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    • Efficient, Continuous Mutagenesis in Human Cells Using a Pseudo-Random DNA Editor 

      Chen, Haiqi; Liu, Sophia; Padula, Samuel; Lesman, Daniel; Griswold, Kettner; Lin, Allen; Zhao, Tongtong; Marshall, Jamie; Chen, Fei (Springer Science and Business Media LLC, 2019-12-16)
      Here, we describe TRACE, a method that enables continuous, targeted mutagenesis in human cells using a cytidine deaminase fused to T7 RNA polymerase. TRACE induces high rates of mutagenesis over multiple cell generations ...