Browsing by Author "Drokhlyansky, Eugene"
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The Brain Has an Innate Immune Response That Can Limit Virus Spread
Drokhlyansky, Eugene (2016-05-17)The brain has a tightly regulated environment that protects non-regenerating post-mitotic neurons and limits inflammation, which led to its description as a site of ‘immune privilege’. For example, viral and bacterial ... -
Cell Type-Specific Manipulation with GFP-Dependent Cre Recombinase
Tang, Jonathan C Y; Rudolph, Stephanie; Dhande, Onkar S; Abraira, Victoria E; Choi, Seungwon; Lapan, Sylvain; Drew, Iain R; Drokhlyansky, Eugene; Huberman, Andrew D; Regehr, Wade G; Cepko, Constance L (2016)Summary There are many transgenic GFP reporter lines that allow visualization of specific populations of cells. Using such lines for functional studies requires a method that transforms GFP into a molecule that enables ... -
Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodies
Tang, Jonathan CY; Drokhlyansky, Eugene; Etemad, Behzad; Rudolph, Stephanie; Guo, Binggege; Wang, Sui; Ellis, Emily G; Li, Jonathan Z; Cepko, Constance L (eLife Sciences Publications, Ltd, 2016)The ability to detect and/or manipulate specific cell populations based upon the presence of intracellular protein epitopes would enable many types of studies and applications. Protein binders such as nanobodies (Nbs) can ... -
Preferential Budding of Vesicular Stomatitis Virus from the Basolateral Surface of Polarized Epithelial Cells Is Not Solely Directed by Matrix Protein or Glycoprotein
Drokhlyansky, Eugene; Soh, Timothy K.; Cepko, Constance L. (American Society for Microbiology, 2015)Vesicular stomatitis virus has been shown to bud basolaterally, and the matrix protein, but not glycoprotein, was proposed to mediate this asymmetry. Using polarized T84 monolayers, we demonstrate that no single viral ...