Browsing by Author "Coloff, Jonathan L."
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Combining Amine Metabolomics and Quantitative Proteomics of Cancer Cells Using Derivatization with Isobaric Tags
Murphy, J. Patrick; Everley, Robert A.; Coloff, Jonathan L.; Gygi, Steven P. (American Chemical Society, 2014)Quantitative metabolomics and proteomics technologies are powerful approaches to explore cellular metabolic regulation. Unfortunately, combining the two technologies typically requires different LC-MS setups for sensitive ... -
Degradation of HK2 by chaperone-mediated autophagy promotes metabolic catastrophe and cell death
Xia, Hong-guang; Najafov, Ayaz; Geng, Jiefei; Galan-Acosta, Lorena; Han, Xuemei; Guo, Yuan; Shan, Bing; Zhang, Yaoyang; Norberg, Erik; Zhang, Tao; Pan, Lifeng; Liu, Junli; Coloff, Jonathan L.; Ofengeim, Dimitry; Zhu, Hong; Wu, Kejia; Cai, Yu; Yates, John R.; Zhu, Zhengjiang; Yuan, Junying; Vakifahmetoglu-Norberg, Helin (The Rockefeller University Press, 2015)Hexokinase II (HK2), a key enzyme involved in glucose metabolism, is regulated by growth factor signaling and is required for initiation and maintenance of tumors. Here we show that metabolic stress triggered by perturbation ... -
HIF1α and HIF2α Exert Distinct Nutrient Preferences in Renal Cells
Arreola, Alexandra; Cowey, C. Lance; Coloff, Jonathan L.; Rathmell, Jeffrey C.; Rathmell, W. Kimryn (Public Library of Science, 2014)Background: Hypoxia Inducible Factors (HIF1α and HIF2α) are commonly stabilized and play key roles related to cell growth and metabolic programming in clear cell renal cell carcinoma. The relationship of these factors to ... -
Starved epithelial cells uptake extracellular matrix for survival
Muranen, Taru; Iwanicki, Marcin P.; Curry, Natasha L.; Hwang, Julie; DuBois, Cory D.; Coloff, Jonathan L.; Hitchcock, Daniel S.; Clish, Clary B.; Brugge, Joan S.; Kalaany, Nada Y. (Nature Publishing Group, 2017)Extracellular matrix adhesion is required for normal epithelial cell survival, nutrient uptake and metabolism. This requirement can be overcome by oncogene activation. Interestingly, inhibition of PI3K/mTOR leads to apoptosis ...