Now showing items 1-6 of 6

    • In vivo cell cycle profiling in xenograft tumors by quantitative intravital microscopy 

      Chittajallu, Deepak R; Florian, Stefan; Kohler, Rainer H; Iwamoto, Yoshiko; Orth, James D; Weissleder, Ralph; Danuser, Gaudenz; Mitchison, Timothy J (2015)
      Quantification of cell-cycle state at a single-cell level is essential to understand fundamental three-dimensional biological processes such as tissue development and cancer. Analysis of 3D in vivo images, however, is very ...
    • IRF3 and type I interferons fuel a fatal response to myocardial infarction 

      King, Kevin R; Aguirre, Aaron Dominic; Ye, Yu-Xiang; Sun, Yuan; Roh, Jason David; Ng, Richard Patrick; Kohler, Rainer H.; Arlauckas, Sean Philip; Iwamoto, Yoshiko; Savol, Andrej J; Sadreyev, Ruslan; Kelly, Mark; Fitzgibbons, Timothy P; Fitzgerald, Katherine A; Mitchison, Timothy J.; Libby, Peter; Nahrendorf, Matthias; Weissleder, Ralph (Springer Nature, 2017)
      Interferon regulatory factor 3 (IRF3) and type I interferons (IFNs) protect against infections and cancer, but excessive IRF3 activation and type I IFN production cause autoinflammatory conditions such as Aicardi–Goutières ...
    • Live Imaging of Cysteine-Cathepsin Activity Reveals Dynamics of Focal Inflammation, Angiogenesis, and Polyp Growth 

      Gounaris, Elias; Tung, Ching H.; Restaino, Clifford; Maehr, Rene; Kohler, Rainer H.; Joyce, Johanna A.; Plough, Hidde L.; Barrett, Terrence A.; Weissleder, Ralph; Khazaie, Khashayarsha (Public Library of Science, 2008)
      It has been estimated that up to 30% of detectable polyps in patients regress spontaneously. One major challenge in the evaluation of effective therapy of cancer is the readout for tumor regression and favorable biological ...
    • Single cell imaging of Bruton's Tyrosine Kinase using an irreversible inhibitor 

      Turetsky, Anna; Kim, Eunha; Kohler, Rainer H.; Miller, Miles A.; Weissleder, Ralph (Nature Publishing Group, 2014)
      A number of Bruton's tyrosine kinase (BTK) inhibitors are currently in development, yet it has been difficult to visualize BTK expression and pharmacological inhibition in vivo in real time. We synthesized a fluorescent, ...
    • Single cell resolution in vivo imaging of DNA damage following PARP inhibition 

      Yang, Katherine S.; Kohler, Rainer H.; Landon, Matthieu; Giedt, Randy; Weissleder, Ralph (Nature Publishing Group, 2015)
      Targeting DNA repair pathways is a powerful strategy to treat cancers. To gauge efficacy in vivo, typical response markers include late stage effects such as tumor shrinkage, progression free survival, or invasive repeat ...
    • Tumor associated macrophages act as a slow-release reservoir of nano-therapeutic Pt(IV) pro-drug 

      Miller, Miles A.; Zheng, Yao-Rong; Gadde, Suresh; Pfirschke, Christina; Zope, Harshal; Engblom, Camilla; Kohler, Rainer H.; Iwamoto, Yoshiko; Yang, Katherine S.; Askevold, Bjorn; Kolishetti, Nagesh; Pittet, Mikael; Lippard, Stephen J.; Farokhzad, Omid C.; Weissleder, Ralph (2015)
      Therapeutic nanoparticles (TNPs) aim to deliver drugs more safely and effectively to cancers, yet clinical results have been unpredictable owing to limited in vivo understanding. Here we use single-cell imaging of intratumoral ...