Now showing items 1-5 of 5

    • Deconstructing transcriptional heterogeneity in pluripotent stem cells 

      Kumar, Roshan M.; Cahan, Patrick; Shalek, Alex K.; Satija, Rahul; DaleyKeyser, AJay; Li, Hu; Zhang, Jin; Pardee, Keith; Gennert, David; Trombetta, John J.; Ferrante, Thomas C.; Regev, Aviv; Daley, George Q.; Collins, James J. (2014)
      SUMMARY Pluripotent stem cells (PSCs) are capable of dynamic interconversion between distinct substates, but the regulatory circuits specifying these states and enabling transitions between them are not well understood. ...
    • Fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissues 

      Lee, Je Hyuk; Daugharthy, Evan R; Scheiman, Jonathan; Kalhor, Reza; Ferrante, Thomas Charles; Terry, Richard; Turczyk, Brian M; Yang, Joyce L; Lee, Ho Suk; Aach, John Dennis; Zhang, Kun; Church, George McDonald (Springer Nature, 2015)
      RNA sequencing measures the quantitative change in gene expression over the whole transcriptome, but it lacks spatial context. On the other hand, in situ hybridization provides the location of gene expression, but only for ...
    • Highly Multiplexed Subcellular RNA Sequencing in Situ 

      Lee, Je Hyuk; Daugharthy, Evan; Scheiman, Jonathan; Kalhor, Reza; Ferrante, Thomas; Yang, Joyce; Terry, Richard; Jeanty, Sauveur; Li, Chao; Amamoto, Ryoji; Peters, Derek; Turczyk, Brian; Marblestone, Adam; Inverso, Samuel; Bernard, Amy; Mali, Prashant; Rios, Xavier; Aach, John; Church, George (American Association for the Advancement of Science, 2014-03-21)
      Understanding the spatial organization of gene expression with single nucleotide resolution requires localizing the sequences of expressed RNA transcripts within a cell in situ. Here we describe fluorescent in situ RNA ...
    • Photothermally triggered actuation of hybrid materials as a new platform for in vitro cell manipulation 

      Sutton, Amy Alexandra; Shirman, Tanya; Timonen, Jaakko; England, Grant Tyler; Kim, Philseok; Kolle, Mathias; Ferrante, Thomas Charles; Zarzar, Lauren; Strong, Elizabeth; Aizenberg, Joanna (Springer Nature, 2017)
      Mechanical forces in the cell’s natural environment have a crucial impact on growth, differentiation and behavior. Few areas of biology can be understood without taking into account how both individual cells and cell ...
    • Small airway-on-a-chip enables analysis of human lung inflammation and drug responses in vitro 

      Benam, Kambez H; Villenave, Remi; Lucchesi, Carolina; Varone, Antonio; Hubeau, Cedric; Lee, Hyun-Hee; Alves, Stephen E; Salmon, Michael; Ferrante, Thomas Charles; Weaver, James C.; Bahinski, Anthony; Hamilton, Geraldine A; Ingber, Donald Elliot (Nature Publishing Group, 2015)
      Here we describe development of a human ‘lung small airway-on-a-chip’ containing a differentiated, mucociliary, bronchiolar epithelium and an underlying microvascular endothelium that experiences fluid flow, which enables ...