Browsing by Author "Villalta, Christians"

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    • Exploiting Position Effects and the Gypsy Retrovirus Insulator to Engineer Precisely Expressed Transgenes 

      Markstein, Michele; Pitsouli, Chrysoula; Villalta, Christians; Celniker, Susan E.; Perrimon, Norbert (Springer Science and Business Media LLC, 2008-03-02)
      A major obstacle to creating precisely expressed transgenes lies in the epigenetic effects of the host chromatin that surrounds them. Here we present a strategy to overcome this problem, employing a Gal4-inducible luciferase ...

    • Hierarchical Rules for Argonaute Loading in Drosophila 

      Czech, Benjamin; Zhou, Rui; Erlich, Yaniv; Brennecke, Julius; Binari, Richard; Villalta, Christians; Gordon, Assaf; Perrimon, Norbert; Hannon, Gregory J. (Elsevier BV, 2009-11)
      Drosophila Argonaute-1 and Argonaute-2 differ in function and small RNA content. AGO2 binds to siRNAs, whereas AGO1 is almost exclusively occupied by microRNAs. MicroRNA duplexes are intrinsically asymmetric, with one ...

    • An Integrative Analysis of the InR/PI3K/Akt Network Identifies the Dynamic Response to Insulin Signaling 

      Vinayagam, Arunachalam; Kulkarni, Meghana; Sopko, Richelle; Sun, Xiaoyun; Hu, Yanhui; Nand, Ankita; Villalta, Christians; Moghimi, Ahmadali; Yang, Xuemei; Mohr, Stephanie; Hong, Pengyu; Asara, John; Perrimon, Norbert (Elsevier BV, 2016-09-13)
      Insulin regulates an essential conserved signaling pathway affecting growth, proliferation and metabolism. To expand our understanding of the insulin pathway, we combine biochemical, genetic and computational approaches ...

    • The Twin Spot Generator for Differential Drosophila Lineage Analysis 

      Griffin, Ruth; Sustar, Anne; Bonvin, Marianne; Binari, Richard; del Valle Rodriguez, Alberto; Hohl, Amber M; Bateman, Jack R; Villalta, Christians; Heffern, Elleard; Grunwald, Didier; Bakal, Chris; Desplan, Claude; Schubiger, Gerold; Wu, C-ting; Perrimon, Norbert (Springer Science and Business Media LLC, 2009-07-26)
      In Drosophila, widely-used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the Twin ...

    • Vector and Parameters for Targeted Transgenic RNA Interference in Drosophila melanogaster 

      Ni, Jian-Quan; Markstein, Michele; Binari, Richard; Pfeiffer, Barret D.; Liu, Lu-Ping; Villalta, Christians; Booker, Matthew; Perkins, Lizabeth; Perrimon, Norbert (Springer Nature, 2008-01)
      The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site–driven RNA interference constructs ...