Now showing items 1-18 of 18

    • 3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of \(^{18}O\)-Trehalose in Mouse Sperm Nucleus 

      Lechene, Claude P.; Lee, Gloria Y.; Poczatek, J. Collin; Toner, Mehmet; Biggers, John Dennis (Public Library of Science, 2012)
      The prevalence of genetically engineered mice in medical research has led to ever increasing storage costs. Trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following ...
    • Brain Tumor Cells in Circulation Are Enriched for Mesenchymal Gene Expression 

      Sullivan, James P; Nahed, Brian Vala; Madden, M. W.; Oliveira, S. M.; Springer, S.; Bhere, Deepak; Chi, A. S.; Wakimoto, Hiroaki; Rothenberg, S. M.; Sequist, Lecia VanDam; Kapur, R.; Shah, Khalid A.; Iafrate, Anthony John; Curry, William Thomas; Loeffler, Jay Steven; Batchelor, Tracy Todd; Louis, David N.; Toner, Mehmet; Maheswaran, Shyamala; Haber, Daniel Arie (American Association for Cancer Research (AACR), 2014)
      Glioblastoma (GBM) is a highly aggressive brain cancer characterized by local invasion and angiogenic recruitment, yet metastatic dissemination is extremely rare. Here, we adapted a microfluidic device to deplete hematopoietic ...
    • Burn Injury Reduces Neutrophil Directional Migration Speed in Microfluidic Devices 

      Butler, Kathryn Lee; Ambravaneswaran, Vijayakrishnan; Agrawal, Nitin; Bilodeau, Maryelizabeth; Toner, Mehmet; Tompkins, Ronald Gary; Fagan, Shawn P.; Irimia, Daniel (Public Library of Science, 2010)
      Thermal injury triggers a fulminant inflammatory cascade that heralds shock, end-organ failure, and ultimately sepsis and death. Emerging evidence points to a critical role for the innate immune system, and several studies ...
    • Collective and Individual Migration following the Epithelial-Mesenchymal Transition 

      Wong, Ian Y.; Javaid, Sarah; Wong, Elisabeth A.; Perk, Sinem; Haber, Daniel A.; Toner, Mehmet; Irimia, Daniel (2014)
      During cancer progression, malignant cells in the tumour invade surrounding tissues. This transformation of adherent cells to a motile phenotype has been associated with the epithelial mesenchymal transition (EMT). Here, ...
    • Continuous Flow Microfluidic Bioparticle Concentrator 

      Martel, Joseph M.; Smith, Kyle C.; Dlamini, Mcolisi; Pletcher, Kendall; Yang, Jennifer; Karabacak, Murat; Haber, Daniel A.; Kapur, Ravi; Toner, Mehmet (Nature Publishing Group, 2015)
      Innovative microfluidic technology has enabled massively parallelized and extremely efficient biological and clinical assays. Many biological applications developed and executed with traditional bulk processing techniques ...
    • Controlled induction of distributed microdeformation in wounded tissue via a microchamber array dressing 

      Kane, Bartholomew J.; Younan, George; Helm, Douglas; Dastouri, Pouya; Prentice-Mott, Harrison; Irimia, Daniel; Chan, Rodney K.; Toner, Mehmet; Orgill, Dennis Paul (Wiley-Blackwell, 2010)
      Mechanical stimuli are known to play an important role in determining the structure and function of living cells and tissues. Recent studies have highlighted the role of mechanical signals in mammalian dermal wound healing. ...
    • Deformability of Tumor Cells versus Blood Cells 

      Shaw Bagnall, Josephine; Byun, Sangwon; Begum, Shahinoor; Miyamoto, David T.; Hecht, Vivian C.; Maheswaran, Shyamala; Stott, Shannon L.; Toner, Mehmet; Hynes, Richard O.; Manalis, Scott R. (Nature Publishing Group, 2015)
      The potential for circulating tumor cells (CTCs) to elucidate the process of cancer metastasis and inform clinical decision-making has made their isolation of great importance. However, CTCs are rare in the blood, and ...
    • Engineered Trehalose Permeable to Mammalian Cells 

      Abazari, Alireza; Meimetis, Labros G.; Budin, Ghyslain; Bale, Shyam Sundhar; Weissleder, Ralph; Toner, Mehmet (Public Library of Science, 2015)
      Trehalose is a naturally occurring disaccharide which is associated with extraordinary stress-tolerance capacity in certain species of unicellular and multicellular organisms. In mammalian cells, presence of intra- and ...
    • Integration of Architectural and Cytologic Driven Image Algorithms for Prostate Adenocarcinoma Identification 

      Hipp, Jason; Monaco, James; Kunju, L. Priya; Cheng, Jerome; Yagi, Yukako; Rodriguez-Canales, Jaime; Emmert-Buck, Michael R.; Hewitt, Stephen; Feldman, Michael D.; Tomaszewski, John E.; Toner, Mehmet; Tompkins, Ronald G.; Flotte, Thomas; Lucas, David; Gilbertson, John R.; Madabhushi, Anant; Balis, Ulysses (IOS Press, 2012)
      Introduction:: The advent of digital slides offers new opportunities within the practice of pathology such as the use of image analysis techniques to facilitate computer aided diagnosis (CAD) solutions. Use of CAD holds ...
    • Live Pups from Evaporatively Dried Mouse Sperm Stored at Ambient Temperature for up to 2 Years 

      Liu, Jie; Lee, Gloria Y.; Lawitts, Joel A.; Toner, Mehmet; Biggers, John D. (Public Library of Science, 2014)
      The purpose of this study is to develop a mouse sperm preservation method based on evaporative drying. Mouse sperm were evaporatively dried and stored at 4°C and ambient temperature for 3 months to 2 years. Upon rehydration, ...
    • A microfluidic device for label-free, physical capture of circulating tumor cell-clusters 

      Sarioglu, A. Fatih; Aceto, Nicola; Kojic, Nikola; Donaldson, Maria C.; Zeinali, Mahnaz; Hamza, Bashar; Engstrom, Amanda; Zhu, Huili; Sundaresan, Tilak K.; Miyamoto, David T.; Luo, Xi; Bardia, Aditya; Wittner, Ben S.; Ramaswamy, Sridhar; Shioda, Toshi; Ting, David T.; Stott, Shannon L.; Kapur, Ravi; Maheswaran, Shyamala; Haber, Daniel A.; Toner, Mehmet (2015)
      Cancer cells metastasize through the bloodstream either as single migratory circulating tumor cells (CTCs) or as multicellular groupings (CTC-clusters). Existing technologies for CTC enrichment are designed primarily to ...
    • Particle Focusing in Microchannels 

      Martel, Joseph Maurice (2014-02-25)
      The ability to control the motion of particles and cells in microchannels has been a center of fascination since the advent of microfluidics. Entire fields have been created in order to accomplish separation, volume reduction ...
    • Preservation of Mouse Sperm by Convective Drying and Storing in 3-O-Methyl-D-Glucose 

      Liu, Jie; Lee, Gloria Y.; Lawitts, Joel A.; Toner, Mehmet; Biggers, John Dennis (Public Library of Science, 2012)
      With the fast advancement in the genetics and bio-medical fields, the vast number of valuable transgenic and rare genetic mouse models need to be preserved. Preservation of mouse sperm by convective drying and subsequent ...
    • Resolving Cancer-Stroma Interfacial Signaling and Interventions with Micropatterned Tumor-Stromal Assays 

      Shen, Keyue; Luk, Samantha; Hicks, Daniel F; Elman, Jessica S; Bohr, Stefan; Iwamoto, Yoshiko; Murray, Ryan; Pena, Kristen; Wang, Fangjing; Seker, Erkin; Weissleder, Ralph; Yarmush, Martin L; Toner, Mehmet; Sgroi, Dennis; Parekkadan, Biju (2014)
      Tumor-stromal interactions are a determining factor in cancer progression. In vivo, the interaction interface is associated with spatially-resolved distributions of cancer and stromal phenotypes. Here, we establish a ...
    • RNA Sequencing of Pancreatic Circulating Tumour Cells Implicates WNT Signaling in Metastasis 

      Yu, Min; Ting, David Tsai; Stott, Shannon; Wittner, Ben; Ozsolak, Fatih; Paul, Suchismita; Ciciliano, Jordan C.; Smas, Malgorzata E.; Winokur, Daniel; Gilman, Anna J.; Ulman, Matthew J.; Xega, Kristina; Contino, Gianmarco; Alagesan, Brinda; Brannigan, Brian W.; Milos, Patrice M.; Ryan, David P.; Sequist, Lecia VanDam; Bardeesy, Nabeel; Ramaswamy, Sridhar; Toner, Mehmet; Maheswaran, Shyamala; Haber, Daniel Arie (2012)
      Circulating tumour cells (CTCs) shed into blood from primary cancers include putative precursors that initiate distal metastases. While these cells are extraordinarily rare, they may identify cellular pathways contributing ...
    • The Role of Physical Stabilization in Whole Blood Preservation 

      Wong, Keith H. K.; Sandlin, Rebecca D.; Carey, Thomas R.; Miller, Kathleen L.; Shank, Aaron T.; Oklu, Rahmi; Maheswaran, Shyamala; Haber, Daniel A.; Irimia, Daniel; Stott, Shannon L.; Toner, Mehmet (Nature Publishing Group, 2016)
      The rapid degradation of blood ex vivo imposes logistical limitations on the utilization of blood-borne cells in medical diagnostics and scientific investigations. A fundamental but overlooked aspect in the storage of this ...
    • Single-Cell RNA Sequencing Identifies Extracellular Matrix Gene Expression by Pancreatic Circulating Tumor Cells 

      Ting, David T.; Wittner, Ben S.; Ligorio, Matteo; Jordan, Nicole Vincent; Shah, Ajay M.; Miyamoto, David T.; Aceto, Nicola; Bersani, Francesca; Brannigan, Brian W.; Xega, Kristina; Ciciliano, Jordan C.; Zhu, Huili; MacKenzie, Olivia C.; Trautwein, Julie; Arora, Kshitij S.; Shahid, Mohammad; Ellis, Haley L.; Qu, Na; Bardeesy, Nabeel; Rivera, Miguel N.; Deshpande, Vikram; Ferrone, Cristina R.; Kapur, Ravi; Ramaswamy, Sridhar; Shioda, Toshi; Toner, Mehmet; Maheswaran, Shyamala; Haber, Daniel A. (2014)
      SUMMARY Circulating tumor cells (CTCs) are shed from primary tumors into the bloodstream, mediating the hematogenous spread of cancer to distant organs. To define their composition, we compared genome-wide expression ...
    • Successful Supercooled Liver Storage for 4 Days 

      Berendsen, Tim A.; Bruinsma, Bote G.; Puts, Catheleyne F.; Saeidi, Nima; Usta, O. Berk; Uygun, Basak E.; Izamis, Maria-Louisa; Toner, Mehmet; Yarmush, Martin L.; Uygun, Korkut (2014)
      The realization of long–term human organ preservation will have groundbreaking effects on the current practice of transplantation. Herein we present a novel technique based on sub–zero non–freezing tissue preservation and ...