Browsing by Author "McMahon, Andrew P."
Now showing items 1-16 of 16
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Abnormal Hair Development and Apparent Follicular Transformation to Mammary Gland in the Absence of Hedgehog Signaling
Gritli-Linde, Amel; Linde, Anders; Hallberg, Kristina; Harfe, Brian D.; Reyahi, Azadeh; Kannius-Janson, Marie; Nilsson, Jeanette; Cobourne, Martyn T.; Sharpe, Paul T.; McMahon, Andrew P. (Elsevier, 2007)Summary: We show that removing the Shh signal tranducer Smoothened from skin epithelium secondarily results in excess Shh levels in the mesenchyme. Moreover, the phenotypes we observe reflect decreased epithelial Shh ... -
The cdx Genes and Retinoic Acid Control the Positioning and Segmentation of the Zebrafish Pronephros
Selleck, Rori; Song, Huai-Dong; Song, Anhua; Thisse, Bernard; Thisse, Christine; Mullins, Mary; Wingert, Rebecca Ann; Yu, Jingyi; Chen, Zhu; Zhou, Yi; McMahon, Andrew P.; Davidson, Alan J. (Public Library of Science, 2007)Kidney function depends on the nephron, which comprises a blood filter, a tubule that is subdivided into functionally distinct segments, and a collecting duct. How these regions arise during development is poorly understood. ... -
Cell Fate Decisions in Early Embryonic Development
Zhang, Xiaoxiao (2013-10-08)The basis of developmental biology lies in the idea of when and how cells decide to divide or to differentiate. Previous studies have established several signaling pathways that determine cell fate decisions, including ... -
An Embryonic Stem Cell-Based System for Rapid Analysis of Transcriptional Enhancers
Tsanov, Kaloyan M; Nishi, Yuichi; Peterson, Kevin A; Liu, Jing; Baetscher, Manfred; McMahon, Andrew P. (Wiley Periodicals, Inc., 2012)With the growing use of genome-wide screens for cis-regulatory elements, there is a pressing need for platforms that enable fast and cost-effective experimental validation of identified hits in relevant developmental and ... -
An ES Cell System for Rapid, Spatial and Temporal Analysis of Gene Function in vitro and in vivo
Mao, Junhao; Barrow, Jeffery; McMahon, Jill Ann; Vaughan, Joe; McMahon, Andrew P. (Oxford University Press, 2005)We describe a versatile genetic system for rapid analysis of mammalian gene function. In this, loss of reporter activity in a novel embryonic stem (ES) cell line enables rapid identification of targeting to the ubiquitously ... -
A Genome-Wide Screen to Identify Transcription Factors Expressed in Pelvic Ganglia of the Lower Urinary Tract
Wiese, Carrie B.; Ireland, Sara; Fleming, Nicole L.; Georgas, Kylie; Chiu, Han Sheng; Brennan, Jane; Armstrong, Jane; Little, Melissa H.; Southard-Smith, E. Michelle; Yu, Jing; Valerius, Michael Todd; McMahon, Andrew P. (Frontiers Research Foundation, 2012)Relative positions of neurons within mature murine pelvic ganglia based on expression of neurotransmitters have been described. However the spatial organization of developing innervation in the murine urogenital tract (UGT) ... -
Germ Cells Are Not Required to Establish the Female Pathway in Mouse Fetal Gonads
Maatouk, Danielle M.; Mork, Lindsey; Hinson, Ashley; Kobayashi, Akio; McMahon, Andrew P.; Capel, Blanche (Public Library of Science, 2012)The fetal gonad is composed of a mixture of somatic cell lineages and germ cells. The fate of the gonad, male or female, is determined by a population of somatic cells that differentiate into Sertoli or granulosa cells and ... -
Gli Transcriptional Activity Is Essential for Kras-Induced Pancreatic Tumorigenesis and Regulates IKBKE/NF-\(\kappa\)B Activity in the Tumor Epithelium
Rajurkar, Mihir; de Jesus-Monge, Wilfredo E.; Driscoll, David R.; Appleman, Victoria A.; Huang, He; Cotton, Jennifer; Klimstra, David; Zhu, Lihua; Simin, Karl; Xu, Lan; McMahon, Andrew P.; Lewis, Brian; Mao, Junhao (National Academy of Science, 2012)Pancreatic ductal adenocarcinoma (PDAC), one of the most aggressive human malignances, is thought to be initiated by KRAS activation. Here we find that transcriptional activation mediated by the Gli family of transcription ... -
Glucocorticoid Compounds Modify Smoothened Localization and Hedgehog Pathway Activity
McMahon, Andrew P.; Davidow, Lance Steven; Wang, Yu; Blanchard, Joel; Lam, Kelvin; Xu, Ke; Oza, Vatsal U.; Woo Yoo, Jin; Ng, Jessica; Curran, Tom; Rubin, Lee; Arvanites, Anthony (Elsevier, 2012)The Hedgehog signaling pathway is linked to a variety of diseases, notably a range of cancers. The first generation of drug screens identified Smoothened (Smo), a membrane protein essential for signaling, as an attractive ... -
Identification of Molecular Compartments and Genetic Circuitry in the Developing Mammalian Kidney
Duah, Mary; Staser, Karl; Valerius, Michael Todd; Hansard, Jennifer K.; Guo, Jin-jin; McMahon, Jill Ann; Vaughan, Joseph E.; Faria, Diane; Georgas, Kylie; Rumballe, Bree; Ren, Qun; Krautzberger, A. Michaela; Junker, Jan P.; Thiagarajan, Rathi D.; Machanick, Philip; Gray, Paul A.; van Oudenaarden, Alexander; Rowitch, David H.; Stiles, Charles Dean; Ma, Qiufu; Grimmond, Sean M.; Bailey, Timothy L.; Little, Melissa H.; McMahon, Andrew P.; Yu, Jing (Company of Biologists, 2012)Lengthy developmental programs generate cell diversity within an organotypic framework, enabling the later physiological actions of each organ system. Cell identity, cell diversity and cell function are determined by cell ... -
Investigation of the Molecular and Cellular Basis of Patterning, Morphogenesis, and Tubule Interconnections during Mammalian Kidney Development
Kao, Robert (2013-02-19)The formation of a continuous tubular network in the mammalian urinary system requires the interconnection of two epithelial populations with distinct cellular origins. The proximal component of the renal network is the ... -
Motor Neurons with Axial Muscle Projections Specified by Wnt4/5 Signaling
Agalliu, Dritan; Takada, Shinji; Agalliu, Ilir; Jessell, Thomas M.; McMahon, Andrew P. (Cell Press, 2009)Summary Axial muscles are innervated by motor neurons of the median motor column (MMC). In contrast to the segmentally restricted motor columns that innervate limb, body wall, and neuronal targets, MMC neurons are generated ... -
Neuroepithelial Body Microenvironment Is a Niche for a Distinct Subset of Clara-Like Precursors in the Developing Airways
Guha, Arjun; Vasconcelos, Michelle; Cai, Yan; Yoneda, Mitsuhiro; Hinds, Anne; Qian, Jun; Li, Guihua; Dickel, Lauren; Johnson, Jane E.; Kimura, Shioko; Guo, Jinjin; McMahon, Jill; McMahon, Andrew; Cardoso, Wellington V. (Proceedings of the National Academy of Sciences, 2012)Clara cells of mammalian airways have multiple functions and are morphologically heterogeneous. Although Notch signaling is essential for the development of these cells, it is unclear how Notch influences Clara cell ... -
Selective Identification of Hedgehog Pathway Antagonists by Direct Analysis of Smoothened Ciliary Translocation
Wang, Yu; Arvanites, Anthony C.; Davidow, Lance Steven; Blanchard, Joel; Lam, Kelvin; Yoo, Jin Woo; Coy, Shannon; Rubin, Lee L.; McMahon, Andrew P. (American Chemical Society, 2012-05-11)Hedgehog (Hh) signaling promotes tumorigenesis. The accumulation of the membrane protein Smoothened (Smo) within the primary cilium (PC) is a key event in Hh signal transduction, and many pharmacological inhibitors identified ... -
Sox17 promotes differentiation in mouse embryonic stem cells by directly regulating extraembryonic gene expression and indirectly antagonizing self-renewal
Niakan, K. K.; Ji, H.; Maehr, R.; Vokes, S. A.; Rodolfa, K. T.; Sherwood, Richard Irving; Yamaki, M.; Dimos, J. T.; Chen, A. E.; Melton, Douglas A.; McMahon, Andrew P.; Eggan, Kevin Carl (Cold Spring Harbor Laboratory Press, 2010)In embryonic stem (ES) cells, a well-characterized transcriptional network promotes pluripotency and represses gene expression required for differentiation. In comparison, the transcriptional networks that promote ... -
Using Mechanistic Bayesian Networks to Identify Downstream Targets of the Sonic Hedgehog Pathway
Shah, Abhik; Tenzen, Toyoaki; McMahon, Andrew P.; Woolf, Peter J (BioMed Central, 2009)Background: The topology of a biological pathway provides clues as to how a pathway operates, but rationally using this topology information with observed gene expression data remains a challenge. Results: We introduce a ...