Browsing by Author "Shah, Jagesh"

Now showing items 1-16 of 16

    • ANKS6 is the critical activator of NEK8 kinase in embryonic situs determination and organ patterning 

      Czarnecki, Peter G.; Gabriel, George C.; Manning, Danielle K.; Sergeev, Mikhail; Lemke, Kristi; Klena, Nikolai T.; Liu, Xiaoqin; Chen, Yu; Li, You; San Agustin, Jovenal T.; Garnaas, Maija K.; Francis, Richard J.; Tobita, Kimimasa; Goessling, Wolfram; Pazour, Gregory J.; Lo, Cecilia W.; Beier, David R.; Shah, Jagesh V. (2015)
      The ciliary kinase NEK8 plays a critical role in situs determination and cystic kidney disease, yet its exact function remains unknown. In this study we identify ANKS6 as a target and activator of NEK8. ANKS6 requires NEK8 ...

    • A BioBrick compatible strategy for genetic modification of plants 

      Boyle, Patrick M; Burrill, Devin Rene; Inniss, Mara Christine; Agapakis, Christina M; Deardon, Aaron; dewerd, Jonathan G; Gedeon, Michael A; Quinn, Jacqueline Y; Paull, Morgan L; Raman, Anugraha M; Theilmann, Mark R; Wang, Lu; Winn, Julia C; Medvedik, Oliver; Schellenberg, Kurt William; Haynes, Karmella; Viel, Alain; Brenner, Tamara Jane; Church, George McDonald; Shah, Jagesh V.; Silver, Pamela A. (BioMed Central, 2012)
      Background: Plant biotechnology can be leveraged to produce food, fuel, medicine, and materials. Standardized methods advocated by the synthetic biology community can accelerate the plant design cycle, ultimately making ...

    • Cells in Tight Spaces: The Role of Cell Shape in Cell Function 

      Shah, Jagesh V. (The Rockefeller University Press, 2010)
      In this issue, Pitaval et al. (2010. J. Cell Biol. doi:10.1083/jcb.201004003) demonstrate that cell geometry can regulate the elaboration of a primary cilium. Their findings and approaches are part of a historical line of ...

    • Chemical and Physical Determinants of Cell Migration 

      Prentice Mott, Harrison Valentine (2014-06-20)
      The phenomenon of directed cell motion in response to external directional cues has drawn significant interest for more than a century, with the first recorded observations of bacterial chemotaxis at the end of the 19th ...

    • Chromosome Tips Damaged in Anaphase Inhibit Cytokinesis 

      Baker, Norman M.; Zeitlin, Samantha G.; Shi, Linda Z.; Shah, Jagesh V.; Berns, Michael W. (Public Library of Science, 2010)
      Genome maintenance is ensured by a variety of biochemical sensors and pathways that repair accumulated damage. During mitosis, the mechanisms that sense and resolve DNA damage remain elusive. Studies have demonstrated that ...

    • High-Affinity Accumulation of a Maytansinoid in Cells via Weak Tubulin Interaction 

      Goldmacher, Victor S.; Audette, Charlene A.; Guan, Yinghua; Sidhom, Eriene-Heidi; Shah, Jagesh V.; Whiteman, Kathleen R.; Kovtun, Yelena V. (Public Library of Science, 2015)
      The microtubule-targeting maytansinoids accumulate in cells and induce mitotic arrest at 250- to 1000-fold lower concentrations than those required for their association with tubulin or microtubules. To identify the ...

    • Impact of Histone H4 Lysine 20 Methylation on 53BP1 Responses to Chromosomal Double Strand Breaks 

      Hartlerode, Andrea J.; Guan, Yinghua; Rajendran, Anbazhagan; Ura, Kiyoe; Schotta, Gunnar; Xie, Anyong; Shah, Jagesh V.; Scully, Ralph (Public Library of Science, 2012)
      Recruitment of 53BP1 to chromatin flanking double strand breaks (DSBs) requires γH2AX/MDC1/RNF8-dependent ubiquitination of chromatin and interaction of 53BP1 with histone H4 methylated on lysine 20 (H4K20me). Several ...

    • Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore 

      Cojoc, Gheorghe; Roscioli, Emanuele; Zhang, Lijuan; García-Ulloa, Alfonso; Shah, Jagesh V.; Berns, Michael W.; Pavin, Nenad; Cimini, Daniela; Tolić, Iva M.; Gregan, Juraj (The Rockefeller University Press, 2016)
      Accurate chromosome segregation depends on proper kinetochore–microtubule attachment. Upon microtubule interaction, kinetochores are subjected to forces generated by the microtubules. In this work, we used laser ablation ...

    • Live-cell multiphoton fluorescence correlation spectroscopy with an improved large Stokes shift fluorescent protein 

      Guan, Yinghua; Meurer, Matthias; Raghavan, Sarada; Rebane, Aleksander; Lindquist, Jake R.; Santos, Sofia; Kats, Ilia; Davidson, Michael W.; Mazitschek, Ralph; Hughes, Thomas E.; Drobizhev, Mikhail; Knop, Michael; Shah, Jagesh V. (The American Society for Cell Biology, 2015)
      We report an improved variant of mKeima, a monomeric long Stokes shift red fluorescent protein, hmKeima8.5. The increased intracellular brightness and large Stokes shift (∼180 nm) make it an excellent partner with teal ...

    • LRF maintains genome integrity by regulating the non-homologous end joining pathway of DNA repair 

      Liu, Xue-Song; Chandramouly, Gurushankar; Rass, Emilie; Guan, Yinghua; Wang, Guocan; Hobbs, Robin M.; Rajendran, Anbazhagan; Xie, Anyong; Shah, Jagesh V.; Davis, Anthony J.; Scully, Ralph; Lunardi, Andrea; Pandolfi, Pier Paolo (Nature Pub. Group, 2015)
      Leukemia/lymphoma-related factor (LRF) is a POZ/BTB and Krüppel (POK) transcriptional repressor characterized by context-dependent key roles in cell fate decision and tumorigenesis. Here we demonstrate an unexpected ...

    • Monoalkoxy BODIPYs—A Fluorophore Class for Bioimaging 

      Courtis, Alexandra M.; Santos, Sofia A.; Guan, Yinghua; Hendricks, J. Adam; Ghosh, Balaram; Szantai-Kis, D. Miklos; Reis, Surya A.; Shah, Jagesh V.; Mazitschek, Ralph (American Chemical Society, 2014)
      Small molecule fluorophores are indispensable tools for modern biomedical imaging techniques. In this report, we present the development of a new class of BODIPY dyes based on an alkoxy-fluoro-boron-dipyrromethene core. ...

    • The quantitative architecture of centromeric chromatin 

      Bodor, Dani L; Mata, João F; Sergeev, Mikhail; David, Ana Filipa; Salimian, Kevan J; Panchenko, Tanya; Cleveland, Don W; Black, Ben E; Shah, Jagesh V; Jansen, Lars ET (eLife Sciences Publications, Ltd, 2014)
      The centromere, responsible for chromosome segregation during mitosis, is epigenetically defined by CENP-A containing chromatin. The amount of centromeric CENP-A has direct implications for both the architecture and ...

    • A Quantitative Systems View of the Spindle Assembly Checkpoint 

      Ciliberto, Andrea; Shah, Jagesh V. (Nature Publishing Group, 2009)
      The idle assembly checkpoint acts to delay chromosome segregation until all duplicated sister chromatids are captured by the mitotic spindle. This pathway ensures that each daughter cell receives a complete copy of the ...

    • Reduction of ciliary length through pharmacologic or genetic inhibition of CDK5 attenuates polycystic kidney disease in a model of nephronophthisis 

      Husson, Hervé; Moreno, Sarah; Smith, Laurie A.; Smith, Mandy M.; Russo, Ryan J.; Pitstick, Rose; Sergeev, Mikhail; Ledbetter, Steven R.; Bukanov, Nikolay O.; Lane, Monica; Zhang, Kate; Billot, Katy; Carlson, George; Shah, Jagesh; Meijer, Laurent; Beier, David R.; Ibraghimov-Beskrovnaya, Oxana (Oxford University Press, 2016)
      Polycystic kidney diseases (PKDs) comprise a subgroup of ciliopathies characterized by the formation of fluid-filled kidney cysts and progression to end-stage renal disease. A mechanistic understanding of cystogenesis is ...

    • Spindle checkpoint silencing: ensuring rapid and concerted anaphase onset 

      Hardwick, Kevin G; Shah, Jagesh V. (Faculty of 1000 Ltd, 2010)
      The spindle checkpoint delays anaphase onset in the presence of defective kinetochore-microtubule attachments. Such delays can last for just a few minutes or several hours, but very shortly after all chromosomes achieve ...

    • Systematic VCP-UBXD Adaptor Network Proteomics Identifies a Role for UBXN10 in Regulating Ciliogenesis 

      Raman, Malavika; Sergeev, Mikhail; Garnaas, Maija; Lydeard, John R.; Huttlin, Edward L.; Goessling, Wolfram; Shah, Jagesh V.; Harper, J. Wade (2015)
      The AAA-ATPase VCP (also known as p97 or CDC48) uses ATP hydrolysis to “segregate” ubiquitinated proteins from their binding partners. VCP acts via UBX-domain containing adaptors that provide target specificity, but targets ...