Now showing items 1-12 of 12

    • Absolute quantification of somatic DNA alterations in human cancer 

      Carter, Scott L.; Cibulskis, Kristian; Helman, Elena; McKenna, Aaron; Shen, Hui; Zack, Travis; Laird, Peter W.; Onofrio, Robert C.; Winckler, Wendy; Weir, Barbara A.; Beroukhim, Rameen; Pellman, David; Levine, Douglas A.; Lander, Eric S.; Meyerson, Matthew; Getz, Gad (2015)
      We developed a computational method (ABSOLUTE) that infers tumor purity and malignant cell ploidy directly from analysis of somatic DNA alterations. ABSOLUTE can detect subclonal heterogeneity, somatic homozygosity, and ...
    • CHROMOTHRIPSIS FROM DNA DAMAGE IN MICRONUCLEI 

      Zhang, Cheng-Zhong; Spektor, Alexander; Cornils, Hauke; Francis, Joshua M.; Jackson, Emily K.; Liu, Shiwei; Meyerson, Matthew; Pellman, David (2015)
      Genome sequencing has uncovered a new mutational phenomenon in cancer and congenital disorders called chromothripsis. Chromothripsis is characterized by extensive genomic rearrangements and an oscillating pattern of DNA ...
    • The Hippo Pathway Effector YAP Regulates Cytokinesis 

      Bui, Duyen Amy (2015-05-17)
      Yes-associated protein (YAP) is a co-transcription factor that acts downstream of the evolutionarily conserved Hippo pathway. Canonically, this pathway regulates tissue growth in flies and mammals, by controlling the nuclear ...
    • HURP Permits MTOC Sorting for Robust Meiotic Spindle Bipolarity, Similar to Extra Centrosome Clustering in Cancer Cells 

      Breuer, Manuel; Kolano, Agnieszka; Kwon, Mijung; Li, Chao-Chin; Tsai, Ting-Fen; Pellman, David Steven; Brunet, Stephane; Verlhac, Marie-Helene (Rockefeller University Press, 2010)
      In contrast to somatic cells, formation of acentriolar meiotic spindles relies on the organization of microtubules (MTs) and MT-organizing centers (MTOCs) into a stable bipolar structure. The underlying mechanisms are still ...
    • Inhibition of Cdc42 during mitotic exit is required for cytokinesis 

      Atkins, Benjamin D.; Yoshida, Satoshi; Saito, Koji; Wu, Chi-Fang; Lew, Daniel J.; Pellman, David (The Rockefeller University Press, 2013)
      The role of Cdc42 and its regulation during cytokinesis is not well understood. Using biochemical and imaging approaches in budding yeast, we demonstrate that Cdc42 activation peaks during the G1/S transition and during ...
    • Inhibition of Cdc42 during mitotic exit is required for cytokinesis in Saccharomyces cerevisiae 

      Atkins, Benjamin David (2014-02-25)
      Rho GTPases are highly conserved regulators of cell polarity and the actin cytoskeleton. The role of the Rho GTPase Cdc42 and its regulation during cell division is not well understood. Using biochemical and imaging ...
    • Linking abnormal mitosis to the acquisition of DNA damage 

      Ganem, Neil J.; Pellman, David (The Rockefeller University Press, 2012)
      Cellular defects that impair the fidelity of mitosis promote chromosome missegregation and aneuploidy. Increasing evidence reveals that errors in mitosis can also promote the direct and indirect acquisition of DNA damage ...
    • Microtubule sliding activity of a kinesin-8 promotes spindle assembly and spindle length control 

      Su, Xiaolei; Arellano-Santoyo, Hugo; Portran, Didier; Gaillard, Jeremie; Vantard, Marylin; Thery, Manuel; Pellman, David (2013)
      Molecular motors play critical roles in the formation of mitotic spindles, either through controlling the stability of individual microtubules, or by cross-linking and sliding microtubule arrays. Kinesin-8 motors are best ...
    • Modeling the Initiation of Ewing Sarcoma Tumorigenesis in Differentiating Human Embryonic Stem Cells 

      Gordon, David J.; Motwani, Mona; Pellman, David (2015)
      Oncogenic transformation in Ewing sarcoma tumors is driven by the fusion oncogene EWS-FLI1. However, despite the well-established role of EWS-FLI1 in tumor initiation, the development of models of Ewing sarcoma in human ...
    • Polyploidy can drive rapid adaptation in yeast 

      Selmecki, Anna; Maruvka, Yosef E.; Richmond, Phillip A.; Guillet, Marie; Shoresh, Noam; Sorenson, Amber; De, Subhajyoti; Kishony, Roy; Michor, Franziska; Dowell, Robin; Pellman, David (2015)
      Polyploidy is observed across the tree of life, yet its influence on evolution remains incompletely understood1–4. Polyploidy, usually whole genome duplication (WGD), is proposed to alter the rate of evolutionary adaptation. ...
    • Regulation of Microtubule Dynamics by Molecular Motors 

      Su, Xiaolei (2013-02-26)
      Kinesin superfamily motors have a well-characterized ability to move along microtubules and transport cargo. However, some members of the kinesin superfamily can also remodel microtubule networks by controlling tubulin ...
    • Triplication of a 21q22 region contributes to B cell transformation through HMGN1 overexpression and loss of histone H3 lysine 27 trimethylation 

      Lane, Andrew A.; Chapuy, Bjoern; Lin, Charles Y.; Tivey, Trevor; Li, Hubo; Townsend, Elizabeth C.; van Bodegom, Diederik; Day, Tovah A.; Wu, Shuo-Chieh; Liu, Huiyun; Yoda, Akinori; Alexe, Gabriela; Schinzel, Anna C.; Sullivan, Timothy J.; Malinge, Sébastien; Taylor, Jordan E.; Stegmaier, Kimberly; Jaffe, Jacob D.; Bustin, Michael; te Kronnie, Geertruy; Izraeli, Shai; Harris, Marian; Stevenson, Kristen E.; Neuberg, Donna; Silverman, Lewis B.; Sallan, Stephen E.; Bradner, James E.; Hahn, William C.; Crispino, John D.; Pellman, David; Weinstock, David M. (2014)
      Down syndrome confers a 20-fold increased risk of B cell acute lymphoblastic leukemia (B-ALL)1 and polysomy 21 is the most frequent somatic aneuploidy amongst all B-ALLs2. Yet, the mechanistic links between chr.21 triplication ...