Now showing items 1-14 of 14

    • An Actin-Based Wave Generator Organizes Cell Motility 

      Weiner, Orion D; Marganski, William A; Wu, Lani F; Altschuler, Steven J; Kirschner, Marc W. (Public Library of Science, 2007)
      Although many of the regulators of actin assembly are known, we do not understand how these components act together to organize cell shape and movement. To address this question, we analyzed the spatial dynamics of a key ...
    • Bethesda Statement on Open Access Publishing 

      Brown, Patrick O.; Cabell, Diane; Chakravarti, Aravinda; Cohen, Barbara; Delamothe, Tony; Eisen, Michael; Grivell, Les; Guédon, Jean-Claude; Hawley, R. Scott; Johnson, Richard K.; Kirschner, Marc W.; Lipman, David; Lutzker, Arnold P.; Marincola, Elizabeth; Roberts, Richard J.; Rubin, Gerald M.; Schloegl, Robert; Siegel, Vivian; So, Anthony D.; Suber, Peter; Varmus, Harold E.; Velterop, Jan; Walport, Mark J.; Watson, Linda (2003)
    • The Cell Cycle and Differentiation in Stem Cells 

      Li, Victor Chun (2013-03-08)
      The relationship between cellular proliferation and differentiation is a major topic in cell biology. What we know comes from models of somatic cell differentiation, where it is widely viewed that cycling and differentiation ...
    • Direct observation of mammalian cell growth and size regulation 

      Son, Sungmin; Tzur, Amit; Weng, Yaochung; Jorgensen, Paul D; Kim, Jisoo; Kirschner, Marc W.; Manalis, Scott R. (2012)
      We introduce a microfluidic system for simultaneously measuring single cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 hours of growth data from mouse lymphoblast and ...
    • Dorsoventral Patterning in Hemichordates: Insights into Early Chordate Evolution 

      Lowe, Christopher J; Terasaki, Mark; Runft, Linda; Kwan, Kristen; Haigo, Saori; Aronowicz, Jochanan; Gruber, Chris; Gerhart, John; Wu, Michael; Freeman, Robert M; Lander, Eric Steven; Smith, Mark; Kirschner, Marc W. (Public Library of Science, 2006)
      We have compared the dorsoventral development of hemichordates and chordates to deduce the organization of their common ancestor, and hence to identify the evolutionary modifications of the chordate body axis after the ...
    • Emi1 preferentially inhibits ubiquitin chain elongation by the anaphase promoting complex 

      Wang, Weiping; Kirschner, Marc W. (2013)
      The anaphase promoting complex (APC) is the crucial ubiquitin ligase targeting the regulatory machinery of the cell cycle. Emi1, a major modulator of APC activity, is thought to act competitively as a pseudosubstrate. We ...
    • A General Lack of Compensation for Gene Dosage in Yeast 

      Springer, Michael; Weissman, Jonathan S; Kirschner, Marc W. (Nature Publishing Group, 2010)
      Gene copy number variation has been discovered in humans, between related species, and in different cancer tissues, but it is unclear how much of this genomic-level variation leads to changes in the level of protein ...
    • The Gonium pectorale genome demonstrates co-option of cell cycle regulation during the evolution of multicellularity 

      Hanschen, Erik R.; Marriage, Tara N.; Ferris, Patrick J.; Hamaji, Takashi; Toyoda, Atsushi; Fujiyama, Asao; Neme, Rafik; Noguchi, Hideki; Minakuchi, Yohei; Suzuki, Masahiro; Kawai-Toyooka, Hiroko; Smith, David R.; Sparks, Halle; Anderson, Jaden; Bakarić, Robert; Luria, Victor; Karger, Amir; Kirschner, Marc W.; Durand, Pierre M.; Michod, Richard E.; Nozaki, Hisayoshi; Olson, Bradley J. S. C. (Nature Publishing Group, 2016)
      The transition to multicellularity has occurred numerous times in all domains of life, yet its initial steps are poorly understood. The volvocine green algae are a tractable system for understanding the genetic basis of ...
    • Hem-1 Complexes Are Essential for Rac Activation, Actin Polymerization, and Myosin Regulation during Neutrophil Chemotaxis 

      Weiner, Orion D; Rentel, Maike C; Ott, Alex; Bourne, Henry R; Schliwa, Manfred; Brown, Glenn E.; Jedrychowski, Mark P.; Yaffe, Michael Bruce; Gygi, Steven P.; Cantley, Lewis C.; Kirschner, Marc W. (Public Library of Science, 2006)
      Migrating cells need to make different actin assemblies at the cell's leading and trailing edges and to maintain physical separation of signals for these assemblies. This asymmetric control of activities represents one ...
    • Hemichordate genomes and deuterostome origins 

      Simakov, Oleg; Kawashima, Takeshi; Marlétaz, Ferdinand; Jenkins, Jerry; Koyanagi, Ryo; Mitros, Therese; Hisata, Kanako; Bredeson, Jessen; Shoguchi, Eiichi; Gyoja, Fuki; Yue, Jia-Xing; Chen, Yi-Chih; Freeman, Robert M.; Sasaki, Akane; Hikosaka-Katayama, Tomoe; Sato, Atsuko; Fujie, Manabu; Baughman, Kenneth W.; Levine, Judith; Gonzalez, Paul; Cameron, Christopher; Fritzenwanker, Jens H.; Pani, Ariel M.; Goto, Hiroki; Kanda, Miyuki; Arakaki, Nana; Yamasaki, Shinichi; Qu, Jiaxin; Cree, Andrew; Ding, Yan; Dinh, Huyen H.; Dugan, Shannon; Holder, Michael; Jhangiani, Shalini N.; Kovar, Christie L.; Lee, Sandra L.; Lewis, Lora R.; Morton, Donna; Nazareth, Lynne V.; Okwuonu, Geoffrey; Santibanez, Jireh; Chen, Rui; Richards, Stephen; Muzny, Donna M.; Gillis, Andrew; Peshkin, Leonid; Wu, Michael; Humphreys, Tom; Su, Yi-Hsien; Putnam, Nicholas H.; Schmutz, Jeremy; Fujiyama, Asao; Yu, Jr-Kai; Tagawa, Kunifumi; Worley, Kim C.; Gibbs, Richard A.; Kirschner, Marc W.; Lowe, Christopher J.; Satoh, Noriyuki; Rokhsar, Daniel S.; Gerhart, John (2015)
      Acorn worms, also known as enteropneust (literally, ‘gut-breathing’) hemichordates, are marine invertebrates that share features with echinoderms and chordates. Together, these three phyla comprise the deuterostomes. Here ...
    • The master cell cycle regulator APC-Cdc20 regulates ciliary length and disassembly of the primary cilium 

      Wang, Weiping; Wu, Tao; Kirschner, Marc W (eLife Sciences Publications, Ltd, 2014)
      The primary cilium has an important role in signaling; defects in structure are associated with a variety of human diseases. Much of the most basic biology of this organelle is poorly understood, even basic mechanisms, ...
    • Microarray Discovery of New OGT Substrates: The Medulloblastoma Oncogene OTX2 Is O-GlcNAcylated 

      Ortiz-Meoz, Rodrigo F.; Merbl, Yifat; Kirschner, Marc W.; Walker, Suzanne (American Chemical Society, 2014)
      O-GlcNAc transferase (OGT) is a serine/threonine glycosyltransferase that is essential for development and continues to be critically important throughout life. Understanding OGT’s complex biology requires identifying its ...
    • Multiple Phases of Chondrocyte Enlargement Underlie Differences in Skeletal Proportions 

      Cooper, Kimberly L.; Oh, Seungeun; Sung, Yongjin; Dasari, Ramachandra R.; Kirschner, Marc W.; Tabin, Clifford J. (2013)
      Even a casual pass through the great halls of mammals in the world’s natural history museums highlights the wide diversity of skeletal proportions that allow us to distinguish between species even when reduced to their ...
    • Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis 

      Son, Sungmin; Kang, Joon Ho; Oh, Seungeun; Kirschner, Marc W.; Mitchison, T.J.; Manalis, Scott (The Rockefeller University Press, 2015)
      Osmotic regulation of intracellular water during mitosis is poorly understood because methods for monitoring relevant cellular physical properties with sufficient precision have been limited. Here we use a suspended ...