Now showing items 1-4 of 4

    • DNA methylation dynamics of the human preimplantation embryo 

      Smith, Zachary D.; Chan, Michelle M.; Humm, Kathryn C.; Karnik, Rahul; Mekhoubad, Shila; Regev, Aviv; Eggan, Kevin; Meissner, Alexander (2014)
      In mammals, cytosine methylation is predominantly restricted to CpG dinucleotides and stably distributed across the genome, with local, cell type-specific regulation directed by DNA binding factors1-3. This comparatively ...
    • Proto-Oncogenic Role of Mutant IDH2 in Leukemia Initiation and Maintenance 

      Kats, Lev M.; Reschke, Markus; Taulli, Riccardo; Pozdnyakova, Olga O.; Burgess, Kerri; Bhargava, Parul; Straley, Kimberly; Karnik, Rahul; Meissner, Alexander; Small, Donald; Su, Shinsan M.; Yen, Katharine; Zhang, Jiangwen; Pandolfi, Pier Paolo (Elsevier BV, 2014)
      Mutations in the metabolic enzymes isocitrate dehydrogenase-1 (IDH1) and IDH2 that produce the oncometabolite D-2-hydroxyglutarate (2-HG) occur frequently in human acute myeloid leukemia (AML). 2-HG modulates numerous ...
    • Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells 

      Liao, Jing; Karnik, Rahul; Gu, Hongcang; Ziller, Michael J.; Clement, Kendell; Tsankov, Alexander M.; Akopian, Veronika; Gifford, Casey A.; Donaghey, Julie; Galonska, Christina; Pop, Ramona; Reyon, Deepak; Tsai, Shengdar Q.; Mallard, William; Joung, J. Keith; Rinn, John L.; Gnirke, Andreas; Meissner, Alexander (2015)
      DNA methylation is a key epigenetic modification involved in regulating gene expression and maintaining genomic integrity. Here we inactivated all three catalytically active DNA methyltransferases in human embryonic stem ...
    • X Chromosome Reactivation Dynamics Reveal Stages of Reprogramming to Pluripotency 

      Pasque, Vincent; Tchieu, Jason; Karnik, Rahul; Uyeda, Molly; Sadhu Dimashkie, Anupama; Case, Dana; Papp, Bernadett; Bonora, Giancarlo; Patel, Sanjeet; Ho, Ritchie; Schmidt, Ryan; McKee, Robin; Sado, Takashi; Tada, Takashi; Meissner, Alexander; Plath, Kathrin (Elsevier BV, 2014)
      Reprogramming to iPSCs resets the epigenome of somatic cells, including the reversal of X chromosome inactivation. We sought to gain insight into the steps underlying the reprogramming process by examining the means by ...