Now showing items 21-28 of 28

    • Recipient Cell Nuclear Factors Are Required for Reprogramming by Nuclear Transfer 

      Egli, Dieter; Eggan, Kevin Carl (Company of Biologists, 2010)
      Nuclear transfer allows the reprogramming of somatic cells to totipotency. The cell cycle state of the donor and recipient cells, as well as their extent of differentiation, have each been cited as important determinants ...
    • Reprogramming after Chromosome Transfer into Mouse Blastomeres 

      Egli, Dieter; Sandler, Vladislav M.; Shinohara, Mari L.; Cantor, Harvey Ira; Eggan, Kevin Carl (Elsevier BV, 2009)
      It is well known that oocytes can reprogram differentiated cells, allowing animal cloning by nuclear transfer. We have recently shown that fertilized zygotes retain reprogramming activities [1], suggesting that such ...
    • Reprogramming within Hours Following Nuclear Transfer into Mouse but not Human Zygotes 

      Egli, Dieter; Chen, Alice E.; Saphier Belfer, Genevieve C; Ichida, Justin; Fitzgerald, Claire; Go, Kathryn J.; Acevedo, Nicole; Patel, Jay; Baetscher, Manfred; Kearns, William G.; Goland, Robin; Leibel, Rudolph L.; Melton, Douglas A.; Eggan, Kevin Carl (Nature Publishing Group, 2011)
      Fertilized mouse zygotes can reprogram somatic cells to a pluripotent state. Human zygotes might therefore be useful for producing patient-derived pluripotent stem cells. However, logistical, legal and social considerations ...
    • The role of maternal-specific H3K9me3 modification in establishing imprinted X-chromosome inactivation and embryogenesis in mice 

      Fukuda, Atsushi; Tomikawa, Junko; Miura, Takumi; Hata, Kenichiro; Nakabayashi, Kazuhiko; Eggan, Kevin; Akutsu, Hidenori; Umezawa, Akihiro (Nature Pub. Group, 2014)
      Maintaining a single active X-chromosome by repressing Xist is crucial for embryonic development in mice. Although the Xist activator RNF12/RLIM is present as a maternal factor, maternal Xist (Xm-Xist) is repressed during ...
    • A small molecule inhibitor of Tgf-β signaling replaces Sox2 in reprogramming by inducing Nanog 

      Ichida, Justin K.; Blanchard, Joel; Lam, Kelvin; Son, Esther Y.; Chung, Julia E.; Egli, Dieter; Loh, Kyle M.; Carter, Ava C.; Di Giorgio, Francesco P.; Koszka, Kathryn E; Huangfu, Danwei; Akutsu, Hidenori; Liu, David Ruchien; Rubin, Lee; Eggan, Kevin Carl (Elsevier BV, 2009)
      The combined activity of three transcription factors can reprogram adult cells into induced pluripotent stem (iPS) cells. However, the transgenic methods used to deliver reprogramming factors have raised concerns regarding ...
    • Somatic coding mutations in human induced pluripotent stem cells 

      Gore, A; Li, Z; Fung, HL; Young, J; Agarwal, Suneet; Antosiewicz-Bourget, J; Canto, I; Israel, M; Kiskinis, E; Lee, JH; Loh, YH; Manos, P; Montserrat, N; Wilbert, M; Yu, J; Kirkness, E; Belmonte, JCI; Daley, GQ; Eggan, Kevin Carl; Rossi, DJ; Thomson, J; Goldstein, LSB; Zhang, K (Springer Nature, 2011)
      Defined transcription factors can induce epigenetic reprogramming of adult mammalian cells into induced pluripotent stem cells. Although DNA factors are integrated during some reprogramming methods, it is unknown whether ...
    • Sox17 promotes differentiation in mouse embryonic stem cells by directly regulating extraembryonic gene expression and indirectly antagonizing self-renewal 

      Niakan, K. K.; Ji, H.; Maehr, R.; Vokes, S. A.; Rodolfa, K. T.; Sherwood, Richard Irving; Yamaki, M.; Dimos, J. T.; Chen, A. E.; Melton, Douglas A.; McMahon, Andrew P.; Eggan, Kevin Carl (Cold Spring Harbor Laboratory Press, 2010)
      In embryonic stem (ES) cells, a well-characterized transcriptional network promotes pluripotency and represses gene expression required for differentiation. In comparison, the transcriptional networks that promote ...
    • Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming 

      Deng, Jie; Shoemaker, Robert; Xie, Bin; Gore, Athurva; LeProust, Emily M; Antosiewicz-Bourget, Jessica; Egli, Dieter; Maherali, Nimet A; Park, In-Hyun; Yu, Junying; Daley, George Quentin; Eggan, Kevin Carl; Hochedlinger, Konrad; Thomson, James; Wang, Wei Li; Gao, Yuan; Zhang, Kun (Nature Publishing Group, 2009)
      Current DNA methylation assays are limited in the flexibility and efficiency of characterizing a large number of genomic targets. We report a method to specifically capture an arbitrary subset of genomic targets for ...