Browsing by Author "Ausubel, Frederick"
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Antifungal Chemical Compounds Identified Using a C. elegans Pathogenicity Assay
Breger, Julia; Fuchs, Beth Burgwyn; Aperis, George; Moy, Terence I; Ausubel, Frederick M.; Mylonakis, Eleftherios; Cormack, Brendan P (Public Library of Science, 2007)There is an urgent need for the development of new antifungal agents. A facile in vivo model that evaluates libraries of chemical compounds could solve some of the main obstacles in current antifungal discovery. We show ... -
Characterization of the integrated filamentous phage Pf5 and its involvement in small-colony formation
Mooij, Marlies J.; Drenkard, Eliana; Llamas, María A.; Vandenbroucke-Grauls, Christina M. J. E.; Savelkoul, Paul H. M.; Ausubel, Frederick M.; Bitter, Wilbert (Society for General Microbiology, 2007)Bacteriophages play an important role in bacterial virulence and phenotypic variation. It has been shown that filamentous bacteriophage Pf4 of Pseudomonas aeruginosa strain PAO1 mediates the formation of small-colony ... -
Contribution of Gelatinase, Serine Protease, and fsr to the Pathogenesis of Enterococcus faecalis Endophthalmitis
Engelbert, M.; Mylonakis, E; Ausubel, Frederick M.; Calderwood, Stephen Beaven; Gilmore, Michael S. (American Society for Microbiology, 2004)Gelatinase and serine protease were found to contribute in concert to pathogenesis in a rabbit model of endophthalmitis. However, a mutant defective in the fsr regulator was observed to be more attenuated than a mutant ... -
A Defensin from the Model Beetle Tribolium castaneum Acts Synergistically with Telavancin and Daptomycin against Multidrug Resistant Staphylococcus aureus
Rajamuthiah, Rajmohan; Jayamani, Elamparithi; Conery, Annie L.; Fuchs, Beth Burgwyn; Kim, Wooseong; Johnston, Tatiana; Vilcinskas, Andreas; Ausubel, Frederick M.; Mylonakis, Eleftherios (Public Library of Science, 2015)The red flour beetle Tribolium castaneum is a common insect pest and has been established as a model beetle to study insect development and immunity. This study demonstrates that defensin 1 from T. castaneum displays in ... -
The Enterococcus faecalis fsrB Gene, a Key Component of the fsr Quorum-Sensing System, Is Associated with Virulence in the Rabbit Endophthalmitis Model
Mylonakis, E.; Engelbert, M.; Qin, X.; Sifri, C. D.; Murray, B. E.; Ausubel, Frederick M.; Gilmore, Michael S.; Calderwood, Stephen Beaven (American Society for Microbiology, 2002)We used a rabbit endophthalmitis model to explore the role of fsrB, a gene required for the function of the fsr quorum-sensing system of Enterococcus faecalis, in pathogenicity. A nonpolar deletion mutant of fsrB had ... -
The Evolutionarily Conserved Mediator Subunit MDT-15/MED15 Links Protective Innate Immune Responses and Xenobiotic Detoxification
Pukkila-Worley, Read; Feinbaum, Rhonda L.; McEwan, Deborah L.; Conery, Annie L.; Ausubel, Frederick M. (Public Library of Science, 2014)Metazoans protect themselves from environmental toxins and virulent pathogens through detoxification and immune responses. We previously identified a small molecule xenobiotic toxin that extends survival of Caenorhabditis ... -
Genes Involved in the Evolution of Herbivory by a Leaf-Mining, Drosophilid Fly
Whiteman, Noah K.; Gloss, Andrew D.; Sackton, Timothy; Groen, Simon C.; Humphrey, Parris T.; Lapoint, Richard T.; Sønderby, Ida E.; Halkier, Barbara A.; Kocks, Christine; Ausubel, Frederick M.; Pierce, Naomi Ellen (Oxford University Press (OUP), 2012)Herbivorous insects are among the most successful radiations of life. However, we know little about the processes underpinning the evolution of herbivory. We examined the evolution of herbivory in the fly, Scaptomyza flava, ... -
Genome-Wide Identification of Pseudomonas aeruginosa Virulence-Related Genes Using a Caenorhabditis elegans Infection Model
Feinbaum, Rhonda Lorin; Urbach, Jonathan M.; Liberati, Nicole T.; Djonovic, Slavica; Adonizio, Allison; Carvunis, Anne-Ruxandra; Ausubel, Frederick M. (Public Library of Science, 2012)Pseudomonas aeruginosa strain PA14 is an opportunistic human pathogen capable of infecting a wide range of organisms including the nematode Caenorhabditis elegans. We used a non-redundant transposon mutant library consisting ... -
Identification of an Antimicrobial Agent Effective against Methicillin-Resistant Staphylococcus aureus Persisters Using a Fluorescence-Based Screening Strategy
Kim, Wooseong; Conery, Annie L.; Rajamuthiah, Rajmohan; Fuchs, Beth Burgwyn; Ausubel, Frederick M.; Mylonakis, Eleftherios (Public Library of Science, 2015)Persisters are a subpopulation of normal bacterial cells that show tolerance to conventional antibiotics. Persister cells are responsible for recalcitrant chronic infections and new antibiotics effective against persisters ... -
Identification of Antifungal Compounds Active against Candida albicans Using an Improved High-Throughput Caenorhabditis elegans Assay
Okoli, Ikechukwu; Tempakakis, Emmanouil; Holson, Edward; Wagner, Florence; Conery, Annie L.; Larkins-Ford, Jonah; Stern, Andy; Coleman, Jeffrey James; An, W. Frank; Wu, Gang; Ausubel, Frederick M.; Mylonakis, Eleftherios (Public Library of Science, 2009)Candida albicans, the most common human pathogenic fungus, can establish a persistent lethal infection in the intestine of the microscopic nematode Caenorhabditis elegans. The C. elegans–C. albicans infection model was ... -
Identification of Pseudomonas aeruginosa Phenazines that Kill Caenorhabditis elegans
Cezairliyan, Brent Orvin; Vinayavekhin, Nawaporn; Grenfell-Lee, Daniel; Yuen, Grace J.; Saghatelian, Alan; Ausubel, Frederick M. (Public Library of Science, 2013)Pathogenic microbes employ a variety of methods to overcome host defenses, including the production and dispersal of molecules that are toxic to their hosts. Pseudomonas aeruginosa, a Gram-negative bacterium, is a pathogen ... -
Influence of maternal breast milk ingestion on acquisition of the intestinal microbiome in preterm infants
Gregory, Katherine E.; Samuel, Buck S.; Houghteling, Pearl; Shan, Guru; Ausubel, Frederick M.; Sadreyev, Ruslan I.; Walker, W. Allan (BioMed Central, 2016)Background: The initial acquisition and early development of the intestinal microbiome during infancy are important to human health across the lifespan. Mode of birth, antibiotic administration, environment of care, and ... -
Microsporidia are Natural Intracellular Parasites of the Nematode Caenorhabditis Elegans
Troemel, Emily R; Félix, Marie-Anne; Whiteman, Noah K.; Barrière, Antoine; Ausubel, Frederick (Public Library of Science, 2008)For decades the soil nematode Caenorhabditis elegans has been an important model system for biology, but little is known about its natural ecology. Recently, C. elegans has become the focus of studies of innate immunity ... -
Mining the Plant-Herbivore Interface with a Leafmining Drosophila of Arabidopsis
Whiteman, Noah K.; Groen, Simon C.; Chevasco, Daniela; Bear, Ashley; Beckwith, Noor M R; Gregory, T. Ryan; Denoux, Carine; Mammarella, Nicole; Ausubel, Frederick M.; Pierce, Naomi E. (Wiley-Blackwell, 2010)Experimental infections of Arabidopsis thaliana (Arabidopsis) with genomically characterized plant pathogens such as Pseudomonas syringae have facilitated the dissection of canonical eukaryotic defence pathways and parasite ... -
p38 MAPK Regulates Expression of Immune Response Genes and Contributes to Longevity in C. elegans
Troemel, Emily R; Chu, Stephanie W; Reinke, Valerie; Lee, Siu Sylvia; Ausubel, Frederick M.; Kim, Dennis H (Public Library of Science, 2006)The PMK-1 p38 mitogen-activated protein kinase pathway and the DAF-2–DAF-16 insulin signaling pathway control Caenorhabditis elegans intestinal innate immunity. pmk-1 loss-of-function mutants have enhanced sensitivity to ... -
Pathogen-Secreted Proteases Activate a Novel Plant Immune Pathway
Cheng, Zhenyu; Li, Jian-Feng; Niu, Yajie; Zhang, Xue-Cheng; Woody, Owen Z.; Xiong, Yan; Djonović, Slavica; Millet, Yves; Bush, Jenifer; McConkey, Brendan J.; Sheen, Jen; Ausubel, Frederick M. (2015)Mitogen-Activated Protein Kinase (MAPK) cascades play central roles in innate immune signaling networks in plants and animals1,2. In plants, however, the molecular mechanisms of how signal perception is transduced to MAPK ... -
Repurposing Salicylanilide Anthelmintic Drugs to Combat Drug Resistant Staphylococcus aureus
Rajamuthiah, Rajmohan; Fuchs, Beth Burgwyn; Conery, Annie L.; Kim, Wooseong; Jayamani, Elamparithi; Kwon, Bumsup; Ausubel, Frederick M.; Mylonakis, Eleftherios (Public Library of Science, 2015)Staphylococcus aureus is a Gram-positive bacterium that has become the leading cause of hospital acquired infections in the US. Repurposing Food and Drug Administration (FDA) approved drugs for antimicrobial therapy involves ... -
Stimulation of Host Immune Defenses by a Small Molecule Protects C. elegans from Bacterial Infection
Pukkila-Worley, Read; Feinbaum, Rhonda Lorin; Kirienko, Natalia; Larkins-Ford, Jonah; Conery, Annie L.; Ausubel, Frederick M. (Public Library of Science, 2012)The nematode Caenorhabditis elegans offers currently untapped potential for carrying out high-throughput, live-animal screens of low molecular weight compound libraries to identify molecules that target a variety of cellular ... -
Trehalose Biosynthesis Promotes Pseudomonas aeruginosa Pathogenicity in Plants
Djonović, Slavica; Urbach, Jonathan M.; Drenkard, Eliana Patricia; Bush, Jenifer; Feinbaum, Rhonda Lorin; Ausubel, Jonathan Lee; Traficante, David; Risech, Martina; Kocks, Christine; Fischbach, Michael A.; Priebe, Gregory Paul; Ausubel, Frederick M. (Public Library of Science, 2013)Pseudomonas aeruginosa strain PA14 is a multi-host pathogen that infects plants, nematodes, insects, and vertebrates. Many PA14 factors are required for virulence in more than one of these hosts. Noting that plants have a ... -
Whole Animal Automated Platform for Drug Discovery against Multi-Drug Resistant Staphylococcus aureus
Rajamuthiah, Rajmohan; Fuchs, Beth Burgwyn; Jayamani, Elamparithi; Kim, Younghoon; Larkins-Ford, Jonah; Conery, Annie; Ausubel, Frederick M.; Mylonakis, Eleftherios (Public Library of Science, 2014)Staphylococcus aureus, the leading cause of hospital-acquired infections in the United States, is also pathogenic to the model nematode Caenorhabditis elegans. The C. elegans-S. aureus infection model was previously carried ...