Now showing items 1-14 of 14

    • Ascorbic Acid Prevents Loss of Dlk1-Dio3 Imprinting and Facilitates Generation of All-iPS Cell Mice from Terminally Differentiated B Cells 

      Stadtfeld, Matthias; Apostolou, Effie; Chen, Taiping; Oi, Steen; Bestor, Tim; Ferrari, Francesco; Choi, Jiho; Walsh, Ryan Michael; Kim, Sang Yong; Shioda, Toshi; Park, Peter J.; Hochedlinger, Konrad (Nature Publishing Group, 2012)
      The generation of induced pluripotent stem cells (iPSCs) often results in aberrant epigenetic silencing of the imprinted Dlk1-Dio3 gene cluster, compromising the ability to generate entirely iPSC-derived adult mice ('all-iPSC ...
    • Cell Type of Origin Influences the Molecular and Functional Properties of Mouse Induced Pluripotent Stem Cells 

      Polo, Jose M.; Liu, Susanna; Figueroa, Maria Eugenia; Kulalert, Warakorn; Eminli, Sarah; Tan, Kah Yong; Apostolou, Effie; Stadtfeld, Matthias; Li, Yushan; Shioda, Toshihiro; Natesan, Sridaran; Wagers, Amy Jo; Melnick, Ari; Evans, Todd; Hochedlinger, Konrad (Nature Publishing Group, 2010)
      Induced pluripotent stem cells (iPSCs) have been derived from various somatic cell populations through ectopic expression of defined factors. It remains unclear whether iPSCs generated from different cell types are molecularly ...
    • Distinct, strict requirements for Gfi-1b in adult bone marrow red cell and platelet generation 

      Foudi, Adlen; Kramer, Daniel J.; Qin, Jinzhong; Ye, Denise; Behlich, Anna-Sophie; Mordecai, Scott; Preffer, Frederic I.; Amzallag, Arnaud; Ramaswamy, Sridhar; Hochedlinger, Konrad; Orkin, Stuart H.; Hock, Hanno (The Rockefeller University Press, 2014)
      The zinc finger transcriptional repressor Gfi-1b is essential for erythroid and megakaryocytic development in the embryo. Its roles in the maintenance of bone marrow erythropoiesis and thrombopoiesis have not been defined. ...
    • Fibroblast-Derived Induced Pluripotent Stem Cells Show No Common Retroviral Vector Insertions 

      Varas, Florencio; de Andres-Aguayo, Luisa; di Tullio, Alessandro; Pantano, Lorena; Notredame, Cedric; Graf, Thomas; Stadtfeld, Matthias; Maherali, Nimet Alia; Hochedlinger, Konrad (Wiley Subscription Services, Inc., A Wiley Company, 2009)
      Several laboratories have reported the reprogramming of mouse and human fibroblasts into pluripotent cells, using retroviruses carrying the Oct4, Sox2, Klf4, and c-Myc transcription factor genes. In these experiments the ...
    • The histone chaperone CAF-1 safeguards somatic cell identity 

      Cheloufi, Sihem; Elling, Ulrich; Hopfgartner, Barbara; Jung, Youngsook L; Murn, Jernej; Ninova, Maria; Hubmann, Maria; Badeaux, Aimee I; Ang, Cheen Euong; Tenen, Danielle; Wesche, Daniel J; Abazova, Nadezhda; Hogue, Max; Tasdemir, Nilgun; Brumbaugh, Justin; Rathert, Philipp; Jude, Julian; Ferrari, Francesco; Blanco, Andres; Fellner, Michaela; Wenzel, Daniel; Zinner, Marietta; Vidal, Simon E; Bell, Oliver; Stadtfeld, Matthias; Chang, Howard Y.; Almouzni, Genevieve; Lowe, Scott W; Rinn, John; Wernig, Marius; Aravin, Alexei; Shi, Yang; Park, Peter; Penninger, Josef M; Zuber, Johannes; Hochedlinger, Konrad (2016)
      Cellular differentiation involves profound remodeling of chromatic landscapes, yet the mechanisms by which somatic cell identity is subsequently maintained remain incompletely understood. To further elucidate regulatory ...
    • ISSCR 2013: Back to Bean Town 

      Brack, Andrew S.; Hochedlinger, Konrad (Elsevier, 2013)
      The International Society for Stem Cell Research 11th Annual Meeting was held in Boston in June 2013, bringing together just over 4000 attendees. An emphasis on therapeutic applications in many talks reflected the maturation ...
    • Lgr5-Positive Supporting Cells Generate New Hair Cells in the Postnatal Cochlea 

      Bramhall, Naomi F.; Shi, Fuxin; Arnold, Katrin; Hochedlinger, Konrad; Edge, Albert S.B. (Elsevier, 2014)
      Summary The prevalence of hearing loss after damage to the mammalian cochlea has been thought to be due to a lack of spontaneous regeneration of hair cells, the primary receptor cells for sound. Here, we show that supporting ...
    • Lineage conversion induced by pluripotency factors involves transient passage through an iPS cell stage 

      Bar-Nur, Ori; Verheul, Cassandra; Sommer, Andreia Gianotti; Brumbaugh, Justin; Schwarz, Benjamin A.; Lipchina, Inna; Huebner, Aaron J.; Mostoslavsky, Gustavo; Hochedlinger, Konrad (2016)
      Brief expression of pluripotency-associated factors such as Oct4, Klf4, Sox2 and c-Myc (OKSM), in combination with differentiation-inducing signals, was reported to trigger transdifferentiation of fibroblasts into other ...
    • Mediator Subunit Med28 Is Essential for Mouse Peri-Implantation Development and Pluripotency 

      Li, Lin; Walsh, Ryan M.; Wagh, Vilas; James, Marianne F.; Beauchamp, Roberta L.; Chang, Yuh-Shin; Gusella, James F.; Hochedlinger, Konrad; Ramesh, Vijaya (Public Library of Science, 2015)
      The multi-subunit mammalian Mediator complex acts as an integrator of transcriptional regulation by RNA Polymerase II, and has emerged as a master coordinator of development and cell fate determination. We previously ...
    • Nucleosomal occupancy changes locally over key regulatory regions during cell differentiation and reprogramming 

      West, Jason A.; Cook, April; Alver, Burak H.; Stadtfeld, Matthias; Deaton, Aimee; Hochedlinger, Konrad; Park, Peter J.; Tolstorukov, Michael Y.; Kingston, Robert E. (2014)
      Chromatin structure determines DNA accessibility. We compare nucleosome occupancy in mouse and human embryonic stem cells (ESCs), induced-pluripotent stem cells (iPSCs), and differentiated cell types using MNase-seq. To ...
    • Prolonged Mek1/2 suppression impairs the developmental potential of embryonic stem cells 

      Choi, Jiho; Huebner, Aaron; Clement, Kendell; Walsh, Ryan M.; Savol, Andrej J; Lin, Kaixuan; Gu, Hongcang; Di Stefano, Bruno; Brumbaugh, Justin; Kim, Sang-Yong; Sharif, Jafar; Rose, Christopher M.; Mohammad, Arman; Odajima, Junko; Charron, Jean; Shioda, Toshihiro; Gnirke, Andreas; Gygi, Steven P.; Koseki, Haruhiko; Sadreyev, Ruslan; Xiao, Andrew; Meissner, Alexander; Hochedlinger, Konrad (Springer Nature, 2017)
      Concomitant activation of the Wnt pathway and suppression of Mapk signalling by two small molecule inhibitors (2i) in the presence of leukaemia inhibitory factor (LIF) (hereafter termed 2i/L) induces a naive state in mouse ...
    • A Serial shRNA Screen for Roadblocks to Reprogramming Identifies the Protein Modifier SUMO2 

      Borkent, Marti; Bennett, Brian D.; Lackford, Brad; Bar-Nur, Ori; Brumbaugh, Justin; Wang, Li; Du, Ying; Fargo, David C.; Apostolou, Effie; Cheloufi, Sihem; Maherali, Nimet; Elledge, Stephen J.; Hu, Guang; Hochedlinger, Konrad (Elsevier, 2016)
      Summary The generation of induced pluripotent stem cells (iPSCs) from differentiated cells following forced expression of OCT4, KLF4, SOX2, and C-MYC (OKSM) is slow and inefficient, suggesting that transcription factors ...
    • Small molecules facilitate rapid and synchronous iPSC generation 

      Bar-Nur, Ori; Brumbaugh, Justin; Verheul, Cassandra; Apostolou, Effie; Pruteanu-Malinici, Iulian; Walsh, Ryan M.; Ramaswamy, Sridhar; Hochedlinger, Konrad (2014)
      The reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) upon overexpression of OCT4, KLF4, SOX2, and c-MYC (OKSM) provides a powerful system to interrogate basic mechanisms of cell fate change. ...
    • Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming 

      Deng, Jie; Shoemaker, Robert; Xie, Bin; Gore, Athurva; LeProust, Emily M; Antosiewicz-Bourget, Jessica; Egli, Dieter; Maherali, Nimet A; Park, In-Hyun; Yu, Junying; Daley, George Quentin; Eggan, Kevin Carl; Hochedlinger, Konrad; Thomson, James; Wang, Wei Li; Gao, Yuan; Zhang, Kun (Nature Publishing Group, 2009)
      Current DNA methylation assays are limited in the flexibility and efficiency of characterizing a large number of genomic targets. We report a method to specifically capture an arbitrary subset of genomic targets for ...