Now showing items 1-6 of 6

    • 3-D Microwell Array System for Culturing Virus Infected Tumor Cells 

      El Assal, Rami; Gurkan, Umut A.; Chen, Pu; Juillard, Franceline; Tocchio, Alessandro; Chinnasamy, Thiruppathiraja; Beauchemin, Chantal; Unluisler, Sebnem; Canikyan, Serli; Holman, Alyssa; Srivatsa, Srikar; Kaye, Kenneth M.; Demirci, Utkan (Nature Publishing Group, 2016)
      Cancer cells have been increasingly grown in pharmaceutical research to understand tumorigenesis and develop new therapeutic drugs. Currently, cells are typically grown using two-dimensional (2-D) cell culture approaches, ...
    • Crystal Structure of the Gamma-2 Herpesvirus LANA DNA Binding Domain Identifies Charged Surface Residues Which Impact Viral Latency 

      Correia, Bruno; Cerqueira, Sofia A.; Beauchemin, Chantal; Pires de Miranda, Marta; Li, Shijun; Ponnusamy, Rajesh; Rodrigues, Lénia; Schneider, Thomas R.; Carrondo, Maria A.; Kaye, Kenneth M.; Simas, J. Pedro; McVey, Colin E. (Public Library of Science, 2013)
      Latency-associated nuclear antigen (LANA) mediates γ2-herpesvirus genome persistence and regulates transcription. We describe the crystal structure of the murine gammaherpesvirus-68 LANA C-terminal domain at 2.2 Å resolution. ...
    • Kaposi’s Sarcoma Herpesvirus Genome Persistence 

      Juillard, Franceline; Tan, Min; Li, Shijun; Kaye, Kenneth M. (Frontiers Media S.A., 2016)
      Kaposi’s sarcoma-associated herpesvirus (KSHV) has an etiologic role in Kaposi’s sarcoma, primary effusion lymphoma, and multicentric Castleman’s disease. These diseases are most common in immunocompromised individuals, ...
    • KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA 

      Ponnusamy, Rajesh; Petoukhov, Maxim V.; Correia, Bruno; Custodio, Tania F.; Juillard, Franceline; Tan, Min; Pires de Miranda, Marta; Carrondo, Maria A.; Simas, J. Pedro; Kaye, Kenneth M.; Svergun, Dmitri I.; McVey, Colin E. (Oxford University Press, 2015)
      Latency-associated nuclear antigen (LANA) is central to episomal tethering, replication and transcriptional regulation of γ2-herpesviruses. LANA binds cooperatively to the terminal repeat (TR) region of the viral episome ...
    • Printed Flexible Plastic Microchip for Viral Load Measurement through Quantitative Detection of Viruses in Plasma and Saliva 

      Shafiee, Hadi; Kanakasabapathy, Manoj Kumar; Juillard, Franceline; Keser, Mert; Sadasivam, Magesh; Yuksekkaya, Mehmet; Hanhauser, Emily; Henrich, Timothy J.; Kuritzkes, Daniel R.; Kaye, Kenneth M.; Demirci, Utkan (Nature Publishing Group, 2014)
      We report a biosensing platform for viral load measurement through electrical sensing of viruses on a flexible plastic microchip with printed electrodes. Point-of-care (POC) viral load measurement is of paramount importance ...
    • Stabilization of Myc through Heterotypic Poly-Ubiquitination by mLANA Is Critical for γ-Herpesvirus Lymphoproliferation 

      Rodrigues, Lénia; Popov, Nikita; Kaye, Kenneth M.; Simas, J. Pedro (Public Library of Science, 2013)
      Host colonization by lymphotropic γ-herpesviruses depends critically on expansion of viral genomes in germinal center (GC) B-cells. Myc is essential for the formation and maintenance of GCs. Yet, the role of Myc in the ...