Browsing by Author "Esvelt, Kevin Michael"

Now showing items 1-9 of 9

    • CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering 

      Mali, Prashant; Aach, John; Stranges, P. Benjamin; Esvelt, Kevin M.; Moosburner, Mark; Kosuri, Sriram; Yang, Luhan; Church, George M. (2013)
      Prokaryotic type II CRISPR-Cas systems can be adapted to enable targeted genome modifications across a range of eukaryotes.1–7. Here we engineer this system to enable RNA-guided genome regulation in human cells by tethering ...

    • Complete Genome Sequences of T4-Like Bacteriophages RB3, RB5, RB6, RB7, RB9, RB10, RB27, RB33, RB55, RB59, and RB68 

      Yaung, Stephanie J.; Esvelt, Kevin M.; Church, George M. (American Society for Microbiology, 2015)
      T4-like bacteriophages have been explored for phage therapy and are model organisms for phage genomics and evolution. Here, we describe the sequencing of 11 T4-like phages. We found a high nucleotide similarity among the ...

    • Concerning RNA-guided gene drives for the alteration of wild populations 

      Esvelt, Kevin M; Smidler, Andrea L; Catteruccia, Flaminia; Church, George M (eLife Sciences Publications, Ltd, 2014)
      Gene drives may be capable of addressing ecological problems by altering entire populations of wild organisms, but their use has remained largely theoretical due to technical constraints. Here we consider the potential for ...

    • CRISPR/Cas9-Mediated Phage Resistance Is Not Impeded by the DNA Modifications of Phage T4 

      Yaung, Stephanie J.; Esvelt, Kevin M.; Church, George M. (Public Library of Science, 2014)
      Bacteria rely on two known DNA-level defenses against their bacteriophage predators: restriction-modification and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated (Cas) systems. Certain ...

    • Genome-scale engineering for systems and synthetic biology 

      Esvelt, Kevin Michael; Wang, Harris H (Nature Publishing Group, 2013)
      Genome-modification technologies enable the rational engineering and perturbation of biological systems. Historically, these methods have been limited to gene insertions or mutations at random or at a few pre-defined ...

    • Heritable genome editing in C. elegans via a CRISPR-Cas9 system 

      Friedland, Ari E.; Tzur, Yonatan B.; Esvelt, Kevin M.; Colaiácovo, Monica P.; Church, George M.; Calarco, John A. (2013)
      CRISPR-Cas systems have been used with single-guide RNAs for accurate gene disruption and conversion in multiple biological systems. Here we report the use of the endonuclease Cas9 to target genomic sequences in the C. ...

    • Inhibition of Bacterial Conjugation by Phage M13 and Its Protein g3p: Quantitative Analysis and Model 

      Lin, Abraham; Derr, Julien; Villanueva, Laura; Webber, Mark Alexander; Jimenez, Jose Ignacio; Vera, Pedro; Manapat, Michael; Esvelt, Kevin Michael; Liu, David Ruchien; Chen, Irene Ann (Public Library of Science, 2011)
      Conjugation is the main mode of horizontal gene transfer that spreads antibiotic resistance among bacteria. Strategies for inhibiting conjugation may be useful for preserving the effectiveness of antibiotics and preventing ...

    • Orthogonal Cas9 Proteins for RNA-Guided Gene Regulation and Editing 

      Esvelt, Kevin M.; Mali, Prashant; Braff, Jonathan L.; Moosburner, Mark; Yaung, Stephanie J.; Church, George M. (2013)
      The Cas9 protein from the Streptococcus pyogenes CRISPR-Cas immune system has been adapted for both RNA-guided genome editing and gene regulation in a variety of organisms, but can mediate only a single activity at a time ...

    • A system for the continuous directed evolution of biomolecules 

      Esvelt, Kevin Michael; Carlson, Jacob C.; Liu, David Ruchien (Nature Publishing Group, 2011)
      Laboratory evolution has generated many biomolecules with desired properties, but a single round of mutation, gene expression, screening or selection, and replication typically requires days or longer with frequent human ...