Browsing by Author "Cowan, Douglas"

Now showing items 1-13 of 13

    • Actin-dependent mitochondrial internalization in cardiomyocytes: evidence for rescue of mitochondrial function 

      Pacak, Christina A.; Preble, Janine M.; Kondo, Hiroshi; Seibel, Peter; Levitsky, Sidney; del Nido, Pedro J.; Cowan, Douglas B.; McCully, James D. (The Company of Biologists, 2015)
      Previously, we have demonstrated that the transplantation of viable, structurally intact, respiration competent mitochondria into the ischemic myocardium during early reperfusion significantly enhanced cardioprotection by ...

    • Diazoxide amelioration of myocardial injury and mitochondrial damage during cardiac surgery 

      McCully, James Donald; Wakiyama, Hidetaka; Cowan, Douglas Burr; Federman, Micheline; Parker, Robert A; Levitsky, Sidney (Elsevier BV, 2002)
      Background Recently, we have shown that the selective opening of mitochondrial ATP-sensitive potassium channels with diazoxide significantly decreases myocardial injury. The purpose of this study was to determine the ...

    • Differential Expression of Collagen Type V and XI ?-1 in Human Ascending Thoracic Aortic Aneurysms 

      Toumpoulis, Ioannis K.; Oxford, Julia Thom; Cowan, Douglas Burr; Anagnostopoulos, Constantine E.; Rokkas, Chris K.; Chamogeorgakis, Themistocles P.; Angouras, Dimitrios C.; Shemin, Richard J.; Navab, Mohamad; Ericsson, Maria; Federman, Micheline; Levitsky, Sidney; McCully, James Donald (Elsevier BV, 2009)
      Background The molecular mechanisms leading to ascending thoracic aortic aneurysms (ATAAs) remain unknown. We hypothesized that alterations in expression levels of specific fibrillar collagens occur during the aneurysmal ...

    • Dynamic Support Culture of Murine Skeletal Muscle-Derived Stem Cells Improves Their Cardiogenic Potential In Vitro 

      Neef, Klaus; Treskes, Philipp; Xu, Guoxing; Drey, Florian; Srinivasan, Sureshkumar Perumal; Saric, Tomo; Nembo, Erastus; Semmler, Judith; Nguemo, Filomain; Stamm, Christof; Cowan, Douglas B.; Deppe, Antje-Christin; Scherner, Maximilian; Wittwer, Thorsten; Hescheler, Jürgen; Wahlers, Thorsten; Choi, Yeong-Hoon (Hindawi Publishing Corporation, 2015)
      Ischemic heart disease is the main cause of death in western countries and its burden is increasing worldwide. It typically involves irreversible degeneration and loss of myocardial tissue leading to poor prognosis and ...

    • Fabrication of Myogenic Engineered Tissue Constructs 

      Pacak, Christina Ann; Cowan, Douglas Burr (Journal of Visualized Experiments, 2009)
      Despite the fact that electronic pacemakers are life-saving medical devices, their long-term performance in pediatric patients can be problematic owing to the restrictions imposed by a child's small size and their inevitable ...

    • Intracoronary Delivery of Mitochondria to the Ischemic Heart for Cardioprotection 

      Cowan, Douglas B.; Yao, Rouan; Akurathi, Vamsidhar; Snay, Erin R.; Thedsanamoorthy, Jerusha K.; Zurakowski, David; Ericsson, Maria; Friehs, Ingeborg; Wu, Yaotang; Levitsky, Sidney; del Nido, Pedro J.; Packard, Alan B.; McCully, James D. (Public Library of Science, 2016)
      We have previously shown that transplantation of autologously derived, respiration-competent mitochondria by direct injection into the heart following transient ischemia and reperfusion enhances cell viability and contractile ...

    • Microcarrier-Based Expansion of Adult Murine Side Population Stem Cells 

      Pacak, Christina Ann; Eddy, Mau-Thek; Woodhull, Lindsey; Wang, Kai-Roy; Alpatov, Ivan; Fullen, Shelby; Dowd, Rory P.; Choi, Yeong-Hoon; Cowan, Douglas Burr (Public Library of Science, 2013)
      The lack of reliable methods to efficiently isolate and propagate stem cell populations is a significant obstacle to the advancement of cell-based therapies for human diseases. One isolation technique is based on efflux ...

    • Mitochondrial transplantation for therapeutic use 

      McCully, James Donald; Levitsky, Sidney; Del Nido, Pedro J.; Cowan, Douglas Burr (Springer Science + Business Media, 2016)
      Mitochondria play a key role in the homeostasis of the vast majority of the body’s cells. In the myocardium where mitochondria constitute 30 % of the total myocardial cell volume, temporary attenuation or obstruction of ...

    • Myocardial Hypertrophy Overrides the Angiogenic Response to Hypoxia 

      Choi, Yeong-Hoon; Poutias, Dimitrios; Stamm, Christof; Cowan, Douglas Burr; Nathan, Meena; Del Nido, Pedro J.; McGowan, Francis Xavier (Public Library of Science, 2008)
      Background: Cyanosis and myocardial hypertrophy frequently occur in combination. Hypoxia or cyanosis can be potent inducers of angiogenesis, regulating the expression of hypoxia-inducible factors (HIF), vascular endothelial ...

    • Preconditioning of skeletal myoblast-based engineered tissue constructs enables functional coupling to myocardium in vivo 

      Treskes, Philipp; Neef, Klaus; Srinivasan, Sureshkumar Perumal; Halbach, Marcel; Stamm, Christof; Cowan, Douglas Burr; Scherner, Maximilian; Madershahian, Navid; Wittwer, Thorsten; Hescheler, Jürgen; Wahlers, Thorsten; Choi, Yeong-Hoon (Elsevier BV, 2015)
      Objective Skeletal myoblasts fuse to form functional syncytial myotubes as an integral part of the skeletal muscle. During this differentiation process, expression of proteins for mechanical and electrical integration is ...

    • Reduction and Redistribution of Gap and Adherens Junction Proteins After Ischemia and Reperfusion 

      Tansey, Erin E.; Kwaku, Kevin F.; Hammer, Peter; Cowan, Douglas Burr; Federman, Micheline; Levitsky, Sidney; McCully, James Donald (Elsevier BV, 2006)
      Background Previous studies have demonstrated that alterations in myocardial structure, consistent with tissue and sarcomere disruption as well as myofibril dissociation, occur after myocardial ischemia and reperfusion. ...

    • Selective opening of mitochondrial ATP-sensitive potassium channels during surgically induced myocardial ischemia decreases necrosis and apoptosis 

      Wakiyama, H; Cowan, Douglas Burr; Toyoda, Yoshiya; Federman, Miceline; Levitsky, Sidney; McCully, James Donald (Oxford University Press (OUP), 2002)
      Objective: Mitochondrial ATP-sensitive potassium channels have been proposed to be myoprotective. The relevance and specificity of this mechanism in cardiac surgery was unknown. The purpose of this study was to examine the ...

    • Superparamagnetic Iron Oxide Nanoparticles Function as a Long-Term, Multi-Modal Imaging Label for Non-Invasive Tracking of Implanted Progenitor Cells 

      Pacak, Christina A.; Hammer, Peter E.; MacKay, Allison A.; Dowd, Rory P.; Wang, Kai-Roy; Masuzawa, Akihiro; Sill, Bjoern; McCully, James D.; Cowan, Douglas B. (Public Library of Science, 2014)
      The purpose of this study was to determine the ability of superparamagnetic iron oxide (SPIO) nanoparticles to function as a long-term tracking label for multi-modal imaging of implanted engineered tissues containing ...