Now showing items 1-20 of 20

    • BEAMing and Droplet Digital PCR Analysis of Mutant IDH1 mRNA in Glioma Patient Serum and Cerebrospinal Fluid Extracellular Vesicles 

      Chen, Walter W; Balaj, Leonora; Liau, Linda M; Samuels, Michael L; Kotsopoulos, Steve K; Maguire, Casey A; LoGuidice, Lori; Soto, Horacio; Garrett, Matthew; Zhu, Lin Dan; Sivaraman, Sarada; Chen, Clark; Wong, Eric T; Carter, Bob S; Hochberg, Fred H; Breakefield, Xandra O; Skog, Johan (Nature Publishing Group, 2013)
      Development of biofluid-based molecular diagnostic tests for cancer is an important step towards tumor characterization and real-time monitoring in a minimally invasive fashion. Extracellular vesicles (EVs) are released ...
    • Chip-based analysis of exosomal mRNA mediating drug resistance in glioblastoma 

      Shao, Huilin; Chung, Jaehoon; Lee, Kyungheon; Balaj, Leonora; Min, Changwook; Carter, Bob S.; Hochberg, Fred H.; Breakefield, Xandra O.; Lee, Hakho; Weissleder, Ralph (Nature Pub. Group, 2015)
      Real-time monitoring of drug efficacy in glioblastoma multiforme (GBM) is a major clinical problem as serial re-biopsy of primary tumours is often not a clinical option. MGMT (O6-methylguanine DNA methyltransferase) and ...
    • Dopa-Responsive Dystonia: Functional Analysis of Single Nucleotide Substitutions within the 5’ Untranslated GCH1 Region 

      Armata, Ioanna A.; Balaj, Leonora; Kuster, John K.; Zhang, Xuan; Tsai, Shelun; Armatas, Andreas A.; Multhaupt-Buell, Trisha J.; Soberman, Roy; Breakefield, Xandra O.; Ichinose, Hiroshi; Sharma, Nutan (Public Library of Science, 2013)
      Background: Mutations in the GCH1 gene are associated with childhood onset, dopa-responsive dystonia (DRD). Correct diagnosis of DRD is crucial, given the potential for complete recovery once treated with L-dopa. The ...
    • Extracellular Vesicles and Their Convergence with Viral Pathways 

      Wurdinger, Thomas; Gatson, NaTosha N.; Balaj, Leonora; Kaur, Balveen; Breakefield, Xandra Owens; Pegtel, D. Michiel (Hindawi Publishing Corporation, 2012)
      Extracellular vesicles (microvesicles), such as exosomes and shed microvesicles, contain a variety of molecules including proteins, lipids, and nucleic acids. Microvesicles appear mostly to originate from multivesicular ...
    • Genetically Engineered Microvesicles Carrying Suicide mRNA/Protein Inhibit Schwannoma Tumor Growth 

      Mizrak, Arda; Bolukbasi, Mehmet Fatih; Ozdener, Gokhan Baris; Brenner, Gary Jay; Madlener, Sibylle; Erkan, Erdogan Pekcan; Ströbel, Thomas; Breakefield, Xandra Owens; Saydam, Okay (Nature Publishing Group, 2013)
      Microvesicles (MVs) play an important role in intercellular communication by carrying mRNAs, microRNAs (miRNAs), non-coding RNAs, proteins, and DNA from cell to cell. To our knowledge, this is the first report of delivery ...
    • Heparin affinity purification of extracellular vesicles 

      Balaj, Leonora; Atai, Nadia A.; Chen, Weilin; Mu, Dakai; Tannous, Bakhos A.; Breakefield, Xandra O.; Skog, Johan; Maguire, Casey A. (Nature Publishing Group, 2015)
      Extracellular vesicles (EVs) are lipid membrane vesicles released by cells. They carry active biomolecules including DNA, RNA, and protein which can be transferred to recipient cells. Isolation and purification of EVs from ...
    • A Highly Sensitive Assay for Monitoring the Secretory Pathway and ER Stress 

      Badr, Christian; Hewett, Jeffrey W.; Breakefield, Xandra Owens; Tannous, Bakhos A. (Public Library of Science, 2007)
      Background: The secretory pathway is a critical index of the capacity of cells to incorporate proteins into cellular membranes and secrete proteins into the extracellular space. Importantly it is disrupted in response to ...
    • In Vivo Tomographic Imaging of Red-Shifted Fluorescent Proteins 

      Deliolanis, Nikolaos C.; Wurdinger, Thomas; Pike, Lisa; Tannous, Bakhos A.; Breakefield, Xandra Owens; Weissleder, Ralph; Ntziachristos, Vasilis (Optical Society of America, 2011)
      We have developed a spectral inversion method for three-dimensional tomography of far-red and near-infrared fluorescent proteins in animals. The method was developed in particular to address the steep light absorption ...
    • Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium 

      Laurent, Louise C.; Abdel-Mageed, Asim B.; Adelson, P. David; Arango, Jorge; Balaj, Leonora; Breakefield, Xandra; Carlson, Elizabeth; Carter, Bob S.; Majem, Blanca; Chen, Clark C.; Cocucci, Emanuele; Danielson, Kirsty; Courtright, Amanda; Das, Saumya; Elmageed, Zakaria Y. Abd; Enderle, Daniel; Ezrin, Alan; Ferrer, Marc; Freedman, Jane; Galas, David; Gandhi, Roopali; Huentelman, Matthew J.; Van Keuren-Jensen, Kendall; Kalani, Yashar; Kim, Yong; Krichevsky, Anna M.; Lai, Charles; Lal-Nag, Madhu; Laurent, Clara D.; Leonardo, Trevor; Li, Feng; Malenica, Ivana; Mondal, Debasis; Nejad, Parham; Patel, Tushar; Raffai, Robert L.; Rubio, Renee; Skog, Johan; Spetzler, Robert; Sun, Jie; Tanriverdi, Kahraman; Vickers, Kasey; Wang, Liang; Wang, Yaoyu; Wei, Zhiyun; Weiner, Howard L.; Wong, David; Yan, Irene K.; Yeri, Ashish; Gould, Stephen (Co-Action Publishing, 2015)
      Extracellular RNAs (exRNAs) have been identified in all tested biofluids and have been associated with a variety of extracellular vesicles, ribonucleoprotein complexes and lipoprotein complexes. Much of the interest in ...
    • miR-1289 and “Zipcode”-like Sequence Enrich mRNAs in Microvesicles 

      Bolukbasi, Mehmet Fatih; Mizrak, Arda; Ozdener, Gokhan Baris; Madlener, Sibylle; Ströbel, Thomas; Erkan, Erdogan Pekcan; Fan, Jian-Bing; Breakefield, Xandra Owens; Saydam, Okay (Nature Publishing Group, 2012)
      Despite intensive studies, the molecular mechanisms by which the genetic materials are uploaded into microvesicles (MVs) are still unknown. This is the first study describing a zipcode-like 25 nucleotide (nt) sequence in ...
    • miR-21 in the Extracellular Vesicles (EVs) of Cerebrospinal Fluid (CSF): A Platform for Glioblastoma Biomarker Development 

      Akers, Johnny C.; Ramakrishnan, Valya; Kim, Ryan; Skog, Johan; Nakano, Ichiro; Pingle, Sandeep; Kalinina, Juliya; Hua, Wei; Kesari, Santosh; Mao, Ying; Breakefield, Xandra O.; Hochberg, Fred H.; Van Meir, Erwin G.; Carter, Bob S.; Chen, Clark C. (Public Library of Science, 2013)
      Glioblastoma cells secrete extra-cellular vesicles (EVs) containing microRNAs (miRNAs). Analysis of these EV miRNAs in the bio-fluids of afflicted patients represents a potential platform for biomarker development. However, ...
    • miRNA Signature of Schwannomas: Possible Role(s) of “Tumor Suppressor” miRNAs in Benign Tumors 

      Erkan, Erdogan Pekcan; Breakefield, Xandra Owens; Saydam, Okay (Impact Journals LLC, 2011)
      miRNAs have been recently implicated as drivers in several carcinogenic processes, where they can act either as oncogenes or as tumor suppressors. Schwannomas arise from Schwann cells, the myelinating cells of the peripheral ...
    • RNA Expression Patterns in Serum Microvesicles from Patients with Glioblastoma Multiforme and Controls 

      Noerholm, Mikkel; Balaj, Leonora; Limperg, Tobias; Salehi, Afshin; Zhu, Lin Dan; Hochberg, Fred Harvey; Breakefield, Xandra Owens; Carter, Bob S; Skog, Johan (BioMed Central, 2012)
      Background: RNA from exosomes and other microvesicles contain transcripts of tumour origin. In this study we sought to identify biomarkers of glioblastoma multiforme in microvesicle RNA from serum of affected patients. ...
    • Role of Exosomes/Microvesicles in the Nervous System and Use in Emerging Therapies 

      Lai, Charles Pin-Kuang; Breakefield, Xandra Owens (Frontiers Research Foundation, 2012)
      Extracellular membrane vesicles (EMVs) are nanometer sized vesicles, including exosomes and microvesicles capable of transferring DNAs, mRNAs, microRNAs, non-coding RNAs, proteins, and lipids among cells without direct ...
    • Stochastic Model of Tsc1 Lesions in Mouse Brain 

      Prabhakar, Shilpa; Goto, June; Zuang, Xuan; Sena-Esteves, Miguel; Bronson, Roderick Terry; Brockmann, Jillian; Gianni, Davide; Wojtkiewicz, Gregory R.; Chen, John Wen-Yueh; Stemmer-Rachamimov, Anat; Kwiatkowski, David Joseph; Breakefield, Xandra Owens (Public Library of Science, 2013)
      Tuberous sclerosis complex (TSC) is an autosomal dominant disorder due to mutations in either TSC1 or TSC2 that affects many organs with hamartomas and tumors. TSC-associated brain lesions include subependymal nodules, ...
    • TorsinA participates in endoplasmic reticulum-associated degradation 

      Nery, Flávia C.; Armata, Ioanna A.; Farley, Jonathan E.; Cho, JinAh; Yaqub, Uzma; Chen, Pan; da Hora, Cintia Carla; Wang, Qiuyan; Tagaya, Mitsuo; Klein, Christine; Tannous, Bakhos A.; Caldwell, Kim A.; Caldwell, Guy A.; Lencer, Wayne I.; Ye, Yihong; Breakefield, Xandra Owens (Nature Publishing Group, 2012)
      TorsinA is an \(AAA^+\) ATPase located within the lumen of the endoplasmic reticulum and nuclear envelope, with a mutant form causing early onset torsion dystonia (DYT1). Here we report a new function for torsinA in ...
    • Translating the Genomics Revolution: The Need for an International Gene Therapy Consortium for Monogenic Diseases 

      Tremblay, Jacques P; Xiao, Xiao; Aartsma-Rus, Annemieke; Barbas, Carlos; Blau, Helen M; Bogdanove, Adam J; Boycott, Kym; Braun, Serge; Breakefield, Xandra Owens; Bueren, Juan A; Buschmann, Michael; Byrne, Barry J; Calos, Michele; Cathomen, Toni; Chamberlain, Jeffrey; Chuah, Marinee; Cornetta, Kenneth; Davies, Kay E; Dickson, J George; Duchateau, Philippe; Flotte, Terence R; Gaudet, Daniel; Gersbach, Charles A; Gilbert, Renald; Glorioso, Joseph; Herzog, Roland W; High, Katherine A; Huang, Wenlin; Huard, Johnny; Joung, Jae Keith; Liu, Depei; Liu, Dexi; Lochmüller, Hanns; Lustig, Lawrence; Martens, Jeffrey; Massie, Bernard; Mavilio, Fulvio; Mendell, Jerry R; Nathwani, Amit; Ponder, Katherine; Porteus, Matthew; Puymirat, Jack; Samulski, Jude; Takeda, Shin'ichi; Thrasher, Adrian; VandenDriessche, Thierry; Wei, Yuquan; Wilson, James M; Wilton, Steve D; Wolfe, John H; Gao, Guangping (Nature Publishing Group, 2013)
    • Untethering the Nuclear Envelope and Cytoskeleton: Biologically Distinct Dystonias Arising from a Common Cellular Dysfunction 

      Atai, Nadia A.; Ryan, Scott D.; Kothary, Rashmi; Breakefield, Xandra Owens; Nery, Flávia C. (Hindawi Publishing Corporation, 2012)
      Most cases of early onset DYT1 dystonia in humans are caused by a GAG deletion in the TOR1A gene leading to loss of a glutamic acid (\(\Delta\)E) in the torsinA protein, which underlies a movement disorder associated with ...
    • Vesiclepedia: A Compendium for Extracellular Vesicles with Continuous Community Annotation 

      Kalra, Hina; Simpson, Richard J.; Ji, Hong; Aikawa, Elena; Altevogt, Peter; Askenase, Philip; Bond, Vincent C.; Borràs, Francesc E.; Breakefield, Xandra Owens; Budnik, Vivian; Buzas, Edit; Camussi, Giovanni; Clayton, Aled; Cocucci, Emanuele; Falcon-Perez, Juan M.; Gabrielsson, Susanne; Gho, Yong Song; Gupta, Dwijendra; Harsha, H. C.; Hendrix, An; Hill, Andrew F.; Inal, Jameel M.; Jenster, Guido; Krämer-Albers, Eva-Maria; Lim, Sai Kiang; Llorente, Alicia; Lötvall, Jan; Marcilla, Antonio; Mincheva-Nilsson, Lucia; Nazarenko, Irina; Nieuwland, Rienk; Nolte-'t Hoen, Esther N. M.; Pandey, Akhilesh; Patel, Tushar; Piper, Melissa G.; Pluchino, Stefano; Prasad, T. S. Keshava; Rajendran, Lawrence; Raposo, Graca; Record, Michel; Reid, Gavin E.; Sánchez-Madrid, Francisco; Schiffelers, Raymond M.; Siljander, Pia; Stensballe, Allan; Stoorvogel, Willem; Taylor, Douglas; Thery, Clotilde; Valadi, Hadi; van Balkom, Bas W. M.; Vázquez, Jesús; Vidal, Michel; Wauben, Marca H. M.; Yáñez-Mó, María; Zoeller, Margot; Mathivanan, Suresh (Public Library of Science, 2012)
      Extracellular vesicles (EVs) are membraneous vesicles released by a variety of cells into their microenvironment. Recent studies have elucidated the role of EVs in intercellular communication, pathogenesis, drug, vaccine ...
    • Visualization and tracking of tumour extracellular vesicle delivery and RNA translation using multiplexed reporters 

      Lai, Charles P.; Kim, Edward Y.; Badr, Christian E.; Weissleder, Ralph; Mempel, Thorsten R.; Tannous, Bakhos A.; Breakefield, Xandra O. (Nature Pub. Group, 2015)
      Accurate spatiotemporal assessment of extracellular vesicle (EV) delivery and cargo RNA translation requires specific and robust live-cell imaging technologies. Here we engineer optical reporters to label multiple EV ...