Browsing by Author "Singh, Sasha"

Now showing items 1-4 of 4

    • Endophenotype Network Models: Common Core of Complex Diseases 

      Ghiassian, Susan Dina; Menche, Jörg; Chasman, Daniel I.; Giulianini, Franco; Wang, Ruisheng; Ricchiuto, Piero; Aikawa, Masanori; Iwata, Hiroshi; Müller, Christian; Zeller, Tania; Sharma, Amitabh; Wild, Philipp; Lackner, Karl; Singh, Sasha; Ridker, Paul M.; Blankenberg, Stefan; Barabási, Albert-László; Loscalzo, Joseph (Nature Publishing Group, 2016)
      Historically, human diseases have been differentiated and categorized based on the organ system in which they primarily manifest. Recently, an alternative view is emerging that emphasizes that different diseases often have ...

    • Enrichment of calcifying extracellular vesicles using density-based ultracentrifugation protocol 

      Hutcheson, Joshua D.; Goettsch, Claudia; Pham, Tan; Iwashita, Masaya; Aikawa, Masanori; Singh, Sasha A.; Aikawa, Elena (Co-Action Publishing, 2014)
      Calcifying extracellular vesicles (EVs) released from cells within atherosclerotic plaques have received increased attention for their role in mediating vascular calcification, a major predictor of cardiovascular morbidity ...

    • FLEXIQinase, a mass spectrometry-based assay, to unveil multi-kinase mechanisms 

      Singh, Sasha Anna; Winter, Dominic; Bilimoria, Parizad Maheyar; Bonni, Azad; Steen, Hanno; Steen, Judith A. (2013)
      We introduce a mass spectrometry-based method that provides residue-resolved quantitative information about protein phosphorylation. In this FLEXIQinase assay we combined our Full-Length Expressed Stable Isotope-labeled ...

    • PARP9 and PARP14 cross-regulate macrophage activation via STAT1 ADP-ribosylation 

      Iwata, Hiroshi; Goettsch, Claudia; Sharma, Amitabh; Ricchiuto, Piero; Goh, Wilson Wen Bin; Halu, Arda; Yamada, Iwao; Yoshida, Hideo; Hara, Takuya; Wei, Mei; Inoue, Noriyuki; Fukuda, Daiju; Mojcher, Alexander; Mattson, Peter C.; Barabási, Albert-László; Boothby, Mark; Aikawa, Elena; Singh, Sasha A.; Aikawa, Masanori (Nature Publishing Group, 2016)
      Despite the global impact of macrophage activation in vascular disease, the underlying mechanisms remain obscure. Here we show, with global proteomic analysis of macrophage cell lines treated with either IFNγ or IL-4, that ...