Browsing by Author "Olshansky, Lisa"
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Charge Transfer Dynamics at the α/β Subunit Interface of a Photochemical Ribonucleotide Reductase
Olshansky, Lisa; Stubbe, JoAnne; Nocera, Daniel (American Chemical Society (ACS), 2016)Ribonucleotide reductase (RNR) catalyzes the conversion of ribonucleotides to deoxyribonucleotides to provide the monomeric building blocks for DNA replication and repair. Nucleotide reduction occurs by way of multi-step ... -
Kinetics of Hydrogen Atom Abstraction from Substrate by an Active Site Thiyl Radical in Ribonucleotide Reductase
Olshansky, Lisa; Pizano, Arturo A.; Wei, Yifeng; Stubbe, JoAnne; Nocera, Daniel G. (American Chemical Society, 2014)Ribonucleotide reductases (RNRs) catalyze the conversion of nucleotides to deoxynucleotides in all organisms. Active E. coli class Ia RNR is an α2β2 complex that undergoes reversible, long-range proton-coupled electron ... -
Kinetics of Hydrogen Atom Abstraction from Substrate by an Active Site Thiyl Radical in Ribonucleotide Reductase
Olshansky, Lisa; Pizano, Arturo A.; Wei, Yifeng; Stubbe, JoAnne; Nocera, Daniel G. (American Chemical Society, 2014)Ribonucleotide reductases (RNRs) catalyze the conversion of nucleotides to deoxynucleotides in all organisms. Active E. coli class Ia RNR is an α2β2 complex that undergoes reversible, long-range proton-coupled electron ... -
Modulation of Y356 Photooxidation in E. coli Class Ia Ribonucleotide Reductase by Y731 Across the α2:β2 Interface
Pizano, Arturo A.; Olshansky, Lisa; Holder, Patrick G.; Stubbe, JoAnne; Nocera, Daniel (American Chemical Society (ACS), 2013)Substrate turnover in class Ia ribonucleotide reductase (RNR) requires reversible radical transport across two subunits over 35 A, which occurs by a multi-step proton-coupled electron transfer mechanism. Using a ... -
Photochemical Generation of a Tryptophan Radical within the Subunit Interface of Ribonucleotide Reductase
Olshansky, Lisa; Greene, Brandon Lee; Finkbeiner, Chelsea; Stubbe, JoAnne; Nocera, Daniel (American Chemical Society (ACS), 2016)The E. coli class Ia ribonucleotide reductase (RNR) achieves forward and reverse proton-coupled electron transfer (PCET) over a pathway of redox-active amino acids (β-Y122 ⇌ β-Y356 ⇌ α-Y731 ⇌ α-Y730 ⇌ α-C439) spanning ~35 ...