Now showing items 1-10 of 10

    • Bacterial protein translocation requires only one copy of the SecY complex in vivo 

      Park, Eunyong; Rapoport, Tom A. (The Rockefeller University Press, 2012)
      The transport of proteins across the plasma membrane in bacteria requires a channel formed from the SecY complex, which cooperates with either a translating ribosome in cotranslational translocation or the SecA ATPase in ...
    • Crystal structure of a substrate-engaged SecY protein-translocation channel 

      Li, Long; Park, Eunyong; Ling, JingJing; Ingram, Jessica; Ploegh, Hidde; Rapoport, Tom A. (2016)
      Hydrophobic signal sequences target secretory polypeptides to a protein-conducting channel formed by a heterotrimeric membrane protein complex, the prokaryotic SecY or eukaryotic Sec61 complex. How signal sequences are ...
    • Data publication with the structural biology data grid supports live analysis 

      Meyer, Peter A.; Socias, Stephanie; Key, Jason; Ransey, Elizabeth; Tjon, Emily C.; Buschiazzo, Alejandro; Lei, Ming; Botka, Chris; Withrow, James; Neau, David; Rajashankar, Kanagalaghatta; Anderson, Karen S.; Baxter, Richard H.; Blacklow, Stephen C.; Boggon, Titus J.; Bonvin, Alexandre M. J. J.; Borek, Dominika; Brett, Tom J.; Caflisch, Amedeo; Chang, Chung-I; Chazin, Walter J.; Corbett, Kevin D.; Cosgrove, Michael S.; Crosson, Sean; Dhe-Paganon, Sirano; Di Cera, Enrico; Drennan, Catherine L.; Eck, Michael J.; Eichman, Brandt F.; Fan, Qing R.; Ferré-D'Amaré, Adrian R.; Christopher Fromme, J.; Garcia, K. Christopher; Gaudet, Rachelle; Gong, Peng; Harrison, Stephen C.; Heldwein, Ekaterina E.; Jia, Zongchao; Keenan, Robert J.; Kruse, Andrew C.; Kvansakul, Marc; McLellan, Jason S.; Modis, Yorgo; Nam, Yunsun; Otwinowski, Zbyszek; Pai, Emil F.; Pereira, Pedro José Barbosa; Petosa, Carlo; Raman, C. S.; Rapoport, Tom A.; Roll-Mecak, Antonina; Rosen, Michael K.; Rudenko, Gabby; Schlessinger, Joseph; Schwartz, Thomas U.; Shamoo, Yousif; Sondermann, Holger; Tao, Yizhi J.; Tolia, Niraj H.; Tsodikov, Oleg V.; Westover, Kenneth D.; Wu, Hao; Foster, Ian; Fraser, James S.; Maia, Filipe R. N C.; Gonen, Tamir; Kirchhausen, Tom; Diederichs, Kay; Crosas, Mercè; Sliz, Piotr (Nature Publishing Group, 2016)
      Access to experimental X-ray diffraction image data is fundamental for validation and reproduction of macromolecular models and indispensable for development of structural biology processing methods. Here, we established ...
    • The dynamin-like GTPase Sey1p mediates homotypic ER fusion in S. cerevisiae 

      Anwar, Kamran; Klemm, Robin; Condon, Amanda; Severin, Katharina N.; Zhang, Miaoxing; Ghirlando, Rodolfo; Hu, Junjie; Rapoport, Tom A.; Prinz, William A. (The Rockefeller University Press, 2012)
      The endoplasmic reticulum (ER) forms a network of tubules and sheets that requires homotypic membrane fusion to be maintained. In metazoans, this process is mediated by dynamin-like guanosine triphosphatases (GTPases) ...
    • Mechanism of endoplasmic reticulum membrane fusion mediated by the Atlastin GTPase 

      Liu, Tina Yu (2014-10-21)
      How organelles acquire their unique shapes is a fundamental question of cell biology. The peripheral endoplasmic reticulum (ER) consists of a vast network of membrane sheets and tubules, the formation of which requires ...
    • Mechanistic Studies of SecY-Mediated Protein Translocation in Intact Escherichia coli Cells 

      Park, Eunyong (2013-02-12)
      During the synthesis of secretory and membrane proteins, polypeptides move through a universally conserved protein-conducting channel, formed by the Sec61/SecY complex that is located in the eukaryotic endoplasmic reticulum ...
    • Multiple mechanisms determine ER network morphology during the cell cycle in Xenopus egg extracts 

      Wang, Songyu; Romano, Fabian B.; Field, Christine M.; Mitchison, Tim J.; Rapoport, Tom A. (The Rockefeller University Press, 2013)
      In metazoans the endoplasmic reticulum (ER) changes during the cell cycle, with the nuclear envelope (NE) disassembling and reassembling during mitosis and the peripheral ER undergoing extensive remodeling. Here we address ...
    • A role for the two-helix finger of the SecA ATPase in protein translocation 

      Erlandson, Karl J.; Miller, Stephanie B. M.; Nam, Yunsun; Osborne, Andrew R.; Zimmer, Jochen; Rapoport, Tom A. (2015)
      An important step in the biosynthesis of many proteins is their partial or complete translocation across the plasma membrane in prokaryotes or the endoplasmic reticulum membrane in eukaryotes 1. In bacteria, secretory ...
    • The Signal Sequence Coding Region Promotes Nuclear Export of mRNA 

      Palazzo, Alexander F; Springer, Michael; Shibata, Yoko; Lee, Chung-Sheng; Dias, Anusha P; Rapoport, Tom A. (Public Library of Science, 2007)
      In eukaryotic cells, most mRNAs are exported from the nucleus by the transcription export (TREX) complex, which is loaded onto mRNAs after their splicing and capping. We have studied in mammalian cells the nuclear export ...
    • Structure of the SecY channel during initiation of protein translocation 

      Park, Eunyong; Ménétret, Jean-François; Gumbart, James C.; Ludtke, Steven J.; Li, Weikai; Whynot, Andrew; Rapoport, Tom A.; Akey, Christopher W. (2013)
      Many secretory proteins are targeted by signal sequences to a protein-conducting channel, formed by prokaryotic SecY- or eukaryotic Sec61-complexes, and are translocated across the membrane during their synthesis1,2. Crystal ...