Browsing by Author "Thompson, David"
Now showing items 1-5 of 5
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Development of Methods for Protein Delivery and the Directed Evolution of Recombinases
Thompson, David Brandon (2014-10-22)As a class, protein-based therapeutics offer tremendous advantages over traditional small molecule drugs. Due to their sizes and folding energies, proteins are ideal for catalyzing chemical reactions, and can bind tightly ... -
Efficient Delivery of Genome-Editing Proteins In Vitro and In Vivo
Zuris, John A.; Thompson, David B.; Shu, Yilai; Guilinger, John P.; Bessen, Jeffrey L.; Hu, Johnny H.; Maeder, Morgan L.; Joung, J. Keith; Chen, Zheng-Yi; Liu, David R. (2014)Efficient intracellular delivery of proteins is needed to fully realize the potential of protein therapeutics. Current methods of protein delivery commonly suffer from low tolerance for serum, poor endosomal escape, and ... -
Mammalian Cell Penetration,siRNA Transfection, and DNA Transfection by Supercharged Green Fluorescent Proteins
McNaughton, Brian R.; Cronican, James Joseph; Thompson, David Brandon; Liu, David Ruchien (National Academy of Sciences, 2009)Nucleic acid reagents, including small interfering RNA (siRNA) and plasmid DNA, are important tools for the study of mammalian cells and are promising starting points for the development of new therapeutic agents. Realizing ... -
A programmable Cas9-serine recombinase fusion protein that operates on DNA sequences in mammalian cells
Chaikind, Brian; Bessen, Jeffrey L.; Thompson, David B.; Hu, Johnny H.; Liu, David R. (Oxford University Press, 2016)We describe the development of ‘recCas9’, an RNA-programmed small serine recombinase that functions in mammalian cells. We fused a catalytically inactive dCas9 to the catalytic domain of Gin recombinase using an optimized ... -
Small Molecule-Triggered Cas9 Protein with Improved Genome-Editing Specificity
Davis, Kevin M.; Pattanayak, Vikram; Thompson, David B.; Zuris, John A.; Liu, David R. (2015)Directly modulating the activity of genome-editing proteins has the potential to increase their specificity by reducing activity following target locus modification. We developed Cas9 nucleases that are activated by the ...