Browsing by Author "Lesser, Cammie"
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Engineering Escherichia coli into a Protein Delivery System for Mammalian Cells
Reeves, Analise Z.; Spears, William E.; Du, Juan; Tan, Kah Yong; Wagers, Amy J.; Lesser, Cammie F. (American Chemical Society, 2015)Many Gram-negative pathogens encode type 3 secretion systems, sophisticated nanomachines that deliver proteins directly into the cytoplasm of mammalian cells. These systems present attractive opportunities for therapeutic ... -
A Functional Genomic Yeast Screen to Identify Pathogenic Bacterial Proteins
Slagowski, Naomi L; Kramer, Roger W; Morrison, Monica F; LaBaer, Joshua; Lesser, Cammie (Public Library of Science, 2008)Many bacterial pathogens promote infection and cause disease by directly injecting into host cells proteins that manipulate eukaryotic cellular processes. Identification of these translocated proteins is essential to ... -
GEF-H1 Mediated Control of NOD1 Dependent NF-κB Activation by Shigella Effectors
Fukazawa, Atsuko; Alonso, Carmen; Kurachi, Kiyotaka; Gupta, Sonal; Philpott, Dana J.; Lesser, Cammie; McCormick, Beth A.; Reinecker, Hans-Christian (Public Library of Science, 2008)Shigella flexneri has evolved the ability to modify host cell function with intracellular active effectors to overcome the intestinal barrier. The detection of these microbial effectors and the initiation of innate immune ... -
Identification and Characterization of a Candidate Wolbachia pipientis Type IV Effector That Interacts with the Actin Cytoskeleton
Sheehan, Kathy B.; Martin, MaryAnn; Lesser, Cammie F.; Isberg, Ralph R.; Newton, Irene L. G. (American Society for Microbiology, 2016)ABSTRACT Many bacteria live as intracellular symbionts, causing persistent infections within insects. One extraordinarily common infection is that of Wolbachia pipientis, which infects 40% of insect species and induces ... -
A Multifunctional Region of the Shigella Type 3 Effector IpgB1 Is Important for Secretion from Bacteria and Membrane Targeting in Eukaryotic Cells
Costa, Sonia C. P.; Lesser, Cammie F. (Public Library of Science, 2014)Type 3 secretion systems are complex nanomachines used by many Gram–negative bacteria to deliver tens of proteins (effectors) directly into host cells. Once delivered into host cells, effectors often target to specific ... -
A New Means To Identify Type 3 Secreted Effectors: Functionally Interchangeable Class IB Chaperones Recognize a Conserved Sequence
Costa, Sonia; Schmitz, Alexa M.; Jahufar, Fathima F.; Boyd, Justin D.; Cho, Min Y.; Glicksman, Marcie Ann; Lesser, Cammie (American Society of Microbiology, 2012)Many Gram-negative bacteria utilize specialized secretion systems to inject proteins (effectors) directly into host cells. Little is known regarding how bacteria ensure that only small subsets of the thousands of proteins ...