemble a V(D)J exon in the same transcriptional ...
    • Rapid generation of novel models of RAG1 deficiency by CRISPR/Cas9-induced mutagenesis in murine zygotes 

      de Bruin, Lisa Ott; Yang, Wei; Capuder, Kelly; Lee, Yu Nee; Antolini, Maddalena; Meyers, Robin; Gellert, Martin; Musunuru, Kiran; Manis, John; Notarangelo, Luigi (Impact Journals LLC, 2016)
      Mutations in the Recombination Activating Gene 1 (RAG1) can cause a wide variety of clinical and immunological phenotypes in humans, ranging from absence of T and B lymphocytes to occurrence of autoimmune manifestations ...
    • SOCS3 Protein Developmentally Regulates the Chemokine Receptor CXCR4-FAK Signaling Pathway during B Lymphopoiesis 

      Le, Yi; Zhu, Bing-Mei; Harley, Brendan; Park, Shin-Young; Kobayashi, Takashi; Manis, John P.; Luo, Hongbo; Yoshimura, Akihiko; Hennighausen, Lothar; Silberstein, Leslie E. (Elsevier BV, 2007)
      The chemokine CXCL12 induces prolonged focal adhesion kinase (FAK) phosphorylation and sustained proadhesive responses in progenitor bone-marrow (BM) B cells, but not in mature peripheral B cells. Here we demonstrate that ...