Browsing by Author "Mekalanos, John"
Now showing items 1-15 of 15
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Characterization of the Antibacterial Activity of the Type VI Secretion System
Ho, Brian Thomas (2014-02-25)This dissertation summarizes advances made toward understanding of the composition, structure, mechanism, and regulation of the bacterial type VI secretion system (T6SS). The T6SS is a widely conserved bacterial nanomachine ... -
Characterization of the RpoN Regulon Reveals Differential Regulation of T6SS and New Flagellar Operons in Vibrio cholerae O37 Strain V52
Dong, Tao; Mekalanos, John J. (Oxford University Press, 2012)The alternative sigma factor RpoN is an essential colonization factor of Vibrio cholerae and controls important cellular functions including motility and type VI secretion (T6SS). The RpoN regulon has yet to be clearly ... -
Cholera Toxin Promotes Pathogen Acquisition of Host-Derived Nutrients
Rivera-Chávez, Fabian; Mekalanos, John (Springer Science and Business Media LLC, 2019-07-31)Vibrio cholerae is the causative agent of cholera, a potentially lethal enteric bacterial infection1. Cholera toxin (CT) is required for V. cholerae to cause severe disease and is also thought to promote transmission of ... -
DNA Damage and Reactive Nitrogen Species are Barriers to Vibrio cholerae Colonization of the Infant Mouse Intestine
Davies, Bryan William; Bogard, Ryan; Dupes, Nicole M.; Gerstenfeld, Tyler A. I.; Simmons, Lyle A.; Mekalanos, John J. (Public Library of Science, 2011)Ingested Vibrio cholerae pass through the stomach and colonize the small intestines of its host. Here, we show that V. cholerae requires at least two types of DNA repair systems to efficiently compete for colonization of ... -
Functional Analysis of VopF Activity Required for Colonization in Vibrio cholerae
Tam, Vincent C.; Suzuki, Masato; Coughlin, Margaret; Saslowsky, David; Biswas, Kuntal; Lencer, Wayne Isaac; Faruque, Shah M.; Mekalanos, John J. (American Society of Microbiology, 2010)Vibrio cholerae, a Gram-negative facultative pathogen, is the etiologic agent for the diarrheal disease cholera. We previously characterized a clinical isolate, AM-19226, that translocates a type III secretion system (T3SS) ... -
The Highly Conserved Bacterial RNase YbeY Is Essential in Vibrio cholerae, Playing a Critical Role in Virulence, Stress Regulation, and RNA Processing
Vercruysse, Maarten; Köhrer, Caroline; Davies, Bryan W.; Arnold, Markus F. F.; Mekalanos, John J.; RajBhandary, Uttam L.; Walker, Graham C. (Public Library of Science, 2014)YbeY, a highly conserved protein, is an RNase in E. coli and plays key roles in both processing of the critical 3′ end of 16 S rRNA and in 70 S ribosome quality control under stress. These central roles account for YbeY's ... -
MetR-Regulated Vibrio cholerae Metabolism Is Required for Virulence
Bogard, Ryan William; Davies, Bryan William; Mekalanos, John J. (American Society of Microbiology, 2012)LysR-type transcriptional regulators (LTTRs) are the largest, most diverse family of prokaryotic transcription factors, with regulatory roles spanning metabolism, cell growth and division, and pathogenesis. Using a ... -
Microbiota-Targeted Maternal Antibodies Protect Neonates From Enteric Infection
Zheng, Wen; Zhao, Wenjing; Wu, Meng; Song, Xinyang; Caro, Florence; Sun, Ximei; Gazzaniga, Francesca; Stefanetti, Giuseppe; Oh, Sungwhan; Mekalanos, John; Kasper, Dennis (Springer Science and Business Media LLC, 2020-01-23)Although maternal antibodies protect newborns from infection, little is known about how protective antibodies are induced without prior pathogen exposure. Here we show that neonatal mice lacking the capacity to produce IgG ... -
PAAR-repeat proteins sharpen and diversify the Type VI secretion system spike
Shneider, Mikhail M.; Buth, Sergey A.; Ho, Brian T.; Basler, Marek; Mekalanos, John J.; Leiman, Petr G. (2013)The bacterial type VI secretion system (T6SS) is a large multi-component, dynamic macromolecular machine that plays an important role in the ecology of many Gram negative bacteria. T6SS is responsible for translocation of ... -
Phase Variable O Antigen Biosynthetic Genes Control Expression of the Major Protective Antigen and Bacteriophage Receptor in Vibrio cholerae O1
Seed, Kimberley D.; Faruque, Shah M.; Mekalanos, John J.; Calderwood, Stephen Beaven; Qadri, Firdausi; Camilli, Andrew (Public Library of Science, 2012)The Vibrio cholerae lipopolysaccharide O1 antigen is a major target of bacteriophages and the human immune system and is of critical importance for vaccine design. We used an O1-specific lytic bacteriophage as a tool to ... -
Quorum Regulated Resistance of Vibrio cholerae against Environmental Bacteriophages
Hoque, M. Mozammel; Naser, Iftekhar Bin; Bari, S. M. Nayeemul; Zhu, Jun; Mekalanos, John J.; Faruque, Shah M. (Nature Publishing Group, 2016)Predation by bacteriophages can significantly influence the population structure of bacterial communities. Vibrio cholerae the causative agent of cholera epidemics interacts with numerous phages in the aquatic ecosystem, ... -
Secretome Analysis of Vibrio cholerae Type VI Secretion System Reveals a New Effector-Immunity Pair
Altindis, Emrah; Dong, Tao; Catalano, Christy; Mekalanos, John (American Society of Microbiology, 2015)ABSTRACT The type VI secretion system (T6SS) is a dynamic macromolecular organelle that many Gram-negative bacteria use to inhibit or kill other prokaryotic or eukaryotic cells. The toxic effectors of T6SS are delivered ... -
Sigma E Regulators Control Hemolytic Activity and Virulence in a Shrimp Pathogenic Vibrio harveyi
Rattanama, Pimonsri; Thompson, Janelle R.; Kongkerd, Natthawan; Srinitiwarawong, Kanchana; Vuddhakul, Varaporn; Mekalanos, John J. (Public Library of Science, 2012)Members of the genus Vibrio are important marine and aquaculture pathogens. Hemolytic activity has been identified as a virulence factor in many pathogenic vibrios including V. cholerae, V. parahaemolyticus, V. alginolyticus, ... -
Vibrio Cholerae Proteome-Wide Screen for Immunostimulatory Proteins Identifies Phosphatidylserine Decarboxylase as a Novel Toll-Like Receptor 4 Agonist
Thanawastien, Ann; Montor, Wagner R.; LaBaer, Joshua; Mekalanos, John J.; Yoon, Sanghyun James (Public Library of Science, 2009)Recognition of conserved bacterial components provides immediate and efficient immune responses and plays a critical role in triggering antigen-specific adaptive immunity. To date, most microbial components that are detected ... -
Vibrio cholerae type 6 secretion system effector trafficking in target bacterial cells
Ho, Brian; Fu, Yang; Dong, Tao; Mekalanos, John (Proceedings of the National Academy of Sciences, 2017-08-14)This work demonstrates that it is possible to alter the prey target range of the antibacterial activity of the type 6 secretion system (T6SS). Being able to change the T6SS target specificity is the first step toward using ...