Now showing items 1-16 of 16

    • Altered drug susceptibility during host adaptation of a Plasmodium falciparum strain in a non-human primate model 

      Obaldía III, Nicanor; Dow, Geoffrey S.; Gerena, Lucia; Kyle, Dennis; Otero, William; Mantel, Pierre-Yves; Baro, Nicholas; Daniels, Rachel; Mukherjee, Angana; Childs, Lauren M.; Buckee, Caroline; Duraisingh, Manoj T.; Volkman, Sarah K.; Wirth, Dyann F.; Marti, Matthias (Nature Publishing Group, 2016)
      Infections with Plasmodium falciparum, the most pathogenic of the Plasmodium species affecting man, have been reduced in part due to artemisinin-based combination therapies. However, artemisinin resistant parasites have ...
    • Ancient human sialic acid variant restricts an emerging zoonotic malaria parasite 

      Dankwa, Selasi; Lim, Caeul; Bei, Amy K.; Jiang, Rays H. Y.; Abshire, James R.; Patel, Saurabh D.; Goldberg, Jonathan M.; Moreno, Yovany; Kono, Maya; Niles, Jacquin C.; Duraisingh, Manoj T. (Nature Publishing Group, 2016)
      Plasmodium knowlesi is a zoonotic parasite transmitted from macaques causing malaria in humans in Southeast Asia. Plasmodium parasites bind to red blood cell (RBC) surface receptors, many of which are sialylated. While ...
    • Characterization and Structural Studies of the Plasmodium Falciparum Ubiquitin and Nedd8 Hydrolase UCHL3 

      Artavanis-Tsakonas, Katerina; Weihofen, Wilhelm Andreas; Antos, John M.; Coleman, Bradley Ian; Comeaux, Christy Ann; Duraisingh, Manoj T.; Gaudet, Rachelle; Ploegh, Hidde L. (American Society for Biochemistry and Molecular Biology, 2010)
      Like their human hosts, Plasmodium falciparum parasites rely on the ubiquitin-proteasome system for survival. We previously identified PfUCHL3, a deubiquitinating enzyme, and here we characterize its activity and changes ...
    • Demographic and clinical profiles of Plasmodium falciparum and Plasmodium vivax patients at a tertiary care centre in southwestern India 

      Chery, Laura; Maki, Jennifer N.; Mascarenhas, Anjali; Walke, Jayashri T.; Gawas, Pooja; Almeida, Anvily; Fernandes, Mezia; Vaz, Marina; Ramanan, Rakesh; Shirodkar, Diksha; Bernabeu, Maria; Manoharan, Suresh Kumar; Pereira, Ligia; Dash, Rashmi; Sharma, Ambika; Shaik, Riaz Basha; Chakrabarti, Rimi; Babar, Prasad; White, John; Mudeppa, Devaraja G.; Kumar, Shiva; Zuo, Wenyun; Skillman, Kristen M.; Kanjee, Usheer; Lim, Caeul; Shaw-Saliba, Kathryn; Kumar, Ashwani; Valecha, Neena; Jindal, V. N.; Khandeparkar, Anar; Naik, Pradeep; Amonkar, Sunanda; Duraisingh, Manoj T.; Tuljapurkar, Shripad; Smith, Joseph D.; Dubhashi, Nagesh; Pinto, Roque G. W.; Silveria, Maria; Gomes, Edwin; Rathod, Pradipsinh K. (BioMed Central, 2016)
      Background: Malaria remains an important cause of morbidity and mortality in India. Though many comprehensive studies have been carried out in Africa and Southeast Asia to characterize and examine determinants of Plasmodium ...
    • Dietary restriction protects against experimental cerebral malaria via leptin modulation and T cell mTORC1 suppression 

      Mejia, Pedro; Treviño-Villarreal, J. Humberto; Hine, Christopher; Harputlugil, Eylul; Lang, Samantha; Calay, Ediz; Rogers, Rick; Wirth, Dyann; Duraisingh, Manoj T.; Mitchell, James R. (2014)
      Host nutrition can affect the outcome of parasitic diseases through metabolic effects on host immunity and/or the parasite. Here we show that modulation of mouse immunometabolism through brief restriction of food intake ...
    • Enrichment of Reticulocytes from Whole Blood using Aqueous Multiphase Systems of Polymers 

      Kumar, Ashok Ashwin; Lim, Caeul; Moreno, Yovany; Mace, Charles R.; Syed, Abeer; Van Tyne, Daria Natalie; Wirth, Dyann F.; Duraisingh, Manoj T.; Whitesides, George McClelland (Wiley-Blackwell, 2014)
      This paper demonstrates the enrichment of reticulocytes by centrifuging whole blood through aqueous multiphase systems (AMPSs)—immiscible phases of solutions of polymers that form step-gradients in density. The interfaces ...
    • Evidence for spleen dysfunction in malaria-HIV co-infection in a subset of pediatric patients 

      Joice, Regina; Frantzreb, Charles; Pradham, Alana; Seydel, Karl B.; Kamiza, Steve; Wirth, Dyann F.; Duraisingh, Manoj T.; Molyneux, Malcolm E; Taylor, Terrie E.; Marti, Matthias; Milner, Danny A. (2015)
      The spleen has an important role in the clearance of malaria parasites, and the role of HIV co-infection on this process is yet to be described. Using a combination of histological and molecular methods, we systematically ...
    • Evidence-Based Annotation of the Malaria Parasite's Genome Using Comparative Expression Profiling 

      Zhou, Yingyao; Ramachandran, Vandana; Kumar, Kota Arun; Westenberger, Scott; Refour, Phillippe; Zhou, Bin; Li, Fengwu; Young, Jason A.; Chen, Kaisheng; Plouffe, David; Henson, Kerstin; Nussenzweig, Victor; Carlton, Jane; Vinetz, Joseph M.; Winzeler, Elizabeth A.; Duraisingh, Manoj T.; Hofmann, Oliver Marc (Public Library of Science, 2008)
      A fundamental problem in systems biology and whole genome sequence analysis is how to infer functions for the many uncharacterized proteins that are identified, whether they are conserved across organisms of different phyla ...
    • Functional Analysis of Sirtuin Genes in Multiple Plasmodium falciparum Strains 

      Merrick, Catherine J.; Jiang, Rays H. Y.; Skillman, Kristen M.; Samarakoon, Upeka; Moore, Rachel M.; Dzikowski, Ron; Ferdig, Michael T.; Duraisingh, Manoj T. (Public Library of Science, 2015)
      Plasmodium falciparum, the causative agent of severe human malaria, employs antigenic variation to avoid host immunity. Antigenic variation is achieved by transcriptional switching amongst polymorphic var genes, enforced ...
    • A Global Transcriptional Analysis of Plasmodium Falciparum Malaria Reveals A Novel Family of Telomere-Associated lncRNAs 

      Broadbent, Kate Mariel; Park, Daniel John; Wolf, Ashley Robin; Van Tyne, Daria Natalie; Sims, Jennifer Sung; Ribacke, Ulf Erik; Cooke, Sarah Volkman; Duraisingh, Manoj T.; Wirth, Dyann Fergus; Sabeti, Pardis Christine; Rinn, John L (BioMed Central, 2011)
      Background: Mounting evidence suggests a major role for epigenetic feedback in Plasmodium falciparum transcriptional regulation. Long non-coding RNAs (lncRNAs) have recently emerged as a new paradigm in epigenetic ...
    • Identification and Characterization of Small Molecule Inhibitors of a Class I Histone Deacetylase from Plasmodium falciparum 

      Nguyen, Cokey; Urgaonkar, Sameer; Cortese, Joseph; Barker, Robert H.; Greenberg, Edward; Tang, Weiping; Patel, Vishal; Mazitschek, Ralph; Coleman, Bradley Ian; Bradner, James Elliott; Schreiber, Stuart L.; Duraisingh, Manoj T.; Wirth, Dyann Fergus; Clardy, Jon C. (American Chemical Society, 2009)
      A library of approximately 2000 small molecules biased toward inhibition of histone deacetylases was assayed for antimalarial activity in a high-throughput P. falciparum viability assay. Active compounds were cross-analyzed ...
    • Independent Origin and Global Distribution of Distinct Plasmodium vivax Duffy Binding Protein Gene Duplications 

      Hostetler, Jessica B.; Lo, Eugenia; Kanjee, Usheer; Amaratunga, Chanaki; Suon, Seila; Sreng, Sokunthea; Mao, Sivanna; Yewhalaw, Delenasaw; Mascarenhas, Anjali; Kwiatkowski, Dominic P.; Ferreira, Marcelo U.; Rathod, Pradipsinh K.; Yan, Guiyun; Fairhurst, Rick M.; Duraisingh, Manoj T.; Rayner, Julian C. (Public Library of Science, 2016)
      Background: Plasmodium vivax causes the majority of malaria episodes outside Africa, but remains a relatively understudied pathogen. The pathology of P. vivax infection depends critically on the parasite’s ability to ...
    • Infected erythrocyte-derived extracellular vesicles alter vascular function via regulatory Ago2-miRNA complexes in malaria 

      Mantel, Pierre-Yves; Hjelmqvist, Daisy; Walch, Michael; Kharoubi-Hess, Solange; Nilsson, Sandra; Ravel, Deepali; Ribeiro, Marina; Grüring, Christof; Ma, Siyuan; Padmanabhan, Prasad; Trachtenberg, Alexander; Ankarklev, Johan; Brancucci, Nicolas M.; Huttenhower, Curtis; Duraisingh, Manoj T.; Ghiran, Ionita; Kuo, Winston P.; Filgueira, Luis; Martinelli, Roberta; Marti, Matthias (Nature Publishing Group, 2016)
      Malaria remains one of the greatest public health challenges worldwide, particularly in sub-Saharan Africa. The clinical outcome of individuals infected with Plasmodium falciparum parasites depends on many factors including ...
    • Insights into an Optimization of Plasmodium vivax Sal-1 In Vitro Culture: The Aotus Primate Model 

      Shaw-Saliba, Kathryn; Thomson-Luque, Richard; Obaldía, Nicanor; Nuñez, Marlon; Dutary, Sahir; Lim, Caeul; Barnes, Samantha; Kocken, Clemens H. M.; Duraisingh, Manoj T.; Adams, John H.; Pasini, Erica M. (Public Library of Science, 2016)
      Malaria is one of the most significant tropical diseases, and of the Plasmodium species that cause human malaria, P. vivax is the most geographically widespread. However, P. vivax remains a relatively neglected human ...
    • Investigating the Pathogenesis of Severe Malaria: A Multidisciplinary and Cross-Geographical Approach 

      Wassmer, Samuel C.; Taylor, Terrie E.; Rathod, Pradipsinh K.; Mishra, Saroj K.; Mohanty, Sanjib; Arevalo-Herrera, Myriam; Duraisingh, Manoj T.; Smith, Joseph D. (The American Society of Tropical Medicine and Hygiene, 2015)
      More than a century after the discovery of Plasmodium spp. parasites, the pathogenesis of severe malaria is still not well understood. The majority of malaria cases are caused by Plasmodium falciparum and Plasmodium vivax, ...
    • Optimization of flow cytometric detection and cell sorting of transgenic Plasmodium parasites using interchangeable optical filters 

      Vorobjev, Ivan A; Buchholz, Kathrin; Prabhat, Prashant; Ketman, Kenneth James; Egan, Elizabeth Sullivan; Marti, Matthias; Duraisingh, Manoj T.; Barteneva, Natasha S (BioMed Central, 2012)
      Background: Malaria remains a major cause of morbidity and mortality worldwide. Flow cytometry-based assays that take advantage of fluorescent protein (FP)-expressing malaria parasites have proven to be valuable tools for ...