Now showing items 1-2 of 2

    • CRISPR Guide RNA Design for Research Applications 

      Mohr, Stephanie; Hu, Yanhui; Ewen-Campen, Benjamin; Housden, Benjamin; Viswanatha, Raghuvir; Perrimon, Norbert (Wiley-Blackwell, 2016-09)
      The rapid rise of CRISPR as a technology for genome engineering and related research applications has created a need for algorithms and associated online tools that facilitate design of on‐target and effective guide RNAs ...
    • Pooled Genome-Wide CRISPR Screening for Basal and Context-Specific Fitness Gene Essentiality in Drosophila Cells 

      Viswanatha, Raghuvir; Li, Zhongchi; Hu, Yanhui; Perrimon, Norbert (eLife Sciences Publications, Ltd, 2018-07-27)
      Genome-wide screens in Drosophila cells have offered numerous insights into gene function, yet a major limitation has been the inability to stably deliver large multiplexed DNA libraries to cultured cells allowing barcoded ...