Now showing items 1-20 of 20

    • Ablation of TRIP-Br2, a novel regulator of fat lipolysis, thermogenesis and oxidative metabolism, prevents diet-induced obesity and insulin resistance 

      Liew, Chong Wee; Boucher, Jeremie; Cheong, Jit Kong; Vernochet, Cecile; Koh, Ho-Jin; Mallol, Cristina; Townsend, Kristy; Langin, Dominique; Kawamori, Dan; Hu, Jiang; Tseng, Yu-Hua; Hellerstein, Marc K; Farmer, Stephen R; Goodyear, Laurie; Doria, Alessandro; Blüher, Matthias; Hsu, Stephen I-Hong; Kulkarni, Rohit N (2012)
      SUMMARY Obesity develops due to altered energy homeostasis favoring fat storage. Here we describe a novel transcription co-regulator for adiposity and energy metabolism, TRIP-Br2 (also called SERTAD2). TRIP-Br2 null mice ...
    • Cannabinoids Inhibit Insulin Receptor Signaling in Pancreatic \(\beta\)-Cells 

      Doyle, Máire E.; Liu, Zhuo; Lao, Qizong; Shin, Yu-Kyong; Carlson, Olga D.; Thomas, Sam; Napora, Joshua K.; Moaddel, Ruin; Maudsley, Stuart; Martin, Bronwen; Egan, Josephine M.; Kim, Wookhyun; Kim, Hee Seung; Lee, Eun-Kyung; Wang, Yan; Kulkarni, Rohit Narayan (American Diabetes Association, 2011)
      Objective: Optimal glucose homeostasis requires exquisitely precise adaptation of the number of insulin-secreting \(\beta\)-cells in the islets of Langerhans. Insulin itself positively regulates \(\beta\)-cell proliferation ...
    • Cyclin D2 is Essential for the Compensatory Beta-Cell Hyperplastic Response to Insulin Resistance in Rodents 

      Georgia, Senta; Hinault, Charlotte; Kawamori, Dan; Hu, Jiang; Meyer, John; Kanji, Murtaza; Bhushan, Anil; Kulkarni, Rohit Narayan (American Diabetes Association, 2010)
      OBJECTIVE: A major determinant of the progression from insulin resistance to the development of overt type 2 diabetes is a failure to mount an appropriate compensatory β-cell hyperplastic response to maintain normoglycemia. ...
    • \(\Delta\)40 Isoform of p53 Controls \(\beta\)-Cell Proliferation and Glucose Homeostasis in Mice 

      Hinault, Charlotte; Kawamori, Dan; Liew, Chong-Wee; Maier, Bernhard; Hu, Jiang; Keller, Susanna R.; Mirmira, Raghavendra G.; Scrable, Heidi; Kulkarni, Rohit Narayan (American Diabetes Association, 2011)
      Objective: Investigating the dynamics of pancreatic \(\beta\)-cell mass is critical for developing strategies to treat both type 1 and type 2 diabetes. p53, a key regulator of the cell cycle and apoptosis, has mostly been ...
    • Dissecting diabetes/metabolic disease mechanisms using pluripotent stem cells and genome editing tools 

      Teo, Adrian Kee Keong; Gupta, Manoj K.; Doria, Alessandro; Kulkarni, Rohit N. (Elsevier, 2015)
      Background: Diabetes and metabolic syndromes are chronic, devastating diseases with increasing prevalence. Human pluripotent stem cells are gaining popularity in their usage for human in vitro disease modeling. With recent ...
    • Forced Hepatic Overexpression of CEACAM1 Curtails Diet-Induced Insulin Resistance 

      Al-Share, Qusai Y.; DeAngelis, Anthony M.; Lester, Sumona Ghosh; Bowman, Thomas A.; Ramakrishnan, Sadeesh K.; Abdallah, Simon L.; Russo, Lucia; Patel, Payal R.; Kaw, Meenakshi K.; Raphael, Christian K.; Kim, Andrea Jung; Heinrich, Garrett; Lee, Abraham D.; Kim, Jason K.; Kulkarni, Rohit N.; Philbrick, William M.; Najjar, Sonia M. (American Diabetes Association, 2015)
      Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) regulates insulin sensitivity by promoting hepatic insulin clearance. Liver-specific inactivation or global null-mutation of Ceacam1 impairs hepatic insulin ...
    • Gene Silencing of Phogrin Unveils Its Essential Role in Glucose-Responsive Pancreatic β-Cell Growth 

      Torii, Seiji; Saito, Naoya; Kawano, Ayumi; Hou, Ni; Ueki, Kohjiro; Kulkarni, Rohit Narayan; Takeuchi, Toshiyuki (American Diabetes Association, 2009)
      OBJECTIVE—Phogrin and IA-2, autoantigens in insulin-dependent diabetes, have been shown to be involved in insulin secretion in pancreatic β-cells; however, implications at a molecular level are confusing from experiment ...
    • Human β-Cell Proliferation and Intracellular Signaling Part 2: Still Driving in the Dark Without a Road Map 

      Bernal-Mizrachi, Ernesto; Kulkarni, Rohit N.; Scott, Donald K.; Mauvais-Jarvis, Franck; Stewart, Andrew F.; Garcia-Ocaña, Adolfo (American Diabetes Association, 2014)
      Enhancing β-cell proliferation is a major goal for type 1 and type 2 diabetes research. Unraveling the network of β-cell intracellular signaling pathways that promote β-cell replication can provide the tools to address ...
    • Human β-Cell Proliferation and Intracellular Signaling: Driving in the Dark Without a Road Map 

      Kulkarni, Rohit N.; Mizrachi, Ernesto-Bernal; Ocana, Adolfo Garcia; Stewart, Andrew F. (American Diabetes Association, 2012)
      A major goal in diabetes research is to find ways to enhance the mass and function of insulin secreting β-cells in the endocrine pancreas to prevent and/or delay the onset or even reverse overt diabetes. In this Perspectives ...
    • Insufficiency of Janus Kinase 2-Autonomous Leptin Receptor Signals for Most Physiologic Leptin Actions 

      Robertson, Scott; Ishida-Takahashi, Ryoko; Tawara, Isao; Hu, Jiang; Patterson, Christa M.; Jones, Justin C.; Kulkarni, Rohit Narayan; Myers, Martin G. (American Diabetes Association, 2010)
      OBJECTIVE: Leptin acts via its receptor (LepRb) to signal the status of body energy stores. Leptin binding to LepRb initiates signaling by activating the associated Janus kinase 2 (Jak2) tyrosine kinase, which promotes the ...
    • Insulin Augmentation of Glucose-Stimulated Insulin Secretion Is Impaired in Insulin-Resistant Humans 

      Halperin, Florencia; Lopez, Ximena; Manning, Raquel; Kahn, C. Ronald; Kulkarni, Rohit Narayan; Goldfine, Allison Braunwald (American Diabetes Association, 2012)
      Type 2 diabetes (T2D) is characterized by insulin resistance and pancreatic β-cell dysfunction, the latter possibly caused by a defect in insulin signaling in β-cells. We hypothesized that insulin’s effect to potentiate ...
    • Insulin Resistance Alters Islet Morphology in Nondiabetic Humans 

      Mezza, Teresa; Muscogiuri, Giovanna; Sorice, Gian Pio; Clemente, Gennaro; Hu, Jiang; Pontecorvi, Alfredo; Holst, Jens J.; Giaccari, Andrea; Kulkarni, Rohit N. (American Diabetes Association, 2014)
      Type 2 diabetes is characterized by poor glucose uptake in metabolic tissues and manifests when insulin secretion fails to cope with worsening insulin resistance. In addition to its effects on skeletal muscle, liver, and ...
    • Insulin Signaling Regulates Mitochondrial Function in Pancreatic β-Cells 

      Liu, Siming; Okada, Terumasa; Assmann, Anke; Soto, Jamie; Liew, Chong-Wee; Bugger, Heiko; Shirihai, Orian S.; Abel, E. Dale; Kulkarni, Rohit Narayan (Public Library of Science, 2009)
      Insulin/IGF-I signaling regulates the metabolism of most mammalian tissues including pancreatic islets. To dissect the mechanisms linking insulin signaling with mitochondrial function, we first identified a mitochondria-tethering ...
    • IRS1 deficiency protects β-cells against ER stress-induced apoptosis by modulating sXBP-1 stability and protein translation 

      Takatani, Tomozumi; Shirakawa, Jun; Roe, Michael W.; Leech, Colin A.; Maier, Bernhard F.; Mirmira, Raghavendra G.; Kulkarni, Rohit N. (Nature Publishing Group, 2016)
      Endoplasmic reticulum (ER) stress is among several pathological features that underlie β-cell failure in the development of type 1 and type 2 diabetes. Adaptor proteins in the insulin/insulin-like-growth factor-1 signaling ...
    • The Islet Estrogen Receptor-α Is Induced by Hyperglycemia and Protects Against Oxidative Stress-Induced Insulin-Deficient Diabetes 

      Kilic, Gamze; Alvarez-Mercado, Ana I.; Zarrouki, Bader; Opland, Darren; Liew, Chong Wee; Alonso, Laura C.; Myers, Martin G.; Jonas, Jean-Christophe; Poitout, Vincent; Kulkarni, Rohit N.; Mauvais-Jarvis, Franck (Public Library of Science, 2014)
      The female steroid, 17β-estradiol (E2), is important for pancreatic β-cell function and acts via at least three estrogen receptors (ER), ERα, ERβ, and the G-protein coupled ER (GPER). Using a pancreas-specific ERα knockout ...
    • The Polycomb protein, Bmi1, regulates insulin sensitivity 

      Cannon, Corey E.; Titchenell, Paul M.; Groff, David N.; El Ouaamari, Abdelfattah; Kulkarni, Rohit N.; Birnbaum, Morris J.; Stoffers, Doris A. (Elsevier, 2014)
      Objective: The Polycomb Repressive Complexes (PRC) 1 and 2 function to epigenetically repress target genes. The PRC1 component, Bmi1, plays a crucial role in maintenance of glucose homeostasis and beta cell mass through ...
    • Regulation of Calcium-Permeable TRPV2 Channel by Insulin in Pancreatic β-Cells 

      Hisanaga, Etsuko; Nagasawa, Masahiro; Ueki, Kohjiro; Kulkarni, Rohit Narayan; Mori, Masatomo; Kojima, Itaru (American Diabetes Association, 2009)
      OBJECTIVE—Calcium-permeable cation channel TRPV2 is expressed in pancreatic β-cells. We investigated regulation and function of TRPV2 in β-cells. RESEARCH DESIGN AND METHODS—Translocation of TRPV2 was assessed in MIN6 cells ...
    • The Role of Proinflammatory Cytokines on Pancreatic Cell Plasticity 

      Valdez, Ivan A. (2016-05-05)
      Diabetes mellitus is a complex and heterogeneous group of metabolic diseases characterized by a disruption in blood glucose homeostasis. This could result from an autoimmune destruction of insulin-producing pancreatic beta ...
    • Tribbles 3 Mediates Endoplasmic Reticulum Stress-Induced Insulin Resistance in Skeletal Muscle 

      Koh, Ho-Jin; Toyoda, Taro; Didesch, Michelle M.; Lee, Min-Young; Sleeman, Mark W.; Kulkarni, Rohit N.; Musi, Nicolas; Hirshman, Michael F.; Goodyear, Laurie J. (2013)
      Endoplasmic Reticulum (ER) stress has been linked to insulin resistance in multiple tissues but the role of ER stress in skeletal muscle has not been explored. ER stress has also been reported to increase tribbles 3 (TRB3) ...
    • X-Box Binding Protein 1 Is Essential for Insulin Regulation of Pancreatic α-Cell Function 

      Akiyama, Masaru; Liew, Chong Wee; Lu, Shusheng; Hu, Jiang; Martinez, Rachael; Hambro, Ben; Kennedy, Robert T.; Kulkarni, Rohit N. (American Diabetes Association, 2013)
      Patients with type 2 diabetes (T2D) often exhibit hyperglucagonemia despite hyperglycemia, implicating defective α-cell function. Although endoplasmic reticulum (ER) stress has been suggested to underlie β-cell dysfunction ...