Now showing items 1-7 of 7

    • Constant rate of p53 tetramerization in response to DNA damage controls the p53 response 

      Gaglia, Giorgio; Lahav, Galit (BlackWell Publishing Ltd, 2014)
      The dynamics of the tumor suppressor protein p53 have been previously investigated in single cells using fluorescently tagged p53. Such approach reports on the total abundance of p53 but does not provide a measure for ...
    • DNA-damage-induced apoptosis in stem and cancer cells 

      Liu, Julia Chang (2014-06-06)
      This work comprises analyses of cell fate decision-making in response to DNA damage. DNA damage is a ubiquitous threat to genomic stability, and depending on the type and extent of the damage, can lead to widespread changes ...
    • Dynamics of p53 tetramers in live single cells 

      Gaglia, Giorgio (2014-06-06)
      Protein homo-oligomerization is the process through which identical peptides bind together to form higher order complexes. Self-interactions in many cases are constitutive and stable, used as building blocks for biological ...
    • Oscillations and Variability in the P53 System 

      Geva-Zatorsky, Naama; Rosenfeld, Nitzan; Itzkovitz, Shalev; Sigal, Alex; Dekel, Erez; Yarnitzky, Talia; Liron, Yuvalal; Polak, Paz; Alon, Uri; Milo, Ron; Lahav, Galit (Nature Publishing Group, 2006)
      Understanding the dynamics and variability of protein circuitry requires accurate measurements in living cells as well as theoretical models. To address this, we employed one of the best-studied protein circuits in human ...
    • The p53 response in single cells is linearly correlated to the number of DNA breaks without a distinct threshold 

      Loewer, Alexander; Karanam, Ketki; Mock, Caroline; Lahav, Galit (BioMed Central, 2013)
      Background: The tumor suppressor protein p53 is activated by cellular stress. DNA double strand breaks (DSBs) induce the activation of the kinase ATM, which stabilizes p53 and activates its transcriptional activity. Single ...
    • Quantitative Analysis of DNA Repair and p53 in Individual Human Cells 

      Verkhedkar, Ketki Dinesh (2013-03-18)
      The goal of my research was to obtain a quantitative understanding of the mechanisms of DNA double-strand break (DSB) repair, and the activation of the tumor suppressor p53 in response to DSBs in human cells. In Chapter ...
    • Single-cell analysis of circadian dynamics in tissue explants 

      Lande-Diner, Laura; Stewart-Ornstein, Jacob; Weitz, Charles J.; Lahav, Galit (The American Society for Cell Biology, 2015)
      Tracking molecular dynamics in single cells in vivo is instrumental to understanding how cells act and interact in tissues. Current tissue imaging approaches focus on short-term observation and typically nonendogenous or ...