Browsing by Author "Inoue, Azusa"
Now showing items 1-11 of 11
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5mC and 5hmC dynamics during PGC reprogramming and role of Tet1 in female meiosis
Yamaguchi, Shinpei; Hong, Kwonho; Liu, Rui; Shen, Li; Inoue, Azusa; Diep, Dinh; Zhang, Kun; Zhang, Yi (BioMed Central, 2013) -
Dynamics of 5-methylcytosine and 5-hydroxymethylcytosine during germ cell reprogramming
Yamaguchi, Shinpei; Hong, Kwonho; Liu, Rui; Inoue, Azusa; Shen, Li; Zhang, Kun; Zhang, Yi (Nature Publishing Group, 2013)Previous studies have revealed that mouse primordial germ cells (PGCs) undergo genome-wide DNA methylation reprogramming to reset the epigenome for totipotency. However, the precise 5-methylcytosine (5mC) dynamics and its ... -
Embryonic Development following Somatic Cell Nuclear Transfer Impeded by Persisting Histone Methylation
Matoba, Shogo; Liu, Yuting; Lu, Falong; Iwabuchi, Kumiko A; Inoue, Azusa; Zhang, Yi (Elsevier BV, 2014)Mammalian oocytes can reprogram somatic cells into a totipotent state enabling animal cloning through somatic cell nuclear transfer (SCNT). However, the majority of SCNT embryos fail to develop to term due to undefined ... -
Establishing Chromatin Regulatory Landscape during Mouse Preimplantation Development
Lu, Falong; Liu, Yuting; Inoue, Azusa; Suzuki, Tsukasa; Zhao, Keji; Zhang, Yi (Elsevier BV, 2016)How the chromatin regulatory landscape in the inner cell mass cells is established from differentially packaged sperm and egg genomes during preimplantation development is unknown. Here, we develop a low-input DNase I ... -
Haploinsufficiency, but Not Defective Paternal 5mC Oxidation, Accounts for the Developmental Defects of Maternal Tet3 Knockouts
Inoue, Azusa; Shen, Li; Matoba, Shogo; Zhang, Yi (Elsevier BV, 2015)Paternal DNA demethylation in mammalian zygotes is achieved through Tet3-mediated iterative oxidation of 5-methylcytosine (5mC) coupled with replication-dependent dilution. Tet3-mediated paternal DNA demethylation is ... -
HIRA-mediated H3.3 deposition is required for de novo paternal nuclear envelope formation in mouse zygotes
Inoue, Azusa; Zhang, Yi (BioMed Central, 2013) -
Nucleosome Assembly Is Required for Nuclear Pore Complex Assembly in Mouse Zygotes
Inoue, Azusa; Zhang, Yi (Springer Nature, 2014)Packaging of DNA into nucleosomes not only helps to store genetic information but also creates diverse means for regulating DNA-templated processes. Attempts to reveal additional functions of the nucleosome have been ... -
Simultaneous mapping of active DNA demethylation and sister chromatid exchange in single cells
Wu, Xiaoji; Inoue, Azusa; Suzuki, Tsukasa; Zhang, Yi (Cold Spring Harbor Laboratory Press, 2017)To understand mammalian active DNA demethylation, various methods have been developed to map the genomic distribution of the demethylation intermediates 5-formylcysotine (5fC) and 5-carboxylcytosine (5caC). However, the ... -
siRNA-mediated depletion of stable proteins in mouse oocytes
Inoue, Azusa; Sunaga, Kazuko; Aoki, Fugaku; Zhang, Yi (Nature Publishing Group, 2014)Maternal proteins stored in mammalian oocytes play various roles in meiotic maturation, fertilization, and preimplantation development. To understand biological functions and molecular mechanisms underlying the dynamic ... -
Tet1 controls meiosis by regulating meiotic gene expression
Yamaguchi, Shinpei; Hong, Kwonho; Liu, Rui; Shen, Li; Inoue, Azusa; Diep, Dinh; Zhang, Kun; Zhang, Yi (2012)Meiosis is a germ cell-specific cell division process through which haploid gametes are produced for sexual reproduction1. Prior to initiation of meiosis, mouse primordial germ cells (PGCs) undergo a series of epigenetic ... -
Tet3 and DNA Replication Mediate Demethylation of Both the Maternal and Paternal Genomes in Mouse Zygotes
Shen, Li; Inoue, Azusa; He, Jin; Liu, Yuting; Lu, Falong; Zhang, Yi (Elsevier BV, 2014)With the exception of imprinted genes and certain repeats, DNA methylation is globally erased during preimplantation development. Recent studies have suggested that Tet3-mediated oxidation of 5-methylcytosine (5mC) and DNA ...