Now showing items 21-30 of 30

    • PPIRank - an advanced method for ranking protein-protein interations in TAP/MS data 

      Sun, Xiaoyun; Hong, Pengyu; Kulkarni, Meghana; Kwon, Young; Perrimon, Norbert (BioMed Central, 2013)
      Background: Tandem affinity purification coupled with mass-spectrometry (TAP/MS) analysis is a popular method for the identification of novel endogenous protein-protein interactions (PPIs) in large-scale. Computational ...
    • Regulators of Autophagosome Formation in Drosophila Muscles 

      Zirin, Jonathan; Nieuwenhuis, Joppe; Samsonova, Anastasia; Tao, Rong; Perrimon, Norbert (Public Library of Science, 2015)
      Given the diversity of autophagy targets and regulation, it is important to characterize autophagy in various cell types and conditions. We used a primary myocyte cell culture system to assay the role of putative autophagy ...
    • Role of Autophagy in Glycogen Breakdown and Its Relevance to Chloroquine Myopathy 

      Zirin, Jonathan; Nieuwenhuis, Joppe; Perrimon, Norbert (Public Library of Science, 2013)
      Several myopathies are associated with defects in autophagic and lysosomal degradation of glycogen, but it remains unclear how glycogen is targeted to the lysosome and what significance this process has for muscle cells. ...
    • Roles of Major Facilitator Superfamily Transporters in Phosphate Response in \(Drosophila\) 

      Bergwitz, Clemens; Rasmussen, Matthew D.; DeRobertis, Charles; Wee, Mark J.; Sinha, Sumi; Chen, Hway H.; Huang, Joanne; Perrimon, Norbert (Public Library of Science, 2012)
      The major facilitator superfamily (MFS) transporter \(Pho84\) and the type III transporter \(Pho84\) are responsible for metabolic effects of inorganic phosphate in yeast. While the \(Pho84\) ortholog Pit1 was also shown ...
    • Sarcomere Formation Occurs by the Assembly of Multiple Latent Protein Complexes 

      Rui, Yanning; Bai, Jianwu; Perrimon, Norbert (Public Library of Science, 2010)
      The stereotyped striation of myofibrils is a conserved feature of muscle organization that is critical to its function. Although most components that constitute the basic myofibrils are well-characterized biochemically and ...
    • A Screen for Morphological Complexity Identifies Regulators of Switch-like Transitions between Discrete Cell Shapes 

      Yin, Zheng; Sadok, Amine; Sailem, Heba; McCarthy, Afshan; Xia, Xiaofeng; Li, Fuhai; Garcia, Mar Arias; Evans, Louise; Barr, Alexis; Perrimon, Norbert; Marshall, Christopher J; Wong, Stephen T.C.; Bakal, Chris (2013)
      The way in which cells adopt different morphologies is not fully understood. Cell shape could be a continuous variable or restricted to a set of discrete forms. We developed quantitative methods to describe cell shape and ...
    • A sharp end to sugary Wingless travels 

      Droujinine, Ilia A.; Yan, Dong; Perrimon, Norbert (The Rockefeller University Press, 2014)
      Drosophila melanogaster follicle stem cells are controlled by Wingless (Wg) ligands secreted 50 µm away, raising the question of how long-distance Wg spreading occurs. In this issue of JCB, Wang and Page-McCaw (2014. J. ...
    • A Systems-Level Interrogation Identifies Regulators of Drosophila Blood Cell Number and Survival 

      Sopko, Richelle; Lin, You Bin; Makhijani, Kalpana; Alexander, Brandy; Perrimon, Norbert; Brückner, Katja (Public Library of Science, 2015)
      In multicellular organisms, cell number is typically determined by a balance of intracellular signals that positively and negatively regulate cell survival and proliferation. Dissecting these signaling networks facilitates ...
    • A transgenic resource for conditional competitive inhibition of conserved Drosophila microRNAs 

      Fulga, Tudor A.; McNeill, Elizabeth M.; Binari, Richard; Yelick, Julia; Blanche, Alexandra; Booker, Matthew; Steinkraus, Bruno R.; Schnall-Levin, Michael; Zhao, Yong; DeLuca, Todd; Bejarano, Fernando; Han, Zhe; Lai, Eric C.; Wall, Dennis P.; Perrimon, Norbert; Van Vactor, David (Nature Pub. Group, 2015)
      Although the impact of microRNAs (miRNAs) in development and disease is well established, understanding the function of individual miRNAs remains challenging. Development of competitive inhibitor molecules such as miRNA ...
    • Using Iterative Cluster Merging with Improved Gap Statistics to Perform Online Phenotype Discovery in the Context of High-throughput RNAi Screens 

      Yin, Zheng; Zhou, Xiaobo; Bakal, Chris; Li, Fuhai; Sun, Youxian; Perrimon, Norbert; Wong, Stephen TC (BioMed Central, 2008)
      Background: The recent emergence of high-throughput automated image acquisition technologies has forever changed how cell biologists collect and analyze data. Historically, the interpretation of cellular phenotypes in ...