Now showing items 1-20 of 39

    • Age-associated NF-κB signaling in myofibers alters the satellite cell niche and re-strains muscle stem cell function 

      Oh, Juhyun; Sinha, Indranil; Tan, Kah Yong; Rosner, Bernard; Dreyfuss, Jonathan M.; Gjata, Ornela; Tran, Peter; Shoelson, Steven E.; Wagers, Amy J. (Impact Journals LLC, 2016)
      Skeletal muscle is a highly regenerative tissue, but muscle repair potential is increasingly compromised with advancing age. In this study, we demonstrate that increased NF-κB activity in aged muscle fibers contributes to ...
    • BCR-ABL enhances differentiation of long-term repopulating hematopoietic stem cells 

      Schemionek, M.; Elling, C.; Steidl, U.; Baumer, N.; Hamilton, A.; Spieker, T.; Gothert, J. R.; Stehling, M.; Wagers, Amy Jo; Huettner, Claudia Sonja; Tenen, Daniel Geoffrey; Tickenbrock, L.; Berdel, W. E.; Serve, H.; Holyoake, T. L.; Muller-Tidow, C.; Koschmieder, S. (American Society of Hematology, 2010)
      In a previously developed inducible transgenic mouse model of chronic myeloid leukemia, we now demonstrate that the disease is transplantable using BCR-ABL+ Lin−Sca-1+c-kit+ (LSK) cells. Interestingly, the phenotype is ...
    • Cell Type of Origin Influences the Molecular and Functional Properties of Mouse Induced Pluripotent Stem Cells 

      Polo, Jose M.; Liu, Susanna; Figueroa, Maria Eugenia; Kulalert, Warakorn; Eminli, Sarah; Tan, Kah Yong; Apostolou, Effie; Stadtfeld, Matthias; Li, Yushan; Shioda, Toshihiro; Natesan, Sridaran; Wagers, Amy Jo; Melnick, Ari; Evans, Todd; Hochedlinger, Konrad (Nature Publishing Group, 2010)
      Induced pluripotent stem cells (iPSCs) have been derived from various somatic cell populations through ectopic expression of defined factors. It remains unclear whether iPSCs generated from different cell types are molecularly ...
    • Cell-Cycle Dependent Expression of a Translocation-Mediated Fusion Oncogene Mediates Checkpoint Adaptation in Rhabdomyosarcoma 

      Kikuchi, Ken; Hettmer, Simone; Aslam, M. Imran; Michalek, Joel E.; Laub, Wolfram; Wilky, Breelyn A.; Loeb, David M.; Rubin, Brian P.; Wagers, Amy J.; Keller, Charles (Public Library of Science, 2014)
      Rhabdomyosarcoma is the most commonly occurring soft-tissue sarcoma in childhood. Most rhabdomyosarcoma falls into one of two biologically distinct subgroups represented by alveolar or embryonal histology. The alveolar ...
    • Circulating C3 is Necessary and Sufficient for Induction of Autoantibody-Mediated Arthritis in a Mouse Model 

      Monach, Paul A; Verschoor, Admar; Jacobs, Jonathan P; Carroll, Michael C.; Wagers, Amy Jo; Benoist, Christophe O.; Mathis, Diane J. (John Wiley & Sons, 2007)
      Objective. For the inflammation characteristic of rheumatoid arthritis, the relative contribution of mediators produced locally in the synovium versus those circulating systemically is unknown. Complement factor C3 is made ...
    • Cross-circulation and cell distribution kinetics in parabiotic mice 

      Gibney, Barry; Chamoto, Kenji; Lee, Grace S.; Simpson, Dinee C.; Miele, Lino F.; Tsuda, Akira; Konerding, Moritz A.; Wagers, Amy Jo; Mentzer, Steven J. (Wiley-Blackwell, 2011)
      Blood-borne nucleated cells participate not only in inflammation, but in tissue repair and regeneration. Because progenitor and stem cell populations have a low concentration in the blood, the circulation kinetics and ...
    • Defining markers and mechanisms of human somatic cell reprogramming 

      Ratanasirintrawoot, Sutheera (2014-02-25)
      Somatic cells can be reprogrammed into induced pluripotent stem (iPS) cells by over expression of the transcription factors OCT4, SOX2, KLF4 and c-MYC. Using serial live cell immunofluorescence imaging of human fibroblasts ...
    • Developmental regulation of myeloerythroid progenitor function by the Lin28b–let-7–Hmga2 axis 

      Rowe, R. Grant; Wang, Leo D.; Coma, Silvia; Han, Areum; Mathieu, Ronald; Pearson, Daniel S.; Ross, Samantha; Sousa, Patricia; Nguyen, Phi T.; Rodriguez, Antony; Wagers, Amy J.; Daley, George Q. (The Rockefeller University Press, 2016)
      For appropriate development, tissue and organ system morphogenesis and maturation must occur in synchrony with the overall developmental requirements of the host. Mistiming of such developmental events often results in ...
    • Diminished Schwann Cell Repair Responses Underlie Age-Associated Impaired Axonal Regeneration 

      Painter, Michio Wendell; Brosius Lutz, Amanda; Cheng, Yung-Chih; Latremoliere, Alban; Duong, Kelly; Miller, Christine; Posada, Sean; Cobos, Enrique J.; Zhang, Alan; Wagers, Amy Jo; Havton, Leif A.; Barres, Ben; Omura, Takao; Woolf, Clifford (Elsevier BV, 2014)
      The regenerative capacity of the peripheral nervous system declines with age. Why this occurs, however, is unknown. We demonstrate that 24-month-old mice exhibit an impairment of functional recovery after nerve injury ...
    • Distinct Malignant Behaviors of Mouse Myogenic Tumors Induced by Different Oncogenetic Lesions 

      Hettmer, Simone; Bronson, Roderick T.; Wagers, Amy J. (Frontiers Media S.A., 2015)
      Rhabdomyosarcomas (RMS) are heterogeneous cancers with myogenic differentiation features. The cytogenetic and mutational aberrations in RMS are diverse. This study examined differences in the malignant behavior of two ...
    • Early Life Nutrition Modulates Muscle Stem Cell Number: Implications for Muscle Mass and Repair 

      Woo, Melissa; Isganaitis, Elvira; Cerletti, Massimiliano; Fitzpatrick, Connor; Wagers, Amy Jo; Jimenez-Chillaron, Jose; Patti, Mary-Elizabeth (Mary Ann Liebert, 2011)
      Suboptimal nutrition during prenatal and early postnatal development is associated with increased risk for type 2 diabetes during adult life. A hallmark of such diabetes risk is altered body composition, including reduced ...
    • Engineering Escherichia coli into a Protein Delivery System for Mammalian Cells 

      Reeves, Analise Z.; Spears, William E.; Du, Juan; Tan, Kah Yong; Wagers, Amy J.; Lesser, Cammie F. (American Chemical Society, 2015)
      Many Gram-negative pathogens encode type 3 secretion systems, sophisticated nanomachines that deliver proteins directly into the cytoplasm of mammalian cells. These systems present attractive opportunities for therapeutic ...
    • Established cell surface markers efficiently isolate highly overlapping populations of skeletal muscle satellite cells by fluorescence-activated cell sorting 

      Maesner, Claire C.; Almada, Albert E.; Wagers, Amy J. (BioMed Central, 2016)
      Background: Fluorescent-activated cell sorting (FACS) has enabled the direct isolation of highly enriched skeletal muscle stem cell, or satellite cell, populations from postnatal tissue. Several distinct surface marker ...
    • FOXP3+ T Cells Recruited to Sites of Sterile Skeletal Muscle Injury Regulate the Fate of Satellite Cells and Guide Effective Tissue Regeneration 

      Castiglioni, Alessandra; Corna, Gianfranca; Rigamonti, Elena; Basso, Veronica; Vezzoli, Michela; Monno, Antonella; Almada, Albert E.; Mondino, Anna; Wagers, Amy J.; Manfredi, Angelo A.; Rovere-Querini, Patrizia (Public Library of Science, 2015)
      Muscle injury induces a classical inflammatory response in which cells of the innate immune system rapidly invade the tissue. Macrophages are prominently involved in this response and required for proper healing, as they ...
    • Functional genomic screening reveals asparagine dependence as a metabolic vulnerability in sarcoma 

      Hettmer, Simone; Schinzel, Anna C; Tchessalova, Daria; Schneider, Michaela; Parker, Christina L; Bronson, Roderick T; Richards, Nigel GJ; Hahn, William C; Wagers, Amy J (eLife Sciences Publications, Ltd, 2015)
      Current therapies for sarcomas are often inadequate. This study sought to identify actionable gene targets by selective targeting of the molecular networks that support sarcoma cell proliferation. Silencing of asparagine ...
    • Growth Differentiation Factor 11 Is a Circulating Factor that Reverses Age-Related Cardiac Hypertrophy 

      Loffredo, Francesco; Steinhauser, Matthew L.; Jay, Steven M.; Gannon, Joseph; Pancoast, James R.; Yalamanchi, Pratyusha; Sinha, Manisha; Dall’Osso, Claudia; Khong, Danika Mei Po; Shadrach, J; Miller, Christine; Singer, Britta S.; Stewart, Alex; Psychogios, Nikolaos; Gerszten, Robert Edgardo; Hartigan, Adam J.; Kim, Mi-Jeong; Serwold, Thomas Francis; Wagers, Amy Jo; Lee, Richard Theodore (Elsevier BV, 2013)
      The most common form of heart failure occurs with normal systolic function and often involves cardiac hypertrophy in the elderly. To clarify the biological mechanisms that drive cardiac hypertrophy in aging, we tested ...
    • The Hippo Transducer YAP1 Transforms Activated Satellite Cells and Is a Potent Effector of Embryonal Rhabdomyosarcoma Formation 

      Tremblay, Annie M.; Missiaglia, Edoardo; Galli, Giorgio G.; Hettmer, Simone; Urcia, Roby; Carrara, Matteo; Judson, Rebekah M; Thway, Khin; Nadal, Gema; Selfe, Joanna L.; Murray, Graeme; Calogero, Raffaele A.; De Bari, Cosimo; Zammit, Peter S.; Delorenzi, Mauro; Wagers, Amy Jo; Shipley, Janet; Wackerhage, Henning; Camargo, Fernando D. (Elsevier BV, 2014)
      The role of the Hippo pathway effector YAP1 in soft tissue sarcomas is poorly defined. Here we report that YAP1 activity is elevated in human embryonal rhabdomyosarcoma (ERMS). In mice, sustained YAP1 hyperactivity in ...
    • Id3 Is a Direct Transcriptional Target of Pax7 in Quiescent Satellite Cells 

      Kumar, Deepak; Shadrach, Jennifer; Wagers, Amy Jo; Lassar, Andrew B. (American Society for Cell Biology, 2009)
      Pax7 is a key regulator of skeletal muscle stem cells and is required along with Pax3 to generate skeletal muscle precursors. We have identified a collection of genes induced by either Pax3 or Pax7 in C2C12 muscle cells. ...
    • Identification of a myeloid committed progenitor as the cancer-initiating cell in acute promyelocytic leukemia 

      Guibal, F. C.; Alberich-Jorda, M.; Hirai, H.; Ebralidze, Alexander K.; Levantini, Elena; Di Ruscio, A.; Zhang, Pu; Santana-Lemos, B. A.; Neuberg, Donna S.; Wagers, Amy Jo; Rego, E. M.; Tenen, Daniel Geoffrey (American Society of Hematology, 2009)
      Acute promyelocytic leukemia (APL) is characterized by a block in differentiation and accumulation of promyelocytes in the bone marrow and blood. The majority of APL patients harbor the t(15:17) translocation leading to ...
    • Immuno-Analysis and FACS Sorting of Adult Muscle Fiber-Associated Stem/Precursor Cells 

      Conboy, Michael J.; Cerletti, Massimiliano; Wagers, Amy Jo; Conboy, Irina M. (Springer Science + Business Media, 2010)
      We describe the immunostaining methods we commonly use to detect the more robust cell markers identifying the various cell populations obtained by the enzymatic and or mechanical dissociation of muscle satellite cells: ...