Browsing by Author "Jia, Tony Z."
Now showing items 1-3 of 3
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Digital RNA Sequencing Minimizes Sequence-Dependent Bias and Amplification Noise with Optimized Single-Molecule Barcodes
Shiroguchi, Katsuyuki; Jia, Tony Z; Sims, Peter A.; Xie, Xiaoliang Sunney (Proceedings of the National Academy of Sciences, 2012)RNA sequencing (RNA-Seq) is a powerful tool for transcriptome profiling, but is hampered by sequence-dependent bias and inaccuracy at low copy numbers intrinsic to exponential PCR amplification. We developed a simple ... -
Peptide-Assisted Nonenzymatic RNA Replication in Coacervate Droplets
Jia, Tony Z. (2016-09-14)Life on earth may have first appeared in the form of a protocell capable of metabolism and containing a self-replicating informational polymer. This polymer was likely to have been RNA due to its ability to serve both as ... -
Rapid RNA Exchange in Aqueous Two-Phase System and Coacervate Droplets
Jia, Tony Z.; Hentrich, Christian; Szostak, Jack W. (Springer Netherlands, 2014)Compartmentalization in a prebiotic setting is an important aspect of early cell formation and is crucial for the development of an artificial protocell system that effectively couples genotype and phenotype. Aqueous ...