Now showing items 1-20 of 20

    • The Acid Test of Fluoride: How pH Modulates Toxicity 

      Sharma, Ramaswamy Narayanaswamy; Tsuchiya, Masahiro; Skobe, Ziedonis; Tannous, Bakhos A.; Bartlett, John D. (Public Library of Science, 2010)
      Background: It is not known why the ameloblasts responsible for dental enamel formation are uniquely sensitive to fluoride (\(F^−\)). Herein, we present a novel theory with supporting data to show that the low pH environment ...
    • Analysis of AKT and ERK1/2 protein kinases in extracellular vesicles isolated from blood of patients with cancer 

      van der Mijn, Johannes C.; Sol, Nik; Mellema, Wouter; Jimenez, Connie R.; Piersma, Sander R.; Dekker, Henk; Schutte, Lisette M.; Smit, Egbert F.; Broxterman, Henk J.; Skog, Johan; Tannous, Bakhos A.; Wurdinger, Thomas; Verheul, Henk M. W. (Co-Action Publishing, 2014)
      Background: Extracellular vesicles (EVs) are small nanometre-sized vesicles that are circulating in blood. They are released by multiple cells, including tumour cells. We hypothesized that circulating EVs contain protein ...
    • Attenuated AMPA Receptor Expression Allows Glioblastoma Cell Survival in Glutamate-Rich Environment 

      van Vuurden, Dannis G.; Yazdani, Maryam; Bosma, Ingeborg; Broekhuizen, Aart J. F.; Postma, Tjeerd J.; Heimans, Jan J.; van der Valk, Paul; Aronica, Eleonora; Würdinger, Thomas; Kaspers, Gertjan J. L.; Cloos, Jacqueline; Tannous, Bakhos A. (Public Library of Science, 2009)
      Background: Glioblastoma multiforme (GBM) cells secrete large amounts of glutamate that can trigger AMPA-type glutamate receptors (AMPARs). This commonly results in Na+ and Ca2+-permeability and thereby in excitotoxic cell ...
    • A chemical screen for medulloblastoma identifies quercetin as a putative radiosensitizer 

      Lagerweij, Tonny; Hiddingh, Lotte; Biesmans, Dennis; Crommentuijn, Matheus H.W.; Cloos, Jacqueline; Li, Xiao-Nan; Kogiso, Mari; Tannous, Bakhos A.; Vandertop, W. Peter; Noske, David P.; Kaspers, Gertjan J.L.; Würdinger, Tom; Hulleman, Esther (Impact Journals LLC, 2016)
      Treatment of medulloblastoma in children fails in approximately 30% of patients, and is often accompanied by severe late sequelae. Therefore, more effective drugs are needed that spare normal tissue and diminish long-term ...
    • EFEMP1 induces γ-secretase/Notch-mediated temozolomide resistance in glioblastoma 

      Hiddingh, Lotte; Tannous, Bakhos A.; Teng, Jian; Tops, Bas; Jeuken, Judith; Hulleman, Esther; Boots-Sprenger, Sandra H.; Vandertop, W. Peter; Noske, David P.; Kaspers, Gertjan J.L.; Wesseling, Pieter; Wurdinger, Thomas (Impact Journals LLC, 2014)
      Glioblastoma is the most common malignant primary brain tumor. Temozolomide (TMZ) is the standard chemotherapeutic agent for this disease. However, intrinsic and acquired TMZ-resistance represents a major obstacle for this ...
    • Functional Multiplex Reporter Assay Using Tagged Gaussia Luciferase 

      van Rijn, Sjoerd; Nilsson, Jonas; Noske, David P.; Vandertop, W. Peter; Tannous, Bakhos A.; Würdinger, Thomas (Nature Publishing Group, 2013)
      We have developed a multiplex reporter system to monitor multiple biological variables in real-time. The secreted Gaussia luciferase was fused to ten different epitope tags (Gluc\(_{tag}\)), each expressed in different ...
    • Heparin affinity purification of extracellular vesicles 

      Balaj, Leonora; Atai, Nadia A.; Chen, Weilin; Mu, Dakai; Tannous, Bakhos A.; Breakefield, Xandra O.; Skog, Johan; Maguire, Casey A. (Nature Publishing Group, 2015)
      Extracellular vesicles (EVs) are lipid membrane vesicles released by cells. They carry active biomolecules including DNA, RNA, and protein which can be transferred to recipient cells. Isolation and purification of EVs from ...
    • A Highly Sensitive Assay for Monitoring the Secretory Pathway and ER Stress 

      Badr, Christian; Hewett, Jeffrey W.; Breakefield, Xandra Owens; Tannous, Bakhos A. (Public Library of Science, 2007)
      Background: The secretory pathway is a critical index of the capacity of cells to incorporate proteins into cellular membranes and secrete proteins into the extracellular space. Importantly it is disrupted in response to ...
    • Imaging Tumor Vascularity and Response to Anti-Angiogenic Therapy Using Gaussia Luciferase 

      Kantar, Rami S.; Lashgari, Ghazal; Tabet, Elie I.; Lewandrowski, Grant K.; Carvalho, Litia A.; Tannous, Bakhos A. (Nature Publishing Group, 2016)
      We developed a novel approach to assess tumor vascularity using recombinant Gaussia luciferase (rGluc) protein and bioluminescence imaging. Upon intravenous injection of rGluc followed by its substrate coelenterazine, ...
    • In Vivo Tomographic Imaging of Red-Shifted Fluorescent Proteins 

      Deliolanis, Nikolaos C.; Wurdinger, Thomas; Pike, Lisa; Tannous, Bakhos A.; Breakefield, Xandra Owens; Weissleder, Ralph; Ntziachristos, Vasilis (Optical Society of America, 2011)
      We have developed a spectral inversion method for three-dimensional tomography of far-red and near-infrared fluorescent proteins in animals. The method was developed in particular to address the steep light absorption ...
    • Multimodal targeted high relaxivity thermosensitive liposome for in vivo imaging 

      Kuijten, Maayke M. P.; Hannah Degeling, M.; Chen, John W.; Wojtkiewicz, Gregory; Waterman, Peter; Weissleder, Ralph; Azzi, Jamil; Nicolay, Klaas; Tannous, Bakhos A. (Nature Publishing Group, 2015)
      Liposomes are spherical, self-closed structures formed by lipid bilayers that can encapsulate drugs and/or imaging agents in their hydrophilic core or within their membrane moiety, making them suitable delivery vehicles. ...
    • A neural basis for melanocortin-4 receptor regulated appetite 

      Garfield, Alastair S.; Li, Chia; Madara, Joseph C.; Shah, Bhavik P.; Webber, Emily; Steger, Jennifer S.; Campbell, John N.; Gavrilova, Oksana; Lee, Charlotte E.; Olson, David P.; Elmquist, Joel K.; Tannous, Bakhos A.; Krashes, Michael J.; Lowell, Bradford B. (2015)
      Pro-opiomelanocortin (POMC)- and agouti-related peptide (AgRP)-expressing neurons are oppositely regulated by caloric depletion and co-ordinately stimulate and inhibit homeostatic satiety, respectively. This bimodality is ...
    • Pharmacokinetics of Natural and Engineered Secreted Factors Delivered by Mesenchymal Stromal Cells 

      Elman, Jessica S.; Murray, Ryan M.; Wang, Fangjing; Shen, Keyue; Gao, Shan; Conway, Kevin E.; Yarmush, Martin L.; Tannous, Bakhos A.; Weissleder, Ralph; Parekkadan, Biju (Public Library of Science, 2014)
      Transient cell therapy is an emerging drug class that requires new approaches for pharmacological monitoring during use. Human mesenchymal stem cells (MSCs) are a clinically-tested transient cell therapeutic that naturally ...
    • Rearranged EML4-ALK fusion transcripts sequester in circulating blood platelets and enable blood-based crizotinib response monitoring in non-small-cell lung cancer 

      Nilsson, R. Jonas A.; Karachaliou, Niki; Berenguer, Jordi; Gimenez-Capitan, Ana; Schellen, Pepijn; Teixido, Cristina; Tannous, Jihane; Kuiper, Justine L.; Drees, Esther; Grabowska, Magda; van Keulen, Marte; Heideman, Danielle A.M.; Thunnissen, Erik; Dingemans, Anne-Marie C.; Viteri, Santiago; Tannous, Bakhos A.; Drozdowskyj, Ana; Rosell, Rafael; Smit, Egbert F.; Wurdinger, Thomas (Impact Journals LLC, 2016)
      Purpose: Non-small-cell lung cancers harboring EML4-ALK rearrangements are sensitive to crizotinib. However, despite initial response, most patients will eventually relapse, and monitoring EML4-ALK rearrangements over the ...
    • Secreted Gaussia Luciferase as a Biomarker for Monitoring Tumor Progression and Treatment Response of Systemic Metastases 

      Chung, Euiheon; Yamashita, Hiroshi; Au, Patrick; Tannous, Bakhos A.; Fukumura, Dai; Jain, Rakesh K. (Public Library of Science, 2009)
      Background: Currently, only few techniques are available for quantifying systemic metastases in preclinical model. Thus techniques that can sensitively detect metastatic colonization and assess treatment response in real-time ...
    • Systemically administered AAV9-sTRAIL combats invasive glioblastoma in a patient-derived orthotopic xenograft model 

      Crommentuijn, Matheus HW; Kantar, Rami; Noske, David P; Vandertop, W Peter; Badr, Christian E; Würdinger, Thomas; Maguire, Casey A; Tannous, Bakhos A (Nature Publishing Group, 2016)
      Adeno-associated virus (AAV) vectors expressing tumoricidal genes injected directly into brain tumors have shown some promise, however, invasive tumor cells are relatively unaffected. Systemic injection of AAV9 vectors ...
    • Therapeutic potential of targeting microRNA‐10b in established intracranial glioblastoma: first steps toward the clinic 

      Teplyuk, Nadiya M; Uhlmann, Erik J; Gabriely, Galina; Volfovsky, Natalia; Wang, Yang; Teng, Jian; Karmali, Priya; Marcusson, Eric; Peter, Merlene; Mohan, Athul; Kraytsberg, Yevgenya; Cialic, Ron; Chiocca, E Antonio; Godlewski, Jakub; Tannous, Bakhos; Krichevsky, Anna M (John Wiley and Sons Inc., 2016)
      Abstract MicroRNA‐10b (miR‐10b) is a unique oncogenic miRNA that is highly expressed in all GBM subtypes, while absent in normal neuroglial cells of the brain. miR‐10b inhibition strongly impairs proliferation and survival ...
    • TorsinA participates in endoplasmic reticulum-associated degradation 

      Nery, Flávia C.; Armata, Ioanna A.; Farley, Jonathan E.; Cho, JinAh; Yaqub, Uzma; Chen, Pan; da Hora, Cintia Carla; Wang, Qiuyan; Tagaya, Mitsuo; Klein, Christine; Tannous, Bakhos A.; Caldwell, Kim A.; Caldwell, Guy A.; Lencer, Wayne I.; Ye, Yihong; Breakefield, Xandra Owens (Nature Publishing Group, 2012)
      TorsinA is an \(AAA^+\) ATPase located within the lumen of the endoplasmic reticulum and nuclear envelope, with a mutant form causing early onset torsion dystonia (DYT1). Here we report a new function for torsinA in ...
    • Triple Bioluminescence Imaging for In Vivo Monitoring of Cellular Processes 

      Maguire, Casey A; Bovenberg, M Sarah; Crommentuijn, Matheus HW; Niers, Johanna M; Kerami, Mariam; Teng, Jian; Sena-Esteves, Miguel; Badr, Christian E; Tannous, Bakhos A (Nature Publishing Group, 2013)
      Bioluminescence imaging (BLI) has shown to be crucial for monitoring in vivo biological processes. So far, only dual bioluminescence imaging using firefly (Fluc) and Renilla or Gaussia (Gluc) luciferase has been achieved ...
    • Visualization and tracking of tumour extracellular vesicle delivery and RNA translation using multiplexed reporters 

      Lai, Charles P.; Kim, Edward Y.; Badr, Christian E.; Weissleder, Ralph; Mempel, Thorsten R.; Tannous, Bakhos A.; Breakefield, Xandra O. (Nature Pub. Group, 2015)
      Accurate spatiotemporal assessment of extracellular vesicle (EV) delivery and cargo RNA translation requires specific and robust live-cell imaging technologies. Here we engineer optical reporters to label multiple EV ...