Now showing items 1-20 of 22

    • Adjuvant-carrying synthetic vaccine particles augment the immune response to encapsulated antigen and exhibit strong local immune activation without inducing systemic cytokine release 

      Ilyinskii, Petr O.; Roy, Christopher J.; O’Neil, Conlin P.; Browning, Erica A.; Pittet, Lynnelle A.; Altreuter, David H.; Alexis, Frank; Tonti, Elena; Shi, Jinjun; Basto, Pamela A.; Iannacone, Matteo; Radovic-Moreno, Aleksandar F.; Langer, Robert S.; Farokhzad, Omid Cameron; von Andrian, Ulrich H.; Johnston, Lloyd P.M.; Kishimoto, Takashi Kei (Elsevier BV, 2014)
      Augmentation of immunogenicity can be achieved by particulate delivery of an antigen and by its co-administration with an adjuvant. However, many adjuvants initiate strong systemic inflammatory reactions in vivo, leading ...
    • Antigen-specific NK cell memory in rhesus macaques 

      Reeves, R. Keith; Li, Haiying; Jost, Stephanie; Blass, Eryn; Li, Hualin; Schafer, Jamie L.; Varner, Valerie; Manickam, Cordelia; Eslamizar, Leila; Altfeld, Marcus; von Andrian, Ulrich H.; Barouch, Dan H. (2015)
      Natural killer (NK) cells have traditionally been considered nonspecific components of innate immunity, but recent studies have shown features of antigen-specific memory in murine NK cells. However, it has remained unclear ...
    • Bilateral Nerve Alterations in a Unilateral Experimental Neurotrophic Keratopathy Model: A Lateral Conjunctival Approach for Trigeminal Axotomy 

      Yamaguchi, Takefumi; Turhan, Aslihan; Harris, Deshea L.; Hu, Kai; Prüss, Harald; von Andrian, Ulrich; Hamrah, Pedram (Public Library of Science, 2013)
      To study bilateral nerve changes in a newly developed novel mouse model for neurotrophic keratopathy by approaching the trigeminal nerve from the lateral fornix. Surgical axotomy of the ciliary nerve of the trigeminal nerve ...
    • Distamycin A inhibits HMGA1-binding to the P-selectin promoter and attenuates lung and liver inflammation during murine endotoxemia 

      Lopez-Guzman, Silvia; Riascos, Dario F.; Macias, Alvaro A.; Layne, Matthew D.; Harris, Cailin; Reeves, Raymond; Baron, Rebecca Marlene; Cheng, Guiying; Chung, Su Wol; Von Andrian, Ulrich H.; Perrella, Mark A. (Public Library of Science, 2010)
      Background: The architectural transcription factor High Mobility Group-A1 (HMGA1) binds to the minor groove of AT-rich DNA and forms transcription factor complexes (“enhanceosomes”) that upregulate expression of select ...
    • A Dual Role for Corneal Dendritic Cells in Herpes Simplex Keratitis: Local Suppression of Corneal Damage and Promotion of Systemic Viral Dissemination 

      Hu, Kai; Harris, Deshea L.; Yamaguchi, Takefumi; von Andrian, Ulrich H.; Hamrah, Pedram (Public Library of Science, 2015)
      The cornea is the shield to the foreign world and thus, a primary site for peripheral infections. However, transparency and vision are incompatible with inflammation and scarring that may result from infections. Thus, the ...
    • Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells 

      Sharei, Armon; Trifonova, Radiana; Jhunjhunwala, Siddharth; Hartoularos, George C.; Eyerman, Alexandra T.; Lytton-Jean, Abigail; Angin, Mathieu; Sharma, Siddhartha; Poceviciute, Roberta; Mao, Shirley; Heimann, Megan; Liu, Sophia; Talkar, Tanya; Khan, Omar F.; Addo, Marylyn; von Andrian, Ulrich H.; Anderson, Daniel G.; Langer, Robert; Lieberman, Judy; Jensen, Klavs F. (Public Library of Science, 2015)
      Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery ...
    • HIV-infected T cells are migratory vehicles for viral dissemination 

      Murooka, Thomas; Deruaz, Maud; Marangoni, Francesco; Vrbanac, Vladimir D.; Seung, Edward N.; Von Andrian, Ulrich H.; Tager, Andrew Martin; Luster, Andrew David; Mempel, Thorsten Roman (2012)
      After host entry through mucosal surfaces, HIV-1 disseminates to lymphoid tissues to establish a generalized infection of the immune system. The mechanisms by which this virus spreads among permissive target cells locally ...
    • Insights into Vibrio cholerae Intestinal Colonization from Monitoring Fluorescently Labeled Bacteria 

      Millet, Yves A.; Alvarez, David; Ringgaard, Simon; von Andrian, Ulrich H.; Davis, Brigid M.; Waldor, Matthew K. (Public Library of Science, 2014)
      Vibrio cholerae, the agent of cholera, is a motile non-invasive pathogen that colonizes the small intestine (SI). Most of our knowledge of the processes required for V. cholerae intestinal colonization is derived from ...
    • Leucine-rich repeat containing 8A (LRRC8A) is essential for T lymphocyte development and function 

      Kumar, Lalit; Chou, Janet; Yee, Christina S.K.; Borzutzky, Arturo; Vollmann, Elisabeth H.; von Andrian, Ulrich H.; Park, Shin-Young; Hollander, Georg; Manis, John P.; Poliani, P. Luigi; Geha, Raif S. (The Rockefeller University Press, 2014)
      Lrrc8a is a ubiquitously expressed gene that encodes a leucine-rich repeat (LRR)–containing protein detected at higher levels on the surface of thymocytes than on other immune cells. We generated Lrrc8a−/− mice to investigate ...
    • Molecular Mechanisms of CD8+ T Cell Differentiation 

      Godec, Jernej (2016-04-25)
      CD8+ T cells are a crucial component of the adaptive immune system and are required for optimal protection from most pathogens and cancer. They function by secreting pro-inflammatory cytokines and by directly eliminating ...
    • A mucosal vaccine against Chlamydia trachomatis generates two waves of protective memory T cells 

      Stary, G; Olive, A; Radovic-Moreno, A. F.; Gondek, D.; Alvarez, David; Basto, P. A.; Perro, M.; Vrbanac, V. D.; Tager, Andrew Martin; Shi, Jinjun; Yethon, J. A.; Farokhzad, Omid Cameron; Langer, Robert S.; Starnbach, Michael N.; von Andrian, Ulrich H. (American Association for the Advancement of Science (AAAS), 2015)
      Genital Chlamydia trachomatis (Ct) infection induces protective immunity that depends on interferon-γ producing CD4 T-cells. By contrast, mucosal exposure to ultraviolet light (UV)-inactivated Ct (UV-Ct) generated regulatory ...
    • Myeloid-Specific Deletion of Tumor Suppressor PTEN Augments Neutrophil Transendothelial Migration during Inflammation 

      Sarraj, Bara; Massberg, Steffen; Li, Yitang; Kasorn, Anongnard; Subramanian, Kulandayan; Loison, Fabien; Silberstein, Leslie E.; von Andrian, Ulrich H.; Luo, Hongbo (American Association of Immunologists, 2014-11-04)
      Phosphatidylinositol 3,4,5-trisphosphate \((PIP_3)\) is a second messenger that is involved in a number of cell activities including cell growth, proliferation, and motility. \(PIP_3\) is produced by PI3K and regulated by ...
    • A Near-Infrared Cell Tracker Reagent for Multiscopic In Vivo Imaging and Quantification of Leukocyte Immune Responses 

      Berger, Cedric R.; Figueiredo, Jose-Luiz; Swirski, Filip K.; Mempel, Thorsten Roman; Von Andrian, Ulrich H.; Pittet, Mikael J.; Weissleder, Ralph (Public Library of Science, 2007)
      The complexity of the tumor microenvironment necessitates that cell behavior is studied in a broad, multi-scale context. Although tomographic and microscopy-based far and near infrared fluorescence (NIRF, >650 nm) imaging ...
    • Neutrophil Responses to Sterile Implant Materials 

      Jhunjhunwala, Siddharth; Aresta-DaSilva, Stephanie; Tang, Katherine; Alvarez, David; Webber, Matthew J.; Tang, Benjamin C.; Lavin, Danya M.; Veiseh, Omid; Doloff, Joshua C.; Bose, Suman; Vegas, Arturo; Ma, Minglin; Sahay, Gaurav; Chiu, Alan; Bader, Andrew; Langan, Erin; Siebert, Sean; Li, Jie; Greiner, Dale L.; Newburger, Peter E.; von Andrian, Ulrich H.; Langer, Robert; Anderson, Daniel G. (Public Library of Science, 2015)
      In vivo implantation of sterile materials and devices results in a foreign body immune response leading to fibrosis of implanted material. Neutrophils, one of the first immune cells to be recruited to implantation sites, ...
    • Nociceptive Sensory Neurons Drive Interleukin-23 Mediated Psoriasiform Skin Inflammation 

      Riol-Blanco, Lorena; Ordovas-Montanes, Jose; Perro, Mario; Naval, Elena; Thiriot, Aude; Alvarez, David; Wood, John N.; von Andrian, Ulrich H. (2014)
      The skin has a dual function as a barrier and a sensory interface between the body and the environment. To protect against invading pathogens, the skin harbors specialized immune cells, including dermal dendritic cells ...
    • A novel role of sphingosine 1-phosphate receptor S1pr1 in mouse thrombopoiesis 

      Zhang, Lin; Orban, Martin; Lorenz, Michael; Barocke, Verena; Braun, Daniela Anne; Urtz, Nicole; Schulz, Christian; von Brühl, Marie-Luise; Tirniceriu, Anca; Gaertner, Florian; Proia, Richard L.; Graf, Thomas; Bolz, Steffen-Sebastian; Montanez, Eloi; Prinz, Marco; Müller, Alexandra; von Baumgarten, Louisa; Billich, Andreas; Sixt, Michael; Fässler, Reinhard; Von Andrian, Ulrich H.; Junt, Tobias; Massberg, Steffen (The Rockefeller University Press, 2012)
      Millions of platelets are produced each hour by bone marrow (BM) megakaryocytes (MKs). MKs extend transendothelial proplatelet (PP) extensions into BM sinusoids and shed new platelets into the blood. The mechanisms that ...
    • Pivotal role for skin transendothelial radio-resistant anti-inflammatory macrophages in tissue repair 

      Barreiro, Olga; Cibrian, Danay; Clemente, Cristina; Alvarez, David; Moreno, Vanessa; Valiente, Íñigo; Bernad, Antonio; Vestweber, Dietmar; Arroyo, Alicia G; Martín, Pilar; von Andrian, Ulrich H; Sánchez Madrid, Francisco (eLife Sciences Publications, Ltd, 2016)
      Heterogeneity and functional specialization among skin-resident macrophages are incompletely understood. In this study, we describe a novel subset of murine dermal perivascular macrophages that extend protrusions across ...
    • Prolonged contact with dendritic cells turns lymph node‐resident NK cells into anti‐tumor effectors 

      Mingozzi, Francesca; Spreafico, Roberto; Gorletta, Tatiana; Cigni, Clara; Di Gioia, Marco; Caccia, Michele; Sironi, Laura; Collini, Maddalena; Soncini, Matias; Rusconi, Michela; von Andrian, Ulrich H; Chirico, Giuseppe; Zanoni, Ivan; Granucci, Francesca (John Wiley and Sons Inc., 2016)
      Abstract Natural killer (NK) cells are critical players against tumors. The outcome of anti‐tumor vaccination protocols depends on the efficiency of NK‐cell activation, and efforts are constantly made to manipulate them ...
    • Random Migration and Signal Integration Promote Rapid and Robust T Cell Recruitment 

      Textor, Johannes; Henrickson, Sarah E.; Mandl, Judith N.; von Andrian, Ulrich H.; Westermann, Jürgen; de Boer, Rob J.; Beltman, Joost B. (Public Library of Science, 2014)
      To fight infections, rare T cells must quickly home to appropriate lymph nodes (LNs), and reliably localize the antigen (Ag) within them. The first challenge calls for rapid trafficking between LNs, whereas the second may ...
    • Regulatory iNKT cells lack PLZF expression and control Treg cell and macrophage homeostasis in adipose tissue 

      Lynch, Lydia; Michelet, Xavier; Zhang, Sai; Brennan, Patrick J.; Moseman, Ashley; Lester, Chantel; Besra, Gurdyal; Vomhof-Dekrey, Emilie E.; Tighe, Mike; Koay, Hui-Fern; Godfrey, Dale I.; Leadbetter, Elizabeth A.; Sant’Angelo, Derek B.; von Andrian, Ulrich; Brenner, Michael B. (2015)
      iNKT cells are CD1d-restricted lipid-sensing innate T cells that express the transcription factor PLZF. iNKT cells accumulate in adipose tissue, where they are anti-inflammatory, but the factors that contribute to their ...