Browsing by Author "Lieberman, Judy"
Now showing items 1-9 of 9
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CD4 Aptamer-SiRNA Chimeras (CD4-AsiCs) Knockdown Gene Expression in CD4+ Cells and Inhibit HIV Transmission
Wheeler, Lee Adam (2013-02-08)The continued spread of HIV underscores the need to interrupt transmission. One attractive strategy is a topical microbicide. Sexual transmission of herpes simplex virus type 2 (HSV-2) in mice can be inhibited by intravaginal ... -
Efficient and specific gene knockdown by small interfering RNAs produced in bacteria
Huang, Linfeng; Jin, Jingmin; Deighan, Padraig; Kiner, Evgeny; McReynolds, Larry; Lieberman, Judy (2013)Synthetic small interfering RNAs (siRNAs) are an indispensable tool to investigate gene function in eukaryotic cells1,2 and may be used for therapeutic purposes to knockdown genes implicated in disease3. Thus far, most ... -
Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells
Sharei, Armon; Trifonova, Radiana; Jhunjhunwala, Siddharth; Hartoularos, George C.; Eyerman, Alexandra T.; Lytton-Jean, Abigail; Angin, Mathieu; Sharma, Siddhartha; Poceviciute, Roberta; Mao, Shirley; Heimann, Megan; Liu, Sophia; Talkar, Tanya; Khan, Omar F.; Addo, Marylyn; von Andrian, Ulrich H.; Anderson, Daniel G.; Langer, Robert; Lieberman, Judy; Jensen, Klavs F. (Public Library of Science, 2015)Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery ... -
Harnessing RNA interference to prevent or treat HSV-2 and HIV
Lieberman, Judy (BioMed Central, 2006) -
miR-34 and p53: New Insights into a Complex Functional Relationship
Navarro, Francisco; Lieberman, Judy (Public Library of Science, 2015)miR-34, a tumor suppressor miRNA family transcriptionally activated by p53, is considered a critical mediator of p53 function. However, knockout of the mouse miR-34 family has little or no effect on the p53 response. The ... -
Novel Roles for Ribonucleic Acids in Programmed Cell Death
Thomas, Marshall Peter (2014-10-22)Apoptosis is a tightly coordinated program to shut down and dismantle a cell, characterized by mitochondrial outer membrane permeabilization (MOMP), caspase activation to cleave hundreds of proteins, DNA fragmentation, and ... -
The Rab2A GTPase Promotes Breast Cancer Stem Cells and Tumorigenesis via Erk Signaling Activation
Luo, Man-Li; Gong, Chang; Chen, Chun-Hau; Hu, Hai; Huang, Pengyu; Zheng, Min; Yao, Yandan; Wei, Shuo; Wulf, Gerburg; Lieberman, Judy; Zhou, Xiao Zhen; Song, Erwei; Lu, Kun Ping (2015)SUMMARY Proline-directed phosphorylation is regulated by the prolyl isomerase Pin1, which plays a fundamental role in driving breast cancer stem-like cells (BCSCs). Rab2A is a small GTPase critical for vesicle trafficking. ... -
Sequencing of Captive Target Transcripts Identifies the Network of Regulated Genes and Functions of Primate-Specific miR-522
Tan, Shen Mynn; Kirchner, Rory D; Jin, Jingmin; Hofmann, Oliver Marc; McReynolds, Larry; Hide, Winston; Lieberman, Judy (Elsevier BV, 2014)Identifying microRNA (miRNA)-regulated genes is key to understanding miRNA function. However, many miRNA recognition elements (MREs) do not follow canonical “seed” base-pairing rules, making identification of bona fide ... -
Using NMR to Identify Structural Features of Lin28-Regulated miRNAs and mRNAs and as a Tool for Comparing Differences in Cellular Metabolism
O'Day, Elizabeth Mary (2013-09-30)Part 1 of this thesis seeks to identify shared structural features of Lin28-regulated miRNAs and mRNAs. Lin28 is an evolutionarily conserved, RNA binding protein, highly expressed in stem cells and poorly differentiated ...